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Related OfMYB1R70 gene for increasing synthesis of volatile organic substances in osmanthus fragrans as well as encoding protein and application of related OfMYB1R70 gene

A volatile organic and substance technology, applied in the field of plant molecular biology, to achieve the effect of improving ornamental traits and genetic quality

Active Publication Date: 2022-04-15
景古环境建设股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is also an important member of the MYB family, and the research on increasing the synthesis of volatile organic substances in osmanthus fragrans in the MYB1R subfamily has not been reported.

Method used

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  • Related OfMYB1R70 gene for increasing synthesis of volatile organic substances in osmanthus fragrans as well as encoding protein and application of related OfMYB1R70 gene
  • Related OfMYB1R70 gene for increasing synthesis of volatile organic substances in osmanthus fragrans as well as encoding protein and application of related OfMYB1R70 gene
  • Related OfMYB1R70 gene for increasing synthesis of volatile organic substances in osmanthus fragrans as well as encoding protein and application of related OfMYB1R70 gene

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] First, build a hypercale vectors of a osmantian OFMYB1R70 gene

[0031] (1) Get the genetic gene

[0032] According to the osmanthus all genome database (http: / / 117.78.20.255 / ), Nanjing Forestry University, HTTP: / 117.78.20.255 / , screened to obtain one gene sequence, compared with the sequence of the mode plant Arabidopsis, and determined that the gene belongs to myb- The RELATED gene family is named OFMYB1R70 based on the position of the genome member in the chromosome.

[0033] (2) Design primers

[0034] The nucleotide full-length sequence of the gene was analyzed by the Bioxm software, and KPNI and SMAI were selected as the restriction endonuclease slit. Design primers using CE Design software. Fill in the relevant information as required, specifically includes sequences near the enzyme dug point of the 1300 vector, the purpose of the target gene, fill in the enzyme docking point in the order of the 5 'end and the 3' end, to obtain an amplified carrier MYB1R70- 1300 pr...

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Abstract

The invention discloses a related OfMYB1R70 gene for increasing synthesis of volatile organic substances in osmanthus fragrans and an encoding protein and application thereof, and belongs to the field of plant molecular biology. The OfMYB1R70 gene is an MYB1R transcription factor gene, and the nucleotide sequence of the OfMYB1R70 gene is as shown in SEQ ID NO. 1. According to the invention, the full length of the gene sequence is obtained through cloning, an over-expression vector is constructed, and instantaneous transformation of the nicotiana benthamiana is carried out. The result shows that the substance content of the main volatile organic compound beta-ionone of the sweet osmanthus of the instantaneous transformation plant is obviously increased compared with that of a control group CK, and the content of other substances is obviously higher than that of the control group CK, so that the OfMYB1R70 plays an important role in increasing the volatile organic substances of the sweet osmanthus; the method can be used for breeding work such as improvement of ornamental characters and genetic quality in sweet osmanthus genetic engineering.

Description

Technical field [0001] The present invention belongs to the field of plant molecular biology, and in particular to an increase in osmanthus volatile organic substance synthesis related OFMYB1R70 genes and their encoded proteins and applications thereof. Background technique [0002] Osmanthus fragrans, Willman Wood is always green broadlet, and it is known as fragrance. Long life is long, widely adapted, widely distributed in multiple cities. Myb-related (referred to as MYB1R) transcription factor family is a class-specific transcription factor family, participating in secondary metabolites synthesis, cytometry regulation, cell expansion, cycle protein transcriptional control, lower embryo axis elongation and blade aging, etc. The process has a very important biological significance for the development of plants. Related studies have shown that the MYB1R transcription factor can regulate the synthesis of secondary metabolites such as anthocyanins and flavonoids. The former people...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/82A01H6/00
Inventor 杨秀莲冯科艺严欣丁卉芬宰舟颖岳远征王良桂
Owner 景古环境建设股份有限公司