High-fat feed for efficiently constructing mouse obesity model and modeling method

Abstract

The invention provides a high-fat feed for efficiently constructing a mouse obesity model. The high-fat feed comprises casein, L-cystine, cellulose, mixed minerals, mixed vitamins, choline bitartrate, glucose, maltodextrin, peanut oil, sesame oil, margarine and milk tea powder. The modeling method of the high-fat feed comprises the following steps that mice are fed with the high-fat feed according to the claim 1 in the first week and the third week respectively, common growth and reproduction feed is fed in the second week and the fourth week respectively, and the mice are fed with the high-fat feed all the time from the fifth week to the nth week till a mouse obesity model is formed. The high-fat feed can improve the obesity causing rate and palatability, the modeling method is short in modeling time, seborrheic unhairing caused by excessive skin lipid secretion of a mouse obesity model can be improved, and obesity caused by excessive energy accumulation is facilitated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-fat feed for efficiently constructing a mouse obesity model and a modeling method. Background technique [0002] The cause of human obesity is the accumulation of fat in the body caused by excessive energy intake. In order to formulate a scientific diagnosis plan for the treatment of obesity diseases, animal models of human diseases are often used in preliminary theoretical research, and most studies use high-fat feed to induce obesity animal models. At present, the common induced obesity models often choose SD rats, Wistar rats, and C57BL / 6J mice. However, the high-fat feed produced by domestic and foreign biological companies is not satisfactory in terms of molding rate and molding time. Using the traditional high-fat feed formula (mainly: lard + basal feed) to construct the rat obesity model requires 8-10 weeks, and the C57BL / 6J mice need 10-12 weeks, the expe...

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Problems solved by technology

Using the traditional high-fat feed formula (mainly: lard + basal feed) to construct the rat obesity model requires 8-10 weeks, and the C57BL / 6J mice need 10-12 weeks, the experiment period is long, and It is difficult to achieve a modeling rate of 80% in model mice. It has the characteristics of long modeling time, low molding rate and low palatability. In terms of appearance, long-term feeding of high-fat feed will lead to excessive secretion of skin fat in mice and form Seborrheic hair loss and other problems

At present, laboratories mostly use a single high-fat feed feeding modeling method to build animal obesity models. Due to the low palatability of high-fat feed itself, and continuous single feeding, it is easy to cause problems such as anorexia in animals, resulting in low food utilization and weight gain. Instability and other issues

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