Hybrid material based on layered double hydroxide-saccharomyces cerevisiae as well as preparation method and application of hybrid material
A layered bimetallic and hydroxide technology, applied in the interdisciplinary field of materials and biology, can solve the problem of insufficient connection between materials and microorganisms, and achieve the best effect of hydrogen sulfide production
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Embodiment 1
[0039] Chemicals:
[0040] Ferric nitrate nonahydrate (Fe(NO 3 ) 3 9H 2 O), sodium hydroxide (NaOH), sodium thiosulfate pentahydrate (Na 2 S 2 o 3 ·5H 2 O) purchased from Sinopharm Chemical Reagent Co., Ltd. Cobalt nitrate hexahydrate (Co(NO 3 ) 2 ·6H 2 O) Purchased from SigmaAldrich. Saccharomyces cerevisiae is commercial Angel dry yeast. All pharmaceuticals were used without additional purification unless otherwise stated. All the deionized water used in the present invention is subjected to carbon dioxide removal operation, thereby avoiding the influence of carbonate radicals on the intercalation of sulfur-containing anions. The specific process is that deionized water is boiled for 10 minutes, and then collected into a sealed container for later use. All the steps involving the contact of the layered double metal hydroxide with water need to seal the reaction vessel with a plastic film.
[0041] Step (1), the preparation of the cobalt-iron layered double hydr...
Embodiment 2
[0061] Step (1), the preparation of the cobalt-iron layered double hydroxide of thiosulfate intercalation:
[0062] The molar ratio of 3:1.5 is 0.54mmol Co(NO 3 ) 2 ·6H 2 O and 0.27mmol Fe(NO 3 ) 3 9H 2 O was dissolved in 20 mL of deionized water as solution A, and at the same time, the molar ratio was 5:1 i.e. 8 mmol NaOH and 1.6 mmol NaOH 2 S 2 o 3 ·5H 2 O was dissolved in another 20 mL of deionized water to obtain solution B. Then, under the conditions of 65° C. and 400 rpm, the solutions A and B were simultaneously added dropwise to 60 mL of deionized water, ie, C, and the pH value was kept around 10.5. After 10 minutes, the product was collected, and the solid red-brown precipitate was dispersed by high-intensity ultrasound, collected by centrifugation, and washed three times with a mixture of deionized water and ethanol (volume ratio 1:3).
[0063] Step (2), preparation of the hybrid (Y@LDH):
[0064] will be 10 7 CFU yeast and 1.0 mg of thiosulfate-intercala...
Embodiment 3
[0067] Step (1), the preparation of the cobalt-iron layered double hydroxide of thiosulfate intercalation:
[0068] The molar ratio 3:0.75 is 0.64mmol Co(NO 3 ) 2 ·6H 2 O and 0.16mmol Fe(NO 3 ) 3 9H 2 O was dissolved in 20 mL of deionized water as solution A, and at the same time the molar ratio was 5:0.5 i.e. 8.72 mmol NaOH and 0.87 mmol NaOH 2 S 2 o 3 ·5H 2 O was dissolved in another 20 mL of deionized water to obtain solution B. Then, under the conditions of 65° C. and 600 rpm rotation speed, solutions A and B were simultaneously added dropwise to 60 mL of deionized water, namely C, and the pH value was kept around 8.5. After 10 minutes, the product was collected, and the solid red-brown precipitate was dispersed by high-intensity ultrasound, collected by centrifugation, and washed three times with a mixture of deionized water and ethanol (volume ratio 1:3).
[0069] Step (2), preparation of the hybrid (Y@LDH):
[0070] will be 10 7 CFU yeast and 0.5 mg thiosulf...
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