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Lactobacillus rhamnosus with ochratoxin A removing effect

A technology of Lactobacillus rhamnosus and ochratoxin, applied in the field of microorganisms, can solve the problems of limited clearance, low effect, and rarely used food juice, etc., and achieve the effect of wide application prospects and good tolerance

Pending Publication Date: 2022-05-03
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effect of removing ochratoxin A by microorganisms is generally not high, and the removal of ochratoxin A in aqueous solution or medium is limited, and it is rarely used in food juice

Method used

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  • Lactobacillus rhamnosus with ochratoxin A removing effect
  • Lactobacillus rhamnosus with ochratoxin A removing effect
  • Lactobacillus rhamnosus with ochratoxin A removing effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Drawing of the removal efficiency map of ochratoxin A degraded by Lactobacillus rhamnosus CCTCC M 2022013 at different pH.

[0031] 100 μL of activated L. rhamnosus Bm01 strain was inoculated into 5 mL of fresh MRS liquid medium (pH 3, 4, 5, 6 and 7) containing 50 ng / mL ochratoxin A. A medium containing only 50 ng / mL ochratoxin A was used as a control. Each group of samples was cultured in a 37°C incubator for 72 hours before sampling. After the samples were filtered through a 0.22 μm filter membrane, the content of ochratoxin A was determined by high performance liquid chromatography. Three replicates were set for each treatment, and the experiment was repeated twice.

Embodiment 2

[0032] Example 2: Drawing of the removal efficiency diagram of ochratoxin A degraded by Lactobacillus rhamnosus CCTCC M 2022013 at different temperatures.

[0033] 100 μL of activated L. rhamnosus Bm01 strain was inoculated into 5 mL of fresh MRS liquid medium containing 50 nμg / mL ochratoxin A. A medium containing only 50 ng / mL ochratoxin A was used as a control. Each group was cultured in an incubator at 15°C, 25°C and 37°C, respectively, and samples were taken at 0h, 24h, 48h, and 72h. After the samples were filtered through a 0.22 μm filter membrane, the content of patulin was determined by high performance liquid chromatography. Three replicates were set for each treatment, and the experiment was repeated twice.

Embodiment 3

[0034] Example 3: The scavenging effect of Lactobacillus rhamnosus CCTCC M 2022013 on ochratoxin A at different times.

[0035] The activated L. rhamnosus Bm01 strain was inoculated into fresh medium with 2% inoculum amount, and ochratoxin A standard was added to a final concentration of 50 ng / mL, and cultured in a 37°C incubator. Samples were taken at 0 h, 24 h, 48 h, and 72 h. After the samples were filtered through a 0.22 μm filter membrane, the content of ochratoxin A was determined by high performance liquid chromatography. Three replicates were set for each treatment, and the experiment was repeated twice.

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Abstract

The invention relates to lactobacillus rhamnosus capable of eliminating ochratoxin A. The lactobacillus rhamnosus is preserved in the China Center for Type Culture Collection on January 5, 2022, and the preservation number is CCTCC M 2022013. Lactobacillus rhamnosus is activated, cultured and centrifuged to obtain thalli, and the centrifuged thalli are washed and centrifuged to obtain the lactobacillus rhamnosus biological preparation. And resuspending the thalli by using a protective agent until the concentration reaches 1010 CUF / mL, culturing the suspension, and freeze-drying to obtain the lactobacillus rhamnosus powder biological preparation. The lactobacillus rhamnosus (Lactobacillus rhamnosus Bm01) provided by the invention has acid resistance, can be used for rapidly removing the ochratoxin A in an aqueous solution, can be used for remarkably reducing the ochratoxin A in grape juice and coffee, is used for removing the ochratoxin A in food, and has a very wide application prospect.

Description

technical field [0001] The invention relates to a lactobacillus rhamnosus capable of removing ochratoxin A, belonging to the technical field of microorganisms. Background technique [0002] Ochratoxin A is a toxic secondary metabolite of fungi, mainly produced by Aspergillus and Penicillium, which is teratogenic, carcinogenic, mutagenic and other toxic, and has potential hazards to human and animal health. In 2004, the International Agency for Research on Cancer (IARC) classified OTA as a possible human carcinogen, that is, category 2B substances. [0003] Ochratoxin A is widely found in grains and their products, coffee, fruits and other foods, and is an important food and feed pollutant. The pollution and harm of OTA in grapes and their products are considered to be second only to grains. Fruit types with the most OTA contamination. In order to avoid ochratoxin A poisoning, many relevant agencies have issued relevant standards on the limit of ochratoxin A in food. The E...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23L2/84A23F5/16C12R1/225
CPCC12N1/20C12N1/04A23L2/84A23F5/163A23V2400/175
Inventor 郑香峰夏芳平郑凌利李江魏皖宁杨振泉饶胜其高璐
Owner YANGZHOU UNIV
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