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Construction method for liver portal vein ischemia injury model

A construction method and technology of ischemic injury, applied in the fields of pharmaceutical formulations, preparations for in vivo experiments, medical science, etc., can solve problems that have not yet been seen, such as the preparation of liver portal vein ischemic injury models, and achieve reduced drug consumption and good application The effect of foreground, construction method stabilization

Pending Publication Date: 2022-02-08
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no use of portal vein ligation to create a model of hepatic portal vein ischemia injury.

Method used

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  • Construction method for liver portal vein ischemia injury model
  • Construction method for liver portal vein ischemia injury model
  • Construction method for liver portal vein ischemia injury model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the construction of the liver portal vein ischemic injury model of the present invention

[0043] The construction method of the liver portal vein ischemia injury model of the present invention comprises the following steps:

[0044] (1) Select C57BL / 6 mice (either male or female), aged 4 to 6 weeks, weighing 22 to 26 g, and raised them in a constant temperature and humidity animal room with a 12-hour day and night cycle, and provided standard feed and water ad libitum.

[0045] (2) Fasting for 4 hours before operation, anesthesia with special small animal gas anesthesia machine. After rapid induction of anesthesia with isoflurane, 3% isoflurane mixed with air was given for continuous inhalation (flow rate 0.6L / min) until the end of the operation.

[0046] (3) The mice were fixed in the supine position, the hair on the abdomen of the mice was shaved off, and the abdomen was opened with an incision in the middle, and the length of the incision was about 2....

Embodiment 2

[0063] Embodiment 2, using the liver portal vein ischemic injury model of the present invention to screen drugs

[0064] (1) Prepare the liver portal vein ischemia injury model according to Example 1;

[0065] (2) applying the candidate drug to the liver portal vein ischemic injury model established in step (1);

[0066] (3) To observe the effects of candidate drugs on various indicators of liver damage, and to evaluate potential therapeutic drugs for liver damage.

Embodiment 3

[0067] Example 3. Other applications of the liver portal vein ischemic injury model of the present invention

[0068] The liver portal vein ischemic injury model prepared according to Example 1 can be used to study the pathogenesis of liver injury after TIPS.

[0069] In summary, the present invention provides a method for constructing a liver portal vein ischemia injury model. The construction method adopts conventional techniques in the field, does not require the use of precision instruments and consumables, and does not require complicated surgical operations. The operation is simple and easy to construct. , the modeling time is short, and the liver portal vein ischemic injury model can be constructed within 6 hours, and it is suitable for small animals, with strong applicability and low cost; at the same time, the construction method is very stable, and the construction of the liver portal vein ischemic injury model is highly reproducible . The invention is convenient fo...

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Abstract

The invention provides a construction method of a liver portal vein ischemia injury model, belonging to the field of animal model construction. According to the construction method, a conventional technology in the field is adopted, precise instruments and consumables do not need to be used, complex surgical operation does not need to be carried out, operation is easy and convenient, construction is easy, modeling time is short, the liver portal vein ischemia injury model can be constructed within 6 h, and the model is suitable for small animals, high in applicability and low in cost; and meanwhile, the construction method is very stable, and the constructed liver portal vein ischemia injury model is high in repeatability. The method provided by the invention is convenient for carrying out related experimental research on TIPS postoperative complications in the future, is also beneficial to reducing the dosage of drugs and lowering research cost in the research of new drugs, and has good application prospects.

Description

technical field [0001] The invention belongs to the field of animal model construction, and in particular relates to a method for constructing a liver portal vein ischemia injury model. Background technique [0002] Portal hypertension is often secondary to decompensated cirrhosis and is the leading cause of death in patients with cirrhosis. Transjugular intrahepatic portosystemic shunt (TIPS) is a minimally invasive interventional treatment technique for the treatment of complications of portal hypertension such as esophageal and gastric variceal bleeding and intractable ascites. Artificially establish a shunt channel to divert part of the portal vein blood to the hepatic vein, so as to reduce the pressure of the portal vein. [0003] The liver is a solid organ with a dual blood supply, with 25% of the blood supply coming from the hepatic artery and 75% coming from the portal vein. After TIPS, part of the portal vein blood supply is shunted, and the portal vein blood supp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00A01K67/027A61K49/00
CPCA61K49/0008A01K67/027A61D7/00A01K2267/0375A01K2267/035A01K2227/105A01K2207/30
Inventor 王小泽刘国峰刘敬平杨丽
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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