Peucedanum praeruptorum dunn seedling breeding method

A seedling and Qianhu technology, applied in the breeding field of Qianhu seedlings, can solve problems such as less tissue in Qianhu, achieve the effects of high differentiation rate, increase reproduction coefficient and ensure quality

Pending Publication Date: 2022-02-11
SHANGHAI INST OF TECH
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research report...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The present invention will be further described below in conjunction with specific embodiments, so as to help understand the contents of the present invention.

[0028] The breeding method of a kind of Peucedanum seedling provided by the invention, comprises the following steps:

[0029] Step 1: Selection and processing of explants

[0030] From May to June of that year, new leaves free from diseases and insect pests (about 1 week after leaf expansion) were selected as explants, petioles were removed, soaked with detergent for about 10 minutes, rinsed with running water for 30 minutes, and set aside. On the ultra-clean workbench, first sterilize with 75% alcohol for 30 seconds, then sterilize with 0.1% mercuric solution for 8 minutes, rinse with sterile water for 5 times, then use sterile filter paper to dry the water on the surface of the explants, and remove the explants. The edge part of the blade is then cut into about 0.5-1.0cm2 in size and each explant has veins ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a peucedanum praeruptorum dunn seedling breeding method. The method comprises the following steps: step 1, selection and treatment of explants: selecting fresh leaves without plant diseases and insect pests as the explants, sterilizing with a sterilizing agent, rinsing with sterile water, cutting off the edge parts of the leaves, and cutting the leaves into parts with size of 0.5-1.0 cm < 2 > and with leaf veins; 2, induction of callus: inoculating the explant treated in the step 1 into an induction culture medium, and carrying out dark culture for 50 days to obtain the callus, wherein the culture condition is that the temperature is (25 +/-2) DEG C, and dark culture is carried out; 3, callus proliferation culture: cutting the explant obtained in the step 2 into 0.5 cm < 2 >, inoculating the explant into a proliferation culture medium, and culturing for 30 days to obtain a large number of calluses; and 4, differentiation culture: cutting the callus obtained in the step 3 into 0.5 cm < 2 >, inoculating the callus into a differential culture medium, and culturing for 30 days to obtain a sterile test-tube plantlet, wherein the culture conditions are as follows: the temperature is (25 +/-2) DEG C, the illumination time is 12h/d, and the illumination intensity is 1000-1500LX.

Description

technical field [0001] The invention belongs to the technical field of Peucedanum tissue culture and breeding, and in particular relates to a breeding method of Peucedanum seedlings. Background technique [0002] Peucedanum is a perennial herb of the genus Peucedanum in the family Umbelliferae. The roots are used for medicinal purposes. The medicinal property is bitter, pungent, slightly cold, and returns to the lung meridian. Modern studies have shown that the root of Peucedanum contains a variety of coumarin components, of which keratin-type dihydropyranocoumarins are the main active components, which mainly have various pharmacological effects such as expectorant, antitussive, and asthma. Peucedanum has a medicinal history of thousands of years in my country. Scholars at home and abroad have done a lot of research on the chemical components and pharmacological effects of Peucedanum. Peucedanum is rich in chemical components, and the main components that have been found s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 刘翔王艺程赵玉成秦巧平许瑾孔家烨董竞宇吴晓亭
Owner SHANGHAI INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap