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Chicken mycoplasma synoviae inactivated vaccine

A technology of mycoplasma synovialum and inactivated vaccines, which is applied in vaccines, veterinary vaccines, antibody medical components, etc., can solve the problems of limited application and achieve the effects of reduced incidence, high duration, and increased abundance

Active Publication Date: 2022-01-21
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the attenuated MS vaccine has some disadvantages
It can only be applied to chicken farms without MS infection, which largely limits its application
In addition, the attenuated MS vaccine has the risk of horizontal transmission and may be transmitted among pens on the same farm, resulting in MS infection in unvaccinated birds

Method used

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  • Chicken mycoplasma synoviae inactivated vaccine
  • Chicken mycoplasma synoviae inactivated vaccine
  • Chicken mycoplasma synoviae inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The isolation and identification of embodiment 1 mycoplasma synovial bursa

[0027] 1 Isolation of mycoplasma in chicken synovial bursa

[0028] Collect the contents of the swollen tarsal joint cavity of chickens suspected of being infected with Mycoplasma gallisanum from Fuzhou chicken farms, inoculate them in modified Frey's liquid medium under aseptic conditions, and place them in an incubator at 37°C for 48 hours with constant temperature shaking. When the bacterial liquid changed from red to yellow, it was filtered with a 0.45 μm filter, and the filtrate was inoculated into the modified Frey's liquid medium at a ratio of 1:10 for cultivation, and the second-generation bacterial liquid was used for identification of pathogenic bacteria.

[0029] 2 Identification of Mycoplasma bursae

[0030] 2.1 Morphological identification of bacteria

[0031] The smear of the second-generation bacterial liquid was taken and observed under the microscope after Giemsa staining. Th...

Embodiment 2

[0056] Embodiment 2, the preparation of chicken synovial bursa mycoplasma inactivated vaccine

[0057] 2.1 Preparation of seed solution for production

[0058] According to the ratio of 1:10, add Mycoplasma synovialum MS-FJ01 to the medium of Mycoplasma chicken synovialum, and culture at 37°C at a constant temperature of 180r / min to the logarithmic growth phase (the bacterial solution changes from red to yellow) ), collect the bacterial liquid as seed liquid, and store at 4°C.

[0059] Among them, the preparation method of the mycoplasma bursae culture medium is: 5.6g of yeast extract powder, 21g of brain heart extract broth, 8mL of 50mg / mL arginine solution, 2mL of 50mg / mL L-cysteine ​​solution , 2 mL of 1 wt % phenol red solution and 0.4 mL of 250 mg / mL coenzyme I solution, dissolved in deionized water and fixed to 900 mL, then added 800,000 units / L of penicillin and 100 mL of inactivated fetal bovine serum, Adjust the pH to 7.6-7.8, filter through a 0.22 μm filter into a ...

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Abstract

The invention discloses a chicken mycoplasma synoviae inactivated vaccine which comprises an antigen and a vaccine adjuvant, and the antigen is an inactivated mycoplasma synoviae MS-FJ01 strain; the strain is separated from sick chicken attached joints with chicken mycoplasma synoviae in Fuzhou, and belongs to a typical strain in Fujian province; the strain is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan, China, the preservation number is CCTCC NO: M 2021210, and the preservation date is March 8, 2021; according to the scheme, the vaccine can generate a high-level antibody, the immunization duration is long, the morbidity of the immunized chicken mycoplasma synoviae is obviously reduced, and the prevalence of the chicken mycoplasma synoviae can be effectively prevented; the chicken mycoplasma synoviae inactivated vaccine prepared from the mycoplasma synoviae MS-FJ01 strain in the scheme can effectively prevent and control the chicken mycoplasma synoviae, the richness of commercially available vaccines of the category is improved, and an optional scheme is provided.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to an inactivated vaccine of Mycoplasma gallinarum. Background technique [0002] Mycoplasma synoviae (MS) mainly causes poultry upper respiratory tract disease, air sacculitis, bursitis, lameness, paralysis and so on. Although the lethality rate is very low, once it is infected in the flock, it is difficult to eradicate it, resulting in stunted growth and decreased egg production in the flock for a long time. Moreover, MS can spread vertically, which brings great difficulties to the eradication of MS. At present, MS exists widely in chicken farms all over the world, and MS is prone to cross-infection with other pathogens, aggravating the pathogenicity of other pathogens, and causing huge economic losses to the chicken industry. [0003] At present, the main measures to prevent and control the harm of MS are the use of chicken farm decontamination, antibioti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/35
CPCC12N1/20A61K39/0241A61P31/04A61K2039/521A61K2039/552Y02A50/30
Inventor 陈吉龙李训良
Owner FUJIAN AGRI & FORESTRY UNIV
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