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Extraction solution for extracting vitamins from binding protein and its preparation method and application

A technology for binding proteins and vitamins, which is applied to the extraction liquid and the preparation field of extracting vitamins from binding proteins, can solve the problems of low measurement value of clinical specimens, difficult research and development of quantitative detection reagents, etc., and achieves strong extraction performance, oxidation prevention, high performance stable effect

Active Publication Date: 2022-04-01
GUANGZHOU KEFEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of these three vitamins in human blood are combined with their respective binding proteins. The free state only accounts for a small part, and only the free state can be captured and detected. The reason why these three vitamins bind to the binding protein is The research and development of its quantitative detection reagents has brought difficulties and challenges
Without special treatment, the clinical specimens of these three vitamins generally have low values

Method used

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  • Extraction solution for extracting vitamins from binding protein and its preparation method and application
  • Extraction solution for extracting vitamins from binding protein and its preparation method and application
  • Extraction solution for extracting vitamins from binding protein and its preparation method and application

Examples

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Effect test

Embodiment 1

[0036] An extraction solution for extracting vitamins from binding proteins, using water as a solvent, comprising the following components in terms of mass fraction:

[0037] Carbohydrates 0.15%,

[0038] Metal Chelate 0.1%,

[0039] Surfactant 0.1%,

[0040] Antioxidant 0.2%.

[0041] Wherein, the carbohydrate is 3-mercapto-1,2-propanediol, dithiothreitol and sodium 2-mercaptoethanesulfonate; the metal chelate is EDTA, and the surfactant is Triton X-100 And sodium lauryl sulfate; The antioxidant is 2-mercaptoethanol and ascorbic acid.

[0042] The preparation method of the extract for extracting vitamins in the binding protein is:

[0043] Take 1L of purified water, add 0.05wt% 3-mercapto-1,2-propanediol, 0.05wt% dithiothreitol, 0.05wt% sodium 2-mercaptoethanesulfonate, 0.1wt% EDTA, 0.05wt% Triton X -100, 0.05wt% sodium lauryl sulfate, 0.1wt% 2-mercaptoethanol and 0.1wt% ascorbic acid are mixed evenly and stored at 2-8°C.

Embodiment 2

[0045] An extraction solution for extracting vitamins from binding proteins, using water as a solvent, comprising the following components in terms of mass fraction:

[0046] Carbohydrates 0.45%,

[0047] Metal Chelate 0.1%,

[0048] Surfactant 0.15%,

[0049] Antioxidant 0.2%.

[0050] Wherein, the carbohydrate is 3-mercapto-1,2-propanediol, dithiothreitol and sodium 2-mercaptoethanesulfonate; the metal chelate is EDTA, and the surfactant is Triton X-100 And sodium lauryl sulfate; The antioxidant is 2-mercaptoethanol and ascorbic acid.

[0051] The preparation method of the extract for extracting vitamins in the binding protein is:

[0052] Take 1L of purified water, add 0.15wt% 3-mercapto-1,2-propanediol, 0.15wt% dithiothreitol, 0.15wt% sodium 2-mercaptoethanesulfonate, 0.1wt% EDTA, 0.1wt% Triton X -100, 0.05wt% sodium lauryl sulfate, 0.1wt% 2-mercaptoethanol and 0.1wt% ascorbic acid are mixed evenly and stored at 2-8°C.

Embodiment 3

[0054] An extraction solution for extracting vitamins from binding proteins, using water as a solvent, comprising the following components in terms of mass fraction:

[0055] Carbohydrates 0.75%,

[0056] Metal Chelate 0.1%,

[0057] Surfactant 0.2%,

[0058] Antioxidant 0.2%.

[0059] Wherein, the carbohydrate is 3-mercapto-1,2-propanediol, dithiothreitol and sodium 2-mercaptoethanesulfonate; the metal chelate is EDTA, and the surfactant is Triton X-100 And sodium lauryl sulfate; The antioxidant is 2-mercaptoethanol and ascorbic acid.

[0060]The preparation method of the extract for extracting vitamins in the binding protein is:

[0061] Take 1L of purified water, add 0.25wt% 3-mercapto-1,2-propanediol, 0.25wt% dithiothreitol, 0.25wt% sodium 2-mercaptoethanesulfonate, 0.1wt% EDTA, 0.15wt% Triton X -100, 0.05wt% sodium lauryl sulfate, 0.1wt% 2-mercaptoethanol and 0.1wt% ascorbic acid are mixed evenly and stored at 2-8°C.

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Abstract

The extract for extracting vitamins from binding proteins and its preparation method and application relate to the technical field of vitamin extraction; using water as a solvent, it includes the following components by mass fraction: 0.05% to 0.75% carbohydrates, 0.05% metal chelates ~0.15%, surfactant 0.05~0.25%, antioxidant 0.1~0.5%. The extract of the present invention can effectively extract vitamins (vitamin B9, vitamin B12, vitamin D) from corresponding binding proteins into free states through the cooperation of carbohydrates, metal chelates and surfactants. It also effectively protects free vitamins, prevents oxidation, improves stability, provides good detection conditions for subsequent detection processing, and improves detection accuracy.

Description

technical field [0001] The invention belongs to the technical field of vitamin extraction, and in particular relates to an extract for extracting vitamins from binding proteins, a preparation method and application thereof. Background technique [0002] Vitamin B9 is a compound composed of pteridine, p-aminobenzoic acid and glutamic acid. It is a water-soluble B vitamin. Vitamin B9 has an important nutritional effect on the human body. Deficiency can cause megaloblastic anemia and leukopenia , can also cause physical weakness, irritability, loss of appetite, and psychotic symptoms. In addition, vitamin B9 is especially important for pregnant women. It plays an important role in the growth and development of the early life and the development and maturation of the baby's hematopoietic nervous system. If it is deficient within the first 3 months of pregnancy, it can lead to fetal neural tube development defects, thereby increasing clefts. The incidence of cerebral and anencep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/00G01N1/40C07D475/04C07H1/06C07H23/00C07C401/00
CPCB01D11/00G01N1/4055C07D475/04C07H1/06C07H23/00C07C401/00G01N2001/4061C07C2602/24
Inventor 王敏仪周剑辉
Owner GUANGZHOU KEFEN BIOTECH CO LTD