Microfluidic chip for chemiluminescence immunoassay, analysis system and analysis method

Abstract

The invention provides a microfluidic chip for chemiluminescence immunoassay, which belongs to the technical field of in vitro diagnosis, and comprises a chip substrate, absorbent cotton and a sealing cover plate, the microfluidic chip is provided with a sample adding, uniform mixing and elution area, a capillary microvalve control area and a waste liquid recovery area, the sample adding, uniform mixing and elution area is provided with at least three sealed bubble areas, and the sealed bubble areas are communicated with the capillary microvalve control area. And the capillary microvalve control area is designed into a capillary microvalve structure. The invention also provides an analysis system for chemiluminescence immunoassay, which comprises an automatic pipetting module, a temperature control module, a chip horizontal movement module, an ultrasonic mixing module, an automatic elution module, a substrate filling module, a luminescence detection module and a micro-fluidic chip, the operation steps of pretreatment, reaction and detection of a large-scale chemiluminescence analyzer in a laboratory at present are reduced. The invention further provides an analysis method of chemiluminescence immunoassay, a one-step separation method or a two-step separation method is adopted, the operation is simple, the detection speed is high, the detection sensitivity is high, the cost of the micro-fluidic chip is low, and automation is easy to realize.

Description

Technical field [0001] The present invention belongs to the field of in vitro diagnostic technology, involving a microfluidic chip, analysis system and analysis method of chemiluminescent immunoassay. Background technique [0002] In the field of immunized diagnosis, current major immunological techniques include: 1) colloidal gold regulation; 2) Enzyme-linked immunosorbentation (ELISA); 3) chemiluminescence method. Among them, the colloidal gold technology is to use colloidal gold particles or latex particles as labeling traces, and the nitrogen fibrous membrane is used as a carrier, and when there is antigen in the sample, the antigen can bind to a colloidal gold or latex labeled antibody when there is antigen in the sample. When the chromatography is fixed to the detection band, the antibody is fixed to the detection band, and there is a T line display on the detection tape, which is a naked visualization rapid detection technology, but the detection sensitivity is low, which ...

AI Technical Summary

Problems solved by technology

However, the process of eluting unbound samples currently mainly includes: 1) using active driving (such as mechanical force, centrifugal force, electroosmotic force, etc.) The disadvantage is that the cost of the instrument is high, the action is complicated, and the microvalve switch is inconvenient to integrate with the chip; 2) The fluid control of self-driven (such as capillary force) is matched with the passive valve (such as the hydrophobic interface), and the disadvantage is that the fluid control is not easy. Stable, unable to achieve continuous multiple elution processes

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