Unlock instant, AI-driven research and patent intelligence for your innovation.

Fishbone peptide as well as preparation method and application thereof

A technology of fish bone peptide and fish bone, applied in the field of fish bone peptide and its preparation, can solve the problems of excessive hydrolysis of collagen peptide, low extraction efficiency, large energy consumption, etc., achieve uniform molecular size, complete fermentation and enzymatic hydrolysis, and reduce production cost effect

Pending Publication Date: 2022-04-12
祥农(北京)食品科技有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using hot pressing to extract collagen peptides has mature technology and simple equipment, but the reaction conditions are severe, the degree of protein hydrolysis in the product is different, and the extraction temperature is high, the reaction is violent, the energy consumption is large, and the extraction efficiency is low; fish bones are extracted by enzymatic hydrolysis Collagen peptides are often selected from papain, flavored protease, and alkaline protease, and adjusted to a suitable pH value for extraction. The extraction conditions are relatively mild, but the optimal extraction conditions for various enzymes are different, resulting in low extraction efficiency. Collagen peptides are often over-hydrolyzed and have a severe bitter taste, which affects further applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fishbone peptide as well as preparation method and application thereof
  • Fishbone peptide as well as preparation method and application thereof
  • Fishbone peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] S1 Grind the thawed fish bones into fish bone paste for later use;

[0060] S2 Add the fish bone paste prepared in S1 to the fermentation extraction tank, then add water and maltose 0.1 times the weight of the fish bone paste, adjust the pH value to 6.5 with citric acid and sodium citrate, start stirring, and heat up to 95 ℃ for insulation to obtain a mixture;

[0061] The mass ratio of the fishbone mud to the water is 100:150;

[0062] S3 lowering the temperature of the mixture prepared in S2 to 36° C., incubating for 20 hours, and inoculating 2 wt % of Bacillus subtilis to obtain a fermentation broth;

[0063] S4 After the fermentation broth in S3 is hydrolyzed, the temperature is raised to 105° C., and the temperature is kept for 1 hour;

[0064] S5 separates the fishbone residue and fishbone broth from the feed liquid obtained in S4, collects the fishbone broth and centrifuges to remove impurities and bone oil, and retains the clear liquid in the middle layer; sep...

Embodiment 2

[0068] Same as Example 1, the difference is that the pH value in S2 is 7.5, the temperature is 105° C., and the mass ratio is 100:240; the temperature is lowered to 30° C. in S3, incubated for 30 hours, and the inoculation amount is 1 wt % of Bacillus subtilis; The temperature is controlled at 60°C, the solid content is concentrated to 50%, and the concentration is stopped;

Embodiment 3

[0070] Same as Example 1, the difference is that the pH value in S2 is 7, the temperature is 100°C, and the mass ratio is 100:400; in S3, the temperature is lowered to 40°C, incubated for 24 hours, and the inoculation amount is 5wt% of Bacillus subtilis; in S6 Concentrate the solid content to 30%, stop concentrating;

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a fishbone peptide as well as a preparation method and application thereof, and belongs to the technical field of fishbone peptide extraction. The extraction method mainly comprises the following steps: S1, unfreezing and crushing raw materials; s2, heating; s3, inoculating bacillus subtilis; s4, fermentation and enzymolysis of the culture solution; s5, inactivating the culture solution; s6, separation and filtration of fishbone residues and fishbone soup; s7, vacuum concentration; s8, vacuum freeze drying; finally, the fishbone peptide is prepared. The method for extracting the fishbone peptide by using the bacillus subtilis has the advantages of low consumption, high yield, thorough fermentation and enzymolysis, low production cost, high production efficiency and capability of realizing continuous and industrial production.

Description

technical field [0001] The invention relates to the technical field of extracting fish bone peptide, in particular to a fish bone peptide and its preparation method and application. Background technique [0002] Fish is rich in nutrients and is an important food source for humans. However, fish bones and fish heads that account for 20%-25% of the overall weight of fish will be produced in the process of fish processing. Fish bones and fish heads are either discarded directly during production, which will be a serious waste and pollute the environment; or they are simply processed for feed or fertilizer, and the added value of the product is low. The main components of fish bone are protein, fat, water and ash (mainly Ca, Fe, Zn, Mg, P and other components), and the content of collagen is very high, which is a high-quality resource for developing fish bone peptides. [0003] The current extraction methods of fish bone peptides generally use hot-press extraction or enzymatic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/34A61K38/01A23L33/18C12R1/125
Inventor 陈旭华柳平
Owner 祥农(北京)食品科技有限公司