Keratinase-producing stenotrophomonas maltophilia and application thereof
A technology of stenotrophomonas and keratinase, applied in the direction of application, bacteria, and microbial-based methods, can solve the problems of polluting the environment and achieve the effect of rich content and good nutritional value
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Embodiment 1
[0054] Embodiment 1 The keratinase produced by Stenotrophomonas maltophilia (Stenotrophomonas maltophilia) Wu2 provided by the present invention degrades feathers
[0055] This embodiment adopts two groups of comparative experiments, one group is to put feathers into water, and the other group is to put feathers into the fermentation supernatant of fermentation culture; check the degradation results after 5 days, as Figure 4 As shown in A and B, the feathers in A are intact, and the feathers in B are degraded. The test results show that the keratinase degrades 80% of the intact feathers within 5 days, and over 83% of the feathers within 7 days of continued degradation. The degradation time can reach more than 88%.
[0056] The amino acid content in the feather degradation liquid product degraded for 5 days was measured, and the measurement results are shown in Table 2. The total amino acid content exceeds 1100 mg / L, which is rich in amino acids needed for animal growth such a...
Embodiment 2
[0060] Embodiment two to the decontamination application of the keratinase produced by Stenotrophomonas maltophilia Wu2 provided by the present invention
[0061] Place the cotton dripped with 150 μL of blood at 60°C for 30 minutes, and then evaluate the washing performance by measuring the removal of blood stains on the cotton cloth. Carry out following treatment to the cotton cloth that has blood stain: A, put into 100ml tap water. B, put into 100mL of blue moon detergent with a concentration of 0.7mg / mL. C, put into 100mL the fermented supernatant of the fermentation culture containing keratinase of the present invention, keratinase content is 1000U. D, put in 100mL of mixed solution of keratinase and detergent; the blue moon detergent is boiled for 30 minutes before use to inactivate endogenous enzymes, the concentration of keratinase is 100-200U / mL, and the concentration of blue moon detergent is 0.7~1.0mg / mL. The treatment time of the above four groups is 10-20 minute...
Embodiment 3
[0063] Embodiment three, the hair removal application of the keratinase produced by Stenotrophomonas maltophilia Wu2 provided by the present invention
[0064] Stenotrophomonas maltophilia Wu2 keratinase provided by the present invention has only 2.7U / mL to type I collagen protein activity, therefore preliminarily judges that keratinase of the present invention can be used for animal fur depilation, excavates its usefulness in tanning industry application potential. The present embodiment is provided with three tests, A water treatment: sheepskin is placed in clear water at room temperature; B chemical method: the chemical method reported by Sivasubramanian etc. is used as contrast, and mainly adopts lime and sodium sulfide to process sheepskin; C enzymatic method: put The sheepskin (size 6cm*6cm) is placed in the fermentation supernatant containing 20000U activity of keratinase, and incubated at 30-37°C and 200rpm; it is taken out regularly to detect the depilation of the she...
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