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Microbial agent for improving continuous cropping obstacles in corn planting field through biological method and preparation method of microbial agent

A technology of microbial inoculum and continuous cropping obstacle, which is applied in the field of biological method for improving continuous cropping obstacle of corn planting microbial inoculum and its preparation, and can solve the problems of bacterial species and stable microorganisms, effective colonization and safety restricting the development of biological bacterial fertilizer. , to achieve the effect of inhibiting the growth of soil-borne pathogens, improving soil quality, and alleviating the occurrence of crop diseases

Pending Publication Date: 2022-03-01
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, problems such as the type and stability of bacteria in microbial agents, the effective colonization of microorganisms and safety still restrict the development of biological fertilizers.

Method used

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  • Microbial agent for improving continuous cropping obstacles in corn planting field through biological method and preparation method of microbial agent
  • Microbial agent for improving continuous cropping obstacles in corn planting field through biological method and preparation method of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation method of improving the continuous cropping obstacle microbial bacterial agent by the biological method of corn planting field comprises the following steps:

[0043] Step 1: Preparation of microbial slant seeds: Inoculate Bacillus lateralis sporogenes C1TB-11, Bacillus pumilus C1TB-17 and Pseudomonas putida C1GB-15 on solid bacterial slant medium, and culture at 30°C for 48 hours to obtain Seed slant: Inoculate Penicillium grisea C1TF-24 on the fungal solid slant medium, and cultivate at 30°C for 48 hours to form the seed slant;

[0044] Bacterial solid slant medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, agar 20g, distilled water 1000mL, pH to 7.0, sterilized by high pressure steam at 121°C for 20min;

[0045] Fungal solid slant medium: 200g potato, 20g glucose, 20g agar, 1000ml distilled water, natural pH; wash and peel the potato, weigh 200g and cut into small pieces, add water and boil for 20 minutes, filter with eight layers of gauze,...

Embodiment 2

[0052] The preparation method of improving the continuous cropping obstacle microbial bacterial agent by the biological method of corn planting field comprises the following steps:

[0053] The difference from Example 1 is that the liquid expansion culture of bacterial strains: inoculate Bacillus lateralis C1TB-11, Bacillus pumilus C1TB-17 and Pseudomonas putida C1GB-15 respectively in a 50L fermenter, and the culture medium is bacterial Liquid culture medium, the inoculum amount is 10%, cultivated at a temperature of 30°C and a rotation speed of 200r / min for 36h, until the effective number of viable bacteria in the Bacillus lateralis C1TB-11 bacterial liquid is 5×10 8 cfu / mL, the effective number of viable bacteria in the Bacillus pumilus C1TB-17 bacterial liquid is 5×10 8 cfu / mL, the effective number of viable bacteria of Pseudomonas putida C1GB-15 in the bacterial liquid is 6×10 8 cfu / mL; inoculate Penicillium grisea C1TF-24 in a 50L fermenter, the medium is fungal liquid ...

Embodiment 3

[0055] The preparation method of improving the continuous cropping obstacle microbial bacterial agent by the biological method of corn planting field comprises the following steps:

[0056] The difference from Example 1 is that the bacterial strain liquid expansion culture: inoculate respectively in 50L fermenter with Bacillus lateralis sporogenes C1TB-11, Bacillus pumilus C1TB-17 and Pseudomonas putida C1GB-15, culture medium is Bacterial liquid culture medium, the inoculum amount is 10%, cultivated at a temperature of 30°C and a rotation speed of 200r / min for 40h until the effective number of viable bacteria in the Bacillus lateralis C1TB-11 bacterial liquid is 6×10 8 cfu / mL, the effective number of viable bacteria in the Bacillus pumilus C1TB-17 bacterial liquid is 6×10 8 cfu / mL, the effective number of viable bacteria of Pseudomonas putida C1GB-15 in the bacterial liquid is 7×10 8 cfu / mL; Inoculate Penicillium grisea C1TF-24 in a 50L fermenter, the medium is fungal liquid...

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Abstract

The invention discloses a microbial agent for improving continuous cropping obstacles in a corn planting field through a biological method and a preparation method of the microbial agent. The microbial agent for improving the continuous cropping obstacles in the corn planting field through the biological method comprises the following microbial strains: bacillus laterosporus C1TB-11, bacillus pumilus C1TB-17, pseudomonas putida C1GB-15 and penicillium griseofulvum C1TF-24. The bacillus laterosporus, the bacillus pumilus, the pseudomonas putida and the penicillium griseofulvum are mixed according to the volume ratio of 1: 1: 1: 1; the preparation method comprises the following steps: step 1, preparing microbial slant seeds; step 2, culturing microbial liquid seeds; 3, strain liquid amplification culture; and 4, preparing the microbial agent. By applying the compound microbial agent and adding beneficial microbial florae into continuous cropping soil, the soil microbial community structure is improved, continuous cropping obstacles of the corn are relieved, the soil-borne disease resistance is enhanced, and therefore the purpose of improving the yield and quality of the corn is achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculum, in particular to a microbial inoculum for improving continuous cropping obstacles by biological methods in corn planting land and a preparation method thereof. Background technique [0002] The Hexi region of Gansu has vast land, sufficient sunlight, large temperature difference between day and night, and good natural isolation conditions. It is an ideal seed production base in China. The seed production area accounts for 39% of the country, and the output accounts for 43% of the country. In 1999, it was identified as the best in the country by the Ministry of Agriculture. Seed production area. With the expansion of the corn seed production area, the continuous cropping phenomenon is serious, which leads to serious soil-borne diseases, poor growth and development of corn, and greatly reduces the yield and quality. This has become one of the main obstacles in the production of seed corn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/27A01N63/36A01P3/00C09K17/14C09K101/00C12R1/80C12R1/40C12R1/07
CPCC12N1/20C12N1/14A01N63/22A01N63/27A01N63/36C09K17/14C09K2101/00
Inventor 彭轶楠季彬王治业祁宏山席鹏叶泽杜津昊
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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