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Preparation method and application of nano-selenium modified by astragalus polysaccharide and loaded with tanshinone IIA

A technology of astragalus polysaccharide and tanshinone, which is applied in the directions of nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of low oral bioavailability, slow curative effect of compound traditional Chinese medicine, poor water solubility of traditional Chinese medicine, etc. Damage and repair of nerve damage

Pending Publication Date: 2022-03-04
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional Chinese medicine currently used in clinical practice has a long course of treatment, and the curative effect of compound Chinese medicine is slow. At the same time, most Chinese medicines have relatively poor water solubility and low oral bioavailability. Therefore, the therapeutic effect of traditional Chinese medicine on nerve cell repair needs to be improved urgently.

Method used

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  • Preparation method and application of nano-selenium modified by astragalus polysaccharide and loaded with tanshinone IIA
  • Preparation method and application of nano-selenium modified by astragalus polysaccharide and loaded with tanshinone IIA
  • Preparation method and application of nano-selenium modified by astragalus polysaccharide and loaded with tanshinone IIA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The nano-selenium modified by astragalus polysaccharide and loaded with tanshinone IIA is prepared according to the following method. The nano-selenium is used as a drug carrier to load tanshinone IIA, and the surface is modified with astragalus polysaccharide. The preparation method is as follows:

[0028] Dissolve 1mL of 20mM sodium selenite in a beaker, add 500μL of 20mg / mL astragalus polysaccharide and 125μL of 1mg / mL tanshinone IIA, and finally add 1mL of 80mM ascorbic acid solution drop by drop, shake while adding, and then Pure water was fixed to 5mL. In this system, the final concentration of sodium selenite was 4mM, the final concentration of astragalus polysaccharide was 2mg / mL, and the final concentration of tanshinone IIA was 25μg / mL, then stirred and reacted at room temperature for 4h, and then The reaction mixture was dialyzed with ultra-pure water for 24 hours to prepare nano-selenium modified by astragalus polysaccharide and loaded with tanshinone IIA.

Embodiment 2

[0030] The nano-selenium modified by astragalus polysaccharide and loaded with tanshinone IIA is prepared according to the following method. The nano-selenium is used as a drug carrier to load tanshinone IIA, and the surface is modified with astragalus polysaccharide. The preparation method is as follows:

[0031] Dissolve 2mL of 10mM sodium selenite in a beaker, add 250μL of 40mg / mL astragalus polysaccharide and 125μL of 1mg / mL tanshinone IIA, and finally add 2mL of 40mM ascorbic acid solution drop by drop, shake while adding, and then Pure water was fixed to 5mL. In this system, the final concentration of sodium selenite was 4mM, the final concentration of astragalus polysaccharide was 2mg / mL, and the final concentration of tanshinone IIA was 25μg / mL, then stirred and reacted at room temperature for 5h, and then The reaction mixture was dialyzed with pure water for 26 hours to prepare astragalus polysaccharide-modified nano selenium loaded with tanshinone IIA.

Embodiment 3

[0033] Next, the astragalus polysaccharide-modified nano-selenium loaded with tanshinone IIA obtained by the preparation method in Example 1 was compared with exposed nano-selenium (SeNPs) and astragalus polysaccharide-modified nano-selenium (SeNPs-APS).

[0034] (1) Use transmission electron microscopy to observe three kinds of nano-selenium materials, which are respectively; bare nano-selenium (SeNPs), astragalus polysaccharide-modified nano-selenium (SeNPs-APS), astragalus polysaccharide-modified loaded tanshinone TSIIA Nanoselenium (TSIIA@SeNPs-APS). SeNPs tend to aggregate, while TSIIA@SeNPs-APS is more dispersed. Therefore, the transmission electron microscope showed that nano-selenium was successfully prepared.

[0035] (2) Detection of three kinds of nano-selenium scavenging ABTS + free radical capacity. Such as figure 2 As shown, compared with the other two nanoselenium materials, TSIIA@SeNPs-APS cleared ABTS + The ability of free radicals is the strongest, and ...

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Abstract

The preparation method comprises the following steps: dissolving sodium selenite in a beaker, adding astragalus polysaccharide and tanshinone IIA, finally dropwise adding an ascorbic acid solution, shaking while adding, adding water to a constant volume, carrying out a stirring reaction at room temperature, filtering, washing, and drying to obtain the nano-selenium modified by astragalus polysaccharide and loaded with tanshinone IIA. And dialyzing the reacted mixture with ultrapure water to obtain the nano-selenium modified by the astragalus polysaccharide and loaded with the tanshinone IIA. The astragalus polysaccharide modified tanshinone IIA loaded nano-selenium provided by the invention has excellent oxidation resistance, can effectively scavenge free radicals and reactive oxygen species (ROS), and reverses and improves nerve injury caused by spinal cord injury oxidative stress.

Description

technical field [0001] The application belongs to the field of drug carriers, and in particular relates to a preparation method and application of nano selenium modified by astragalus polysaccharide and loaded with tanshinone IIA. Background technique [0002] Spinal cord injury (SCI) has the characteristics of high mortality rate, high disability rate and difficult rehabilitation, which brings heavy burden to family and society. However, there is still a lack of effective therapeutic measures for nerve regeneration and repair after spinal cord injury. Aiming at its pathological mechanism and exploring exact and effective therapeutic targets has always been a research hotspot at home and abroad. [0003] The pathological changes in the local microenvironment of SCI involve many aspects such as oxidative stress, inflammatory response, accumulation of cytotoxic substances, ion disorder imbalance and blood-spinal cord barrier permeability changes, while the oxidative stress ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/58A61K33/04A61K47/61A61P25/00B82Y5/00B82Y40/00
CPCA61K31/58A61K33/04A61K47/61A61P25/00B82Y5/00B82Y40/00A61K2300/00Y02A50/30
Inventor 陈填烽陈博来饶思远
Owner JINAN UNIVERSITY
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