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Application of isorhamnetin in treatment of influenza

A technology of isorhamnetin and aspect, applied in the application field of isorhamnetin in the treatment of influenza, can solve problems such as unreported, and achieve the effect of improving inflammation and deterioration

Pending Publication Date: 2022-03-11
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Isorhamnetin is a monomeric component of flavonoids. It is known that it has anti-inflammatory effects in various inflammatory diseases such as osteoarthritis, periodontitis and inflammatory bowel disease. However, this product inhibits influenza virus and down-regulates influenza virus Mediated inflammation and the role of modulation of type I interferons in amplifying influenza virus-mediated inflammatory responses has not been reported

Method used

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  • Application of isorhamnetin in treatment of influenza
  • Application of isorhamnetin in treatment of influenza
  • Application of isorhamnetin in treatment of influenza

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isorhamnetin inhibits the replication of influenza virus

[0033] 1. Experimental materials

[0034] Cells: dog kidney cells (Madin Darby Canine Kidney, MDCK) cells

[0035] Virus: Influenza A PR8 strain (A / PR / 8 / 34, H1N1)

[0036] Reagents and drugs: fetal bovine serum (GIBCO, USA); MEM medium (GIBCO, USA); PBS (GIBCO, USA); TPCK trypsin (Sigma, USA); MTT kit (Sigma, USA); Sigma Company); Positive control drug oseltamivir, purchased from Bi De Pharmaceutical Company; isorhamnetin.

[0037] 2. Drug toxicity test (MIT method)

[0038] MDCK cells in the logarithmic growth phase were inoculated into 96-well plates, about 2.5×10 per well 4 After 24 hours, when the cells grow into a single layer, discard the culture medium, add 100 μL / well of drugs at different dilutions, add 100 μL / well of MEM to the blank control and normal cell control wells, and store at 37°C and 5% CO 2 After continuing to cultivate for 36 to 48 hours, add 20 μL of MTT solution (5 mg / mL) t...

Embodiment 2

[0050] Example 2 Isorhamnetin inhibits the inflammatory response caused by influenza virus

[0051] 1. Experimental materials

[0052] Drug: Isorhamnetin.

[0053] Cells and viruses: human lung adenocarcinoma 549 cell line (A549), influenza A H1N1 virus (A / PR / 8 / 34, H1N1)

[0054] Reagents: DMEM medium; PBS and fetal bovine serum were purchased from GIBCO Company; TPCK was purchased from Guangzhou Jabbes Company; Reat-time PCR reagent was purchased from TaKarRa Company, Cat#RR390A; RIPA lysate was purchased from Sigma Company in the United States.

[0055] instrument:

[0056] HERAcell 150i constant temperature CO2 incubator, American Thermo Company;

[0057] BHC-1300IIA / B3 secondary biological safety cabinet, Suzhou Purification Equipment Co., Ltd.;

[0058] Leica DM3000 inverted microscope, Leica Corporation;

[0059] TGL-16M high-speed desktop refrigerated centrifuge, Eppendorf, Germany;

[0060] Avanti J-26 high-speed refrigerated centrifuge, BECKMAN COULTER, USA;

...

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Abstract

The invention relates to the technical field of medicines, in particular to application of isorhamnetin in the aspect of treating influenza. The invention provides a new application of isorhamnetin, namely the isorhamnetin is used for treating influenza, and the isorhamnetin can inhibit the replication of various subtype influenza viruses, has a remarkable curative effect on influenza, and can be applied to the treatment and prevention of influenza; the invention further provides another new application of the isorhamnetin, namely, the isorhamnetin is used for improving the inflammatory reaction caused by the influenza virus, and the isorhamnetin can inhibit abnormal expression of cell inflammatory factors mediated by the influenza virus and can be used for treating the inflammatory reaction caused by the influenza. The invention also provides another new application of the isorhamnetin, namely the isorhamnetin is used for treating influenza virus mediated inflammation amplified by the I-type interferon, so that the problem of inflammation deterioration caused by the I-type interferon is solved.

Description

technical field [0001] This application relates to the field of medical technology, more specifically, relates to the application of isorhamnetin in the treatment of influenza. Background technique [0002] The severity of the disease after influenza virus infection depends on the relative relationship between viral virulence and host resistance, early infection cytokines and chemokines can recruit inflammatory cell aggregation, and increased expression of anti-inflammatory, antiviral and apoptotic genes is accompanied by tissue Immunity to injury and initiation of repair. Type I interferons play an important role in activating the host immune system and amplifying inflammatory responses. However, a strong inflammatory response can lead to the accumulation of a large number of inflammatory cells and inflammatory factors at the infection site, resulting in respiratory mucosal edema, congestion and increased secretions, and eventually death. Therefore, while anti-influenza v...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P31/16A61P11/00
CPCA61K31/352A61P31/16A61P11/00
Inventor 周露茜张清玲李菁徐轶凡林志威关力理
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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