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Preparation method of nano starch-based bionic glutathione peroxidase

A technology of glutathione peroxide and nano-starch, which is applied in the field of starch modification, to achieve the effect of expanding the scope of application and expanding the scope of application

Active Publication Date: 2022-04-29
BEIBU GULF UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can load more biologically active substances, and deliver and target biological activities through functional foods. At the same time, selenium-enriched starch belongs to micron-sized particles, so it has certain applications in the absorption and transmission of food and drugs in the body. limitation

Method used

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  • Preparation method of nano starch-based bionic glutathione peroxidase
  • Preparation method of nano starch-based bionic glutathione peroxidase
  • Preparation method of nano starch-based bionic glutathione peroxidase

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Embodiment 1

[0036] A preparation method of nano-starch-based biomimetic glutathione peroxidase, comprising the steps of:

[0037] In the first step, gelatinize the tapioca starch emulsion in a boiling water bath, add pullulanase (the mass ratio of enzyme activity to starch is 50U / g) and enzymolyze it at 60°C for 6h. After the enzymolysis, put the supernatant in 4 Store at ℃ for 12h, then wash and freeze-dry to prepare nano starch.

[0038] In the second step, nano-starch is dissolved in NaOH (0.15M) solution, acetonitrile solution (3g / 10mL) of p-toluenesulfonyl chloride (p-TsCl) is added dropwise, and reacted at 40°C for 6 hours. Make the system pH > 12.5, then alternately wash with ethanol and deionized to neutral, and freeze-dry to prepare SN-starch.

[0039] In the third step, selenium and sodium borohydride were added into 50 mL of deoxygenated deionized water under a nitrogen protective atmosphere, and a sodium selenium hydride (NaSeH) stock solution was prepared at room temperature...

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Abstract

The invention relates to the technical field of starch modification, in particular to a preparation method of nano starch-based bionic glutathione peroxidase. The invention relates to a preparation method of nano starch based bionic glutathione peroxidase. The preparation method comprises the following steps: (1) preparing nano starch; (2) preparation of sulfonated nano starch (SN-start); (3) preparing a sodium hydrogen selenide (NaSeH) stock solution; and (4) adding the SN-start prepared in the step (2) into a mixed solvent system of absolute ethyl alcohol and deionized water, adding the NaSeH stock solution into an SN-start emulsion under the protection of nitrogen, then reacting for a certain time under a proper temperature condition, and washing and freeze-drying after the reaction is finished to prepare a Se-SN-start sample. The Se-SN-start shows an obvious antioxidant catalytic activity advantage compared with similar glutathione peroxidase (GPx), so that the Se-SN-start has more potential in application in the field of selenium-rich functional food development, the application range of modified starch can be expanded, and a new functional material can be provided for antioxidant food and drugs.

Description

technical field [0001] The invention relates to the technical field of starch modification, in particular to a preparation method of nano starch-based biomimetic glutathione peroxidase. Background technique [0002] Glutathione peroxidase (GPx) is a selenium-containing enzyme that can remove excess free radicals in the human body and maintain the metabolic balance of active oxygen in the body. The development and application of GPx is of great significance to the inhibition and treatment of oxidative diseases. However, natural GPx has disadvantages such as limited sources, low stability, high extraction cost, and low yield, which greatly restrict its application. Constructing artificial simulants of GPx and studying related catalytic behaviors and mechanisms are the main means to solve the above-mentioned problems of natural GPx. [0003] Starch is a typical biodegradable natural polysaccharide, and starch nanoparticles produced by nanotechnology process are not harmful to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P19/16C08B31/00
CPCC12P19/04C12P19/16C08B31/00
Inventor 石成胡汉娇尹艳镇梁兴唐张瑞瑞郑韵英
Owner BEIBU GULF UNIV
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