Preparation method of lactic acid-producing bacillus coagulans microbial preparation

A technology of Bacillus coagulans and microbial preparations, applied in the field of microorganisms, can solve the problems of inability to form spores, hinder the practical application of expanded production, etc., and achieve the effect of increasing the number of viable bacteria

Pending Publication Date: 2022-05-06
鑫九通科技(武汉)有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Bacillus coagulans can form spores well on a variety of solid media, it cannot form spores in common l

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Example 1

[0027] Pick a ring of bacteria from the freeze-dried seeds of Bacillus coagulans and inoculate it into beef extract peptone agar test tube slant medium, and cultivate at 37°C for 24 hours; then transfer the test tube slant seeds to beef extract peptone agar eggplant bottle slant medium , cultured at 37°C for 24 hours, and after testing, more than 80% of the cells formed spores. Scrape and wash the mature bacteria mud on the eggplant bottle with sterile water, put it into a 250ml triangular flask, shake to make the bacteria even, and the concentration of the bacteria is 2.0×10 9 cfu / ml to form a seed broth.

[0028] The beef extract peptone agar medium described in this example contains peptone 10g / L, beef extract 5g / L, NaCl 15g / L and agar 15g / L, and the pH is 6.8. Before inoculation, it is autoclaved at 121°C for 20min.

[0029] The seed culture liquid is transferred into a 30L seed tank equipped with a first-level liquid medium with an inoculum of 5% to c...

Example Embodiment

[0036] Example 2

[0037] Pick a ring of bacteria from the freeze-dried seeds of Bacillus coagulans and inoculate it into beef extract peptone agar test tube slant medium, and cultivate at 37°C for 24 hours; then transfer the test tube slant seeds to beef extract peptone agar eggplant bottle slant medium , cultured at 37°C for 24 hours, and after testing, more than 80% of the cells formed spores. Scrape and wash the mature bacteria mud on the eggplant bottle with sterile water, put it into a 250ml triangular flask, shake to make the bacteria even, and the concentration of the bacteria is 5.0×10 8 cfu / ml to form a seed broth.

[0038] The beef extract peptone agar medium described in this example contains peptone 9g / L, beef extract 6g / L, NaCl 14g / L and agar 22g / L, and its pH is 7.0, and it is autoclaved at 121°C for 20min before inoculation.

[0039] The seed culture liquid is transferred into a 30L seed tank equipped with a first-level liquid medium with an inoculum of 3% to...

Example Embodiment

[0046] Example 3

[0047] Pick a ring of bacteria from the freeze-dried seeds of Bacillus coagulans and inoculate it into beef extract peptone agar test tube slant medium, and cultivate at 37°C for 24 hours; then transfer the test tube slant seeds to beef extract peptone agar eggplant bottle slant medium , cultured at 37°C for 24 hours, and after testing, more than 80% of the cells formed spores. Scrape and wash the mature bacteria mud on the eggplant bottle with sterile water, put it into a 250ml conical flask, shake to make the bacteria even, and the concentration of the bacteria is 1.0×10 9 cfu / ml to form a seed broth.

[0048] The beef extract peptone agar medium described in this example contains peptone 10g / L, beef extract 5g / L, NaCl 15g / L and agar 15g / L, and its pH is 6.9, and it is autoclaved at 121°C for 20min before inoculation.

[0049] The seed culture liquid is transferred into a 30L seed tank equipped with a first-class liquid medium with an inoculum of 4% to c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of a lactic acid-producing bacillus coagulans microbial preparation, which comprises the following steps: 1) transferring bacillus coagulans of which more than 80% of thalli form spores into sterile water to form a seed culture solution; 2) transferring the seed culture solution into a first-stage liquid culture medium for first-stage fermentation to prepare a first-stage fermentation solution; 3) transferring the primary fermentation liquor into a secondary liquid culture medium for secondary fermentation to prepare secondary fermentation liquor; and (4) inoculating the secondary fermentation liquor into a bran solid culture medium, uniformly mixing, and culturing for 60-72 hours to obtain the lactic acid-producing bacillus coagulans microbial preparation. The invention provides a liquid culture medium and a liquid culture spore production condition suitable for bacillus coagulans, and the prepared lactic acid-producing bacillus coagulans microbial preparation contains 83,000,000 to 1,000,000 viable bacteria per gram, the spore germination rate is not lower than 92 percent, the infectious microbe rate is less than 0.2 percent, and the L-lactic acid content is not lower than 36.5 g/L.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method of a lactic acid coagulating bacillus microbial preparation. Background technique [0002] Microbial preparations are used in animal husbandry because of their functions of regulating intestinal dysfunction, maintaining the balance of intestinal flora, improving the health level and production performance of the body, and avoiding drug resistance and double infection caused by taking antibiotics. Has been widely recognized by the industry. However, currently common domestic microbial preparations such as lactic acid bacteria and bacillus are often difficult to combine the dual advantages of excellent production performance and strong stress resistance, which limits the practical application of such additives. [0003] The advantage of Bacillus coagulans is that it can form spores with strong heat resistance and acid resistance. As an activ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 江永明吴峰
Owner 鑫九通科技(武汉)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap