Core-shell calcium phosphate polypeptide composite nano vaccine and preparation method thereof
A technology of core-shell calcium phosphate and nano-vaccine, which is applied in the direction of nano-technology, nano-technology, nano-medicine, etc., can solve the problems of stimulating immunogenicity, complicated operation steps, prone to aggregation or aggregation, etc., and can improve the stability of particle dispersion , The preparation method is simple, the effect of good particle stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0033] Such as figure 1 Shown, a kind of preparation method of nucleocapsid calcium phosphate polypeptide composite nano-vaccine comprises the following steps:
[0034] Step 1, mix the antigen polypeptide molecules and calcium salt solution, and place at room temperature for 10min-60min;
[0035] As a preference, the polypeptide is a negatively charged antigen polypeptide in a neutral solution, and as a further preference, the polypeptide is: DDD-VNYIKGFRYELYCLARTARTPLK-DDD SEQ NO 01. It should be noted that the polypeptide sequences here are not exhaustive, as long as they are negatively charged antigen polypeptides in neutral solution, they can be applied to the present invention.
[0036] As a preference, the calcium salt includes: one of calcium chloride, calcium citrate, calcium carbonate, calcium nitrate and calcium lactate.
[0037] Step 2, adding the phosphate aqueous solution to the above polypeptide / calcium salt mixture dropwise, and reacting at room temperature fo...
Embodiment 1
[0050] Example 1, a preparation method of a calcium phosphate polypeptide composite nano-vaccine with a core-shell structure. Specifically prepared by the following steps:
[0051] 1) Prepare 1M CaCl 2 Mother liquor: Weigh 554.9mg CaCl using a microanalytical balance 2 To a 15ml centrifuge tube, add 5ml deionized water to obtain a calcium salt solution S(CaCl 2 ).
[0052] 2) Prepare 5mM Na 2 HPO 4 Mother liquor: Weigh 35.4mg Na using a microanalytical balance 2 HPO 4 In the centrifuge tube of 50ml specification, add 50ml deionized water, obtain the 5mM phosphate solution S(Na 2 HPO 4 ).
[0053] 3) Prepare 1mg / ml P1 polypeptide mother solution: Weigh 1.0mg P1 polypeptide into a 4ml glass vial with a microanalytical balance, add 1ml deionized water to obtain 1mg / ml P1 polypeptide solution S(P1).
[0054] 4) Prepare 1 mg / ml CpG mother solution: Weigh 1.0 mg of CpG polypeptide into a 4 ml glass vial with a microanalytical balance, add 1 ml of deionized water to obtain ...
Embodiment 2
[0056] Embodiment 2. A method for preparing a core-shell structure calcium phosphate polypeptide composite nano-vaccine. Specifically prepared by the following steps:
[0057] 1) Prepare 1M CaCl 2 Mother liquor: Weigh 554.9mg CaCl using a microanalytical balance 2 To a 15ml centrifuge tube, add 5ml deionized water to obtain a calcium salt solution S(CaCl 2 ).
[0058] 2) Prepare 5mM Na 2 HPO 4 Mother liquor: Weigh 35.4mg Na using a microanalytical balance 2 HPO 4 In the centrifuge tube of 50ml specification, add 50ml deionized water, obtain the 5mM phosphate solution S(Na 2 HPO 4 ).
[0059] 3) Prepare 1mg / ml P2 polypeptide mother solution: Weigh 1.0mg P2 polypeptide into a 4ml glass vial with a microanalytical balance, add 1ml deionized water to obtain 1mg / ml P1 polypeptide solution S(P2).
[0060] 4) Prepare 1 mg / ml CpG mother solution: Weigh 1.0 mg of CpG polypeptide into a 4 ml glass vial with a microanalytical balance, add 1 ml of deionized water to obtain 1 mg / ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More