Application of boswellia serrata resin in preparation of product for promoting proliferation of intestinal beneficial bacteria
A technology of Boswellia serrata and beneficial bacteria is applied in the field of microorganisms to achieve the effect of promoting the proliferation and efficient preparation of intestinal beneficial bacteria
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[0039] Carry out in vivo simulation experiments in vitro, take human feces as intestinal flora samples, and observe the effect of Boswellia serrata resin intervention on intestinal flora, the specific operation process is as follows:
[0040]1. Fecal sample processing
[0041] (1) PBS buffer preparation
[0042] Taking 1000ml as an example, weigh the following medicines with an electronic analytical balance and pour them into a 1000ml beaker, including: KH 2 PO 4 0.24g, Na 2 HPO 4 12H 2 O 2.90g, NaCl 8.00g, KCl 0.20g.
[0043] Use a graduated cylinder to measure 800ml of ultra-pure water and pour it into a beaker, put it into the stirring rotor, and put it on a magnetic heating stirrer to stir until it is completely dissolved, then use a calibrated pH meter to measure the pH of the above solution, use 0.1M HCl or NaOH adjusted pH=7.4±0.05.
[0044] Drain the above-mentioned completely dissolved and adjusted pH solution into a 1000ml volumetric flask with a glass rod, r...
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