Method for preventing and controlling spodoptera frugiperda by using sex pheromone isotropic agent and application
A technology of Spodoptera frugiperda and trapping agent, which is applied in the field of biological control, can solve the problems of ecological environment damage to food quality and safety, affect the survival of natural enemies, and reduce the control effect, and achieve environmental friendliness, long-lasting effect, and simple operation. Effect
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Embodiment 1
[0012] The preparation method of embodiment 1 Spodoptera frugiperda sex pheromone misdirecting agent
[0013] A polyvinyl chloride capillary (PVC) (length 20cm×inner diameter 1.5mm) with sustained release was selected as the carrier. Inject the corn oil solution containing cis-8-tetradecene acetate into the polyvinyl chloride capillary at a dose of 5-15 mg, and add 2% of 2,6-di-tert-butyl-4-methylphenol ( BHT) as a stabilizer, after the end, the two ends of the capillary are heated and sealed to make the sex pheromone tropism lure of Spodoptera frugiperda. Before the test, the lures were placed in a -20°C refrigerator and stored for later use.
Embodiment 2
[0014] Embodiment 2 field biological activity determination test
[0015] Select the lure prepared in Example 1 and hang it in a cornfield where Spodoptera frugiperda is harmful. The trap is selected as a barrel trap, and the trap is 20-30cm higher than the crop. Each of the above lure treatments was set up to repeat 3 times. Table 1 shows the different doses, placement numbers and inhibiting mating rates.
[0016]
[0017]
[0018] The test results show that cis-8-tetradecene acetate has different degrees of inhibitory effects on the mating of Spodoptera frugiperda, and as the dose increases, the inhibition of mating is more obvious. When the dose exceeds 11mg, the number of settings per hectare is When the number is 500, the inhibiting mating rate is greater than 90%. At the same time, when the dose of cis-8-tetradecene acetate was 5 mg, the inhibition rate of mating was higher as the number of cis per hectare increased. Considering the cost, it is recommended that ...
Embodiment 3
[0019] Example 3 Field release rate of Spodoptera frugiperda sex pheromone tropism agent
[0020] Select the lure (5 mg of cis-8-tetradecene acetate) prepared in Example 1, hang it in the field, extract the remaining cis-8-tetradecene acetate in the lure every 15 days, and continue Statistics for 4 months, and quantitative determination by gas chromatography. After 4 months, the content of cis-8-tetradecene acetate was still 1.54mg ( figure 2 ), it shows that the sex pheromone tropism agent of fall armyworm of the present invention has a persistence period greater than 4 months in the field, and can be used for preventing and controlling fall armyworm.
[0021] The sex pheromone tropism agent of the present invention can interfere with the mating communication between the male and female of Spodoptera frugiperda, reduce the mating rate of adults to the greatest extent, reduce the number of reproduction, and play the dual role of interference and trapping. It is an efficient,...
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