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Cell preparation for treating viral pneumonia

A cell preparation, cell technology, applied in the field of biotechnology and biomedicine

Pending Publication Date: 2022-02-15
BOYALIFE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although there have been reported attempts to use mesenchymal stem cells to treat viral pneumonia, these methods still have their limitations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Embodiment 1, placental whole cell processing:

[0102] 1. Pre-preparation of mixed synthase digestion solution: pipette 22ml of HBSS (Hank's Balanced Salt Solution) containing calcium and magnesium ions, 0.4ml of Roche Liberase MNP-S enzyme (for example, purchased from Xibao Biology, article number: 5578582001), 0.7ml Put ml of DNA type I enzyme in a 50ml centrifuge tube, then add zinc chloride (addition concentration is 0.2g / L, 0.25g / L, or 0.3g / L), mix well, and preheat at 37°C for more than 20min. The Hank's balanced salt solution consists of: 8.0g / L of NaCl, 0.4g / L of KCl, 0.1g / L of MgSO4 7H2O, 0.1g / L of MgCl2 6H2O, 0.06g / L of Na2HPO4 2H2O, 0.06g / L of KH2PO4, 1.0g / L of glucose, 0.14g / L of CaCl2, 0.35g / L of NaHCO3, 0.2g / L of phenol red, hydrochloric acid or sodium hydroxide to adjust the pH to 7.4.

[0103] 2. Preparation of the placenta lobule: take out the placenta from the collection bag and place it in a white porcelain plate, rinse with tissue cleaning solutio...

Embodiment 2

[0118] Embodiment 2, primary cell recovery and subculture

[0119] 1. Cell recovery:

[0120] Take 2 tubes of frozen cells, thaw them quickly at 37°C, transfer the cells to a 15ml centrifuge tube, add 8ml of complete medium to recover by dripping; unless otherwise stated, the complete medium used in this article is DMEM-F12 culture containing 10% FBS base;

[0121] Then centrifuge at 1200rpm for 5min (acceleration 9, deceleration 7), remove the supernatant, add 5ml of complete medium to resuspend; each tube of cells is inoculated in a T75 culture flask, supplement the complete medium to 30ml, and place in a CO2 incubator ( Culture at 37°C, 5% CO2, saturated humidity); replace the medium with complete medium every 3-4 days, count according to the colony formation after 12 days of recovery, until the cell density is not less than 3000 cells / cm 2 Subsequent passages can be carried out;

[0122] 2. Cell subculture: Take the revived P0 generation cells and wash them with PBS, ...

Embodiment 3

[0128] Embodiment 3, biological characteristic identification of placental MSC

[0129] With reference to the method of [0062] to [0089] of the authorized patent CN102676451A (Chinese Patent Application No. 201210044648.X), the biological characteristics of placental mesenchymal stem cells are identified. The results show that the MSCs obtained by applying the method of the present invention have The ability to differentiate into osteoblasts, adipocytes and chondrocytes proves that the MSCs obtained by the method of the present invention have stem cell characteristics. For example, exemplarily, the induction differentiation test is performed on the P5 generation cells, and the results show that these cells have the ability to differentiate into osteoblasts, adipocytes, and chondrocytes. Typical micrographs of adipogenic differentiation, osteogenic differentiation, and chondrogenic differentiation are shown in Figure 6 of CN109481466A.

[0130] In the examples of the prepara...

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PUM

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Abstract

The present invention relates to methods of treating viral pneumonia. Specifically, on one hand, the invention relates to a cell preparation which comprises placenta mesenchymal stem cells and a medicinal solvent, the medicinal solvent is water, the cell preparation is a preparation used in an injection mode, the placenta mesenchymal stem cells are cells subcultured to 1-20 generations, and the concentration of the placenta mesenchymal stem cells is (0.5-10) * 10 <6> cells / ml. In another aspect, the invention relates to the use of said cell preparation for the preparation of a medicament for the treatment of pneumonia, such as viral pneumonia. The cell preparation disclosed by the invention has excellent technical effects as described in the specification.

Description

technical field [0001] The invention belongs to the field of biotechnology and biomedicine, and relates to a method for treating viral pneumonia, in particular to a method for treating viral pneumonia using a cell preparation prepared from mesenchymal stem cells, using the cell preparation prepared from mesenchymal stem cells, and The cell preparation is used for treating viral pneumonia. Background technique [0002] Pneumonia, especially viral pneumonia, is an inflammation of the lungs caused by upper respiratory tract virus infection and downward spread. The disease can occur throughout the year, but most of them are seen in winter and spring, and can be outbreaks or sporadic epidemics. The main clinical manifestations are fever, headache, body aches, dry cough and pulmonary infiltration. The occurrence of viral pneumonia is related to the virulence of the virus, the route of infection, the age of the host, and the state of immune function. Generally, the incidence rat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/00A61K47/02A61P11/00C12N5/0775
CPCA61K35/28C12N5/0668A61P11/00A61K9/0019A61K47/02C12N2509/00
Inventor 刘冰王正汤乐
Owner BOYALIFE
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