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Method for evaluating storage quality of dried sea cucumbers based on iKnife evaporation ionization mass spectrometry

A technology of dried sea cucumber and ionized matter, which is applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of determining time-consuming error, long time-consuming, low accuracy of dry sea cucumber storage quality evaluation, and achieve guaranteed accuracy , improve accuracy, and ensure the effect of cutting length and depth

Pending Publication Date: 2022-02-18
ZHEJIANG GONGSHANG UNIVERSITY +2
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Problems solved by technology

However, it is not accurate enough to reflect lipid oxidation properties at the molecular level
In addition, the determination of traditional indicators is time-consuming and prone to large errors
Therefore, the existing technology has the problems of low accuracy and long time-consuming of dry sea cucumber storage quality assessment.

Method used

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  • Method for evaluating storage quality of dried sea cucumbers based on iKnife evaporation ionization mass spectrometry

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Embodiment Construction

[0020] The present invention will be made in conjunction with the accompanying drawings and examples, but is not discussed as limiting the invention.

[0021] Embodiment. Method of evaluating the quality of dry sea storage based on Iknife evaporative electroplate mass spectrometry, figure 1 As shown, the hand-held electrostrian monopolar cutter is coupled to the REIMS interface and the quadrupole to cut the dry sea sputum by hand-held electrosurgical monolar cutting knife. Ethylene tube and a pneumatic venturi pump with 2BAR nitrogen inhale the quadrupole flight time mass spectrometer, the aerosol is collided with the spiral coil of the collision pool of the quadrupole flight time mass spectrometer, destroy the solvent ion cluster and release gas phase ions. Use the injection pump to 0.2 ng · μL –1 The leucine-enrichum peptide in 2-propanol is injected into the REIMS interface through stainless steel capillary, collecting the original MS data (mass spectrometry data), and sequenti...

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Abstract

The invention discloses a method for evaluating the storage quality of dried sea cucumbers based on iKnife evaporation ionization mass spectrometry, which is characterized in that a handheld electrosurgical monopole cutting knife is combined with an REIMS interface and a quadrupole rod time-of-flight mass spectrometer, and a dried sea cucumber sample to be detected is cut by the handheld electrosurgical monopole cutting knife; the generated aerosol is sucked into a quadrupole time-of-flight mass spectrometer through a polytetrafluoroethylene tube and a pneumatic Venturi pump with 2 bar nitrogen, the aerosol collides with a heated spiral coil in a collision pool of the quadrupole time-of-flight mass spectrometer, a solvent ion cluster is damaged, and gas-phase ions are released; leucine-enkephalin in 0.2 ng.muL<-1> 2-propanol is injected into an REIMS interface through a stainless steel capillary by using an injection pump, original MS data are collected, mass spectrometry and multivariate statistical analysis are sequentially carried out , and the storage quality of the dried sea cucumbers is determined according to an analysis result. The method has characteristics of effectively improving accuracy of dry sea cucumber storage quality evaluation and effectively shortening the evaluation time.

Description

Technical field [0001] The present invention relates to a method of evaluating a dry sea storage quality assessment method, particularly a method of assessing dry sea storage quality based on IKNIFE evaporate electroplate mass spectrometry. Background technique [0002] Stichopus JQPonicus is a marine life in the most underborn marine habitat along the Asian coast. In China and other Asian countries, hundreds of years, sea cucumbers have been used as traditional tonic and nutritional food. Studies have shown that sea cucumbers have anti-cancer effects, anti-inflammatory effects, antioxidant activity, antibacterial properties, biologically active compounds, such as proteins, lipids (such as N-3 and N-6 fatty acids, triglycerides And phospholipids), polysaccharides and minerals. Lipids have unique biological and pharmacological properties, especially the survival and growth of sea cucumber. According to reports, phospholipids rich in tetudic carbonic acid (EPA) in Sea Cucumbers hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 沈清赵巧灵薛静宋恭帅朱小芳王萍亚王宏海
Owner ZHEJIANG GONGSHANG UNIVERSITY
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