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Lysinibacillus fusiformis preparation for degrading polystyrene plastic and preparation of lysinibacillus fusiformis preparation

A technology of Bacillus lysinus and polystyrene plastics, applied in the field of microorganisms, can solve the problems of low degradation efficiency, incomplete degradation of polystyrene plastics, inability to degrade polystyrene microplastics, etc., to protect the ecological environment, The effect of low cost and convenient operation

Pending Publication Date: 2022-02-25
UNIV OF SCI & TECH BEIJING +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] One of the objectives of the present invention is to provide a spindle for biodegrading polystyrene plastics in order to solve the problems of incomplete degradation of polystyrene plastics, low degradation efficiency, and inability to degrade polystyrene microplastics in the prior art. Bacillus lysinica, which can rapidly and efficiently biodegrade polystyrene plastics in aquatic environments

Method used

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  • Lysinibacillus fusiformis preparation for degrading polystyrene plastic and preparation of lysinibacillus fusiformis preparation
  • Lysinibacillus fusiformis preparation for degrading polystyrene plastic and preparation of lysinibacillus fusiformis preparation
  • Lysinibacillus fusiformis preparation for degrading polystyrene plastic and preparation of lysinibacillus fusiformis preparation

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preparation example Construction

[0080] The third aspect of the present invention provides a method for preparing the spindle-shaped lysine bacillus preparation of biodegradable polystyrene plastic as described in the second aspect of the present invention, which includes:

[0081] Step B, inoculating the fermentation strain into the fermentation medium for fermentation culture to obtain a fermentation culture of Bacillus;

[0082] Step C, performing centrifugation on the fermentation culture of Bacillus, harvesting the wet Bacillus cells containing bacteria cells and / or spores as a spindle-shaped lysine bacillus preparation;

[0083] Wherein, the fermented strains are obtained from corresponding bacterial strains through seed cultivation.

[0084] As known to those skilled in the art, at present, 16S rRNA is often used internationally for molecular identification of bacteria, so 16S rRNA can be used for comparison of similarities to obtain their homology. A phylogenetic tree was constructed based on the 16S...

Embodiment 1

[0151] Embodiment 1: Preparation of spindle-shaped lysinyl bacillus liquid preparation

[0152] (1) Preparation of seed solution: Pick a single clone of Lysinobacillus fusiformis PS-02 and inoculate it into 100ml of fermentation liquid medium, culture it on a shaking table at a temperature of 35°C and a rotation speed of 200r / min for 2 days to obtain the fermentation bacteria species (seed solution), in the seed solution, the colony forming unit (CFU) of the strain is 3×10 9 / mL.

[0153] (2) Spindle-shaped lysine Bacillus fermentation culture:

[0154] Under sterile conditions in a clean bench, inoculate 0.2% (v / v) Bacillus spindle-shaped lysine PS-02 bacterial solution in the liquid medium of the Erlenmeyer flask, and the fermentation culture is a shaker fermentation culture in which the strain is free. Fermentation strains are inoculated into the fermentation medium in the form of seed liquid, and the fermentation culture is obtained after batch cultivation at a temperatu...

Embodiment 2

[0158] Example 2: Degradation application of polystyrene microplastics in water

[0159] (1) Degradation treatment

[0160] Add 50mL inorganic salt liquid medium (without carbon source) and polystyrene microplastics (1.0g) with a particle size of 80nm or 100μm after ultraviolet sterilization to a 200mL Erlenmeyer flask to form a composition with polystyrene plastic as the only carbon source Inorganic salt liquid medium. Add 0.5 g of the plastic-degrading bacterial agent produced by fermentation into the Erlenmeyer flask, place it in a shaker at 35° C. and 200 r / min for cultivation, and test the degradation performance after 4 weeks.

[0161] (2) Sample treatment: Stir and wash the collected liquid medium with 2.0% sodium dodecylsulfonate (SDS) aqueous solution for 2 hours to remove the colony attached to the microplastic, wash it with water, and place it in a 65°C refrigerator. Dry in oven.

[0162] (3) Measure the particle size of the sample:

[0163] Particle size analyz...

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Abstract

The invention belongs to the technical field of biology, and relates to a bacillus for biodegradable polystyrene plastic, and the bacillus is a lysinibacillus fusiformis PS-02 strain. The invention also relates to a lysinibacillus fusiformis preparation for degrading the polystyrene plastic, and the lysinibacillus fusiformis preparation contains bacterial cells and / or spores of the bacillus. The bacillus for biodegradable polystyrene plastic provided by the invention can efficiently biodegrade polystyrene plastic films and microplastics, and has an important application prospect in the aspect of biodegradable polystyrene plastic.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a spindle-shaped lysine bacillus preparation for degrading polystyrene plastics and its preparation, in particular to a spindle-shaped lysine bacillus preparation for degrading polystyrene plastics and a preparation method thereof with application. Background technique [0002] The environmental problems caused by plastic products cannot be ignored, and the diffusion and migration of Microplastics (MPs) particles will form, causing various hazards in the global ecosystem. The main types of plastics that form microplastic particle pollutants are polystyrene (Polystyrene, PS), polyethylene (PE) and polypropylene (PP), among which microplastic particles formed by polystyrene have a particularly serious impact on the environment. It is the main microplastic pollutant in the middle and lower Yangtze River Basin and southern mangroves in my country, and accounts for 13% of the tot...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32
CPCC12N1/20C02F3/34C02F2101/32Y02W30/62
Inventor 刘晓璐姚舜禹赵子希万向元闫海汪维俊刘欣洁李金萍
Owner UNIV OF SCI & TECH BEIJING
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