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Dendrobium fermentation product and preparation method and application thereof

A technology of fermented products and dendrobium, applied in the direction of bacteria used in food preparation, application, food science, etc., can solve the problems of complex ingredients, single fermentation method, high content of auxiliary materials, etc.

Pending Publication Date: 2022-01-25
ANGEL NUTRITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The technical problems existing in the prior art are: in the prior art, the strains used for fermentation and the fermentation process are not comprehensive, the strains used for fermentation, the fermentation method are relatively single, and a large amount of auxiliary materials are used as fermentation substrates in many fermentation processes , the content of such excipients is high and the composition is complex. Although the final fermentation liquid has efficacy, it cannot represent the true characteristics of Dendrobium, nor is it the performance of Dendrobium after fermentation.

Method used

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  • Dendrobium fermentation product and preparation method and application thereof
  • Dendrobium fermentation product and preparation method and application thereof
  • Dendrobium fermentation product and preparation method and application thereof

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preparation example Construction

[0091] In another aspect, the invention provides a method for preparing a dendrobium fermented product, comprising the steps of:

[0092] 1) cell activation culture;

[0093] 2) Dendrobium raw material processing;

[0094] 3) enzymatic hydrolysis of the dendrobium treated in step (2);

[0095] 4) inoculating and fermenting the material after enzymolysis in step (3);

[0096] 5) Subsequent processing.

[0097] In a preferred embodiment of the present invention, the fermentation method in step (4) includes: separate fermentation of various microorganisms alone, mixed simultaneous fermentation, mixed use after separate fermentation, and step-by-step gradient fermentation.

[0098] In a preferred embodiment of the present invention, step (5) includes centrifugation and filtration to obtain a clear liquid, and / or freeze-drying or spray-drying to obtain a powder product.

[0099] In addition, the present invention also provides a fermented product of dendrobium for external use ...

Embodiment 1

[0121] (1) Activation culture of strains:

[0122] Saccharomyces boularidi Angel 1.27 (Saccharomyces boularidi Angel 1.27), the preservation number is CCTCCNO: M2012116, a ring of bacterial colonies in the slant is picked and put into 500mL PBD liquid medium, and the shaker culture is carried out, wherein the temperature of the shaker culture is 28°C, activate culture on a shaker for 16 hours, and centrifuge at a centrifugal rate of 5000r / min for 10min to obtain yeast cells;

[0123] Among them, the composition of PDB liquid medium includes: water 1L, potato extract 4.0g, glucose 20.0g, and adjust the pH to 5.6±0.2;

[0124] After testing, the OD value was 0.8 when the culture was completed.

[0125] The test method of OD value specifically includes: spectrophotometer, visible light region A=600nm, using pure water as a reference reagent to directly measure OD value.

[0126] (2) Dendrobium raw material processing:

[0127] The fresh dendrobium candidum stems are washed and...

Embodiment 2

[0137] (1) Activation culture of strains:

[0138] A. Saccharomyces cerevisiae activation: take Saccharomyces cerevisiae: Saccharomyces cerevisiae A3.12 (Saccharomyces cerevisiaeA3.12), pick a ring of colonies in the slope and put it into PDB liquid medium, activate and cultivate at 30°C on a shaking table, and the shaking table speed is 200r / min , the shaker culture was completed at 20 h, and the yeast cells were obtained by centrifugation.

[0139] After testing, the OD value was 0.7 when the culture was completed.

[0140] Among them, the composition of PDB liquid medium includes: water 1L, potato extract 4.0g, glucose 20.0g, and adjust the pH to 5.6±0.2;

[0141] B. Activation of acetic acid bacteria: Acetic acid bacteria: Komagataeibactersaccharivorans AYC1.2 (Komagataeibactersaccharivorans AYC1,2), the preservation number is CCTCC NO: M2019569, pick a ring into the liquid medium, and activate the shaker at 30°C for 24h. centrifuged to obtain acetic acid bacteria cells,...

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Abstract

The invention relates to the field of additives, and particularly relates to a dendrobium fermentation product and a preparation method and application thereof. On one hand, the dendrobium fermentation product with oxidability and immunity improvement is provided, and the dendrobium fermentation product is prepared from dendrobium, an enzymolysis agent and fermentation bacteria. On the other hand, the invention further provides the preparation method of the dendrobium fermentation product. The preparation method comprises the steps of fermentation bacteria activation, dendrobium raw material treatment, enzymolysis, fermentation and aftertreatment. The dendrobium fermentation product capable of being taken orally and used externally is prepared, and release of polyphenol, polysaccharide and flavonoid substances in dendrobium and enrichment of microbial metabolites such as short-chain fatty acid, polypeptide and amino acid are achieved. The fermented dendrobium has the special fermentation aroma and good taste, and the synergistic interaction effect of oral administration and external use can be achieved.

Description

technical field [0001] The invention relates to the field of additives, in particular to a dendrobium fermented product and a preparation method and application thereof. Background technique [0002] Dendrobium (Dendrobium), the second largest genus of Orchidaceous plants, the traditional Chinese medicine Dendrobium is a perennial herb, there are about 500 genera and more than 1,500 species in the world, and there are about 76 species in China, of which 33 species are used as the original source of commercial Dendrobium. Plants are acquired for circulation. In 2005, "Pharmacopoeia of the People's Republic of China" included three kinds of Dendrobium that can be used as traditional Chinese medicine, including: Tiepi Shijie, Nobile Dendrobium, and Vertebrae Dendrobium. The three kinds of Dendrobium included in the Pharmacopoeia of the People's Republic of China in 2010 and 2015 are the stems of Dendrobium nobile, Dendrobium drumstick and Dendrobium fringe, while Dendrobium ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A23L29/00A61K8/9794A61Q19/08A61Q19/00A61K36/8984A61P39/06A61P1/00A61P37/04
CPCA23L33/00A23L29/06A23L29/065A61K8/9794A61Q19/08A61Q19/00A61K36/8984A61P39/06A61P1/00A61P37/04A23V2002/00A61K2800/782A61K2236/19A23V2400/125A23V2400/113A23V2400/167A23V2400/175A23V2400/519A23V2400/533A23V2400/169A23V2200/32A23V2200/324A23V2250/76
Inventor 彭宁熊涛李知洪覃敬羽张彦张海波王欢杨树金冯玖
Owner ANGEL NUTRITECH CO LTD
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