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Preparation method of lactic acid-producing bacillus coagulans microbial preparation

A technology of Bacillus coagulans and microbial preparations, applied in the field of microorganisms, can solve the problems of inability to form spores, hinder the practical application of expanded production, etc., and achieve the effect of increasing the number of viable bacteria

Pending Publication Date: 2022-05-06
鑫九通科技(武汉)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Bacillus coagulans can form spores well on a variety of solid media, it cannot form spores in common liquid media, and the limitations of solid culture will hinder its expanded production and practical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Pick a ring of bacteria from the freeze-dried seeds of Bacillus coagulans and inoculate it into the slant medium of beef extract peptone agar test tube, and cultivate it at 37°C for 24 hours; then transfer the slant seeds of the test tube to the slant medium of beef extract peptone agar eggplant bottle In the medium, cultured at 37°C for 24 hours, after testing, more than 80% of the bacteria formed spores. Scrape and wash the mature fungus slime on the eggplant bottle with sterile water, put it into a 250ml triangular flask, oscillate to make the bacteria uniform, and the bacteria concentration is 2.0×10 9 cfu / ml to form a seed culture solution.

[0028] The beef extract-peptone agar medium described in this example contains 10 g / L peptone, 5 g / L beef extract, 15 g / L NaC and 15 g / L agar, and has a pH of 6.8.

[0029] Transfer the seed culture solution with 5% inoculum amount into a 30L seed tank equipped with a first-level liquid medium for fermentation and cultivation...

Embodiment 2

[0037] Pick a ring of bacteria from the freeze-dried seeds of Bacillus coagulans and inoculate it into the slant medium of beef extract peptone agar test tube, and cultivate it at 37°C for 24 hours; then transfer the slant seeds of the test tube to the slant medium of beef extract peptone agar eggplant bottle In the medium, cultured at 37°C for 24 hours, after testing, more than 80% of the bacteria formed spores. Scrape and wash the mature fungus slime on the eggplant bottle with sterile water, put it into a 250ml triangular flask, oscillate to make the bacteria uniform, and the bacteria concentration is 5.0×10 8 cfu / ml to form a seed culture solution.

[0038] The beef extract-peptone agar medium described in this example contains peptone 9g / L, beef extract 6g / L, NaCl 14g / L and agar 22g / L, and its pH is 7.0, and it is autoclaved at 121°C for 20min before inoculation.

[0039] Transfer the seed culture liquid with 3% inoculum amount into a 30L seed tank equipped with a first-...

Embodiment 3

[0047] Pick a ring of bacteria from the freeze-dried seeds of Bacillus coagulans and inoculate it into the slant medium of beef extract peptone agar test tube, and cultivate it at 37°C for 24 hours; then transfer the slant seeds of the test tube to the slant medium of beef extract peptone agar eggplant bottle In the medium, cultured at 37°C for 24 hours, after testing, more than 80% of the bacteria formed spores. Scrape and wash the mature fungus slime on the eggplant bottle with sterile water, put it into a 250ml triangular flask, oscillate to make the bacteria uniform, and the bacteria concentration is 1.0×10 9 cfu / ml to form a seed culture solution.

[0048] The beef extract-peptone agar medium described in this example contains 10 g / L peptone, 5 g / L beef extract, 15 g / L NaC and 15 g / L agar, and its pH is 6.9.

[0049] Transfer the seed culture liquid with 4% inoculum amount into a 30L seed tank equipped with a first-level liquid medium for fermentation and cultivation, th...

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PUM

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Abstract

The invention relates to a preparation method of a lactic acid-producing bacillus coagulans microbial preparation, which comprises the following steps: 1) transferring bacillus coagulans of which more than 80% of thalli form spores into sterile water to form a seed culture solution; 2) transferring the seed culture solution into a first-stage liquid culture medium for first-stage fermentation to prepare a first-stage fermentation solution; 3) transferring the primary fermentation liquor into a secondary liquid culture medium for secondary fermentation to prepare secondary fermentation liquor; and (4) inoculating the secondary fermentation liquor into a bran solid culture medium, uniformly mixing, and culturing for 60-72 hours to obtain the lactic acid-producing bacillus coagulans microbial preparation. The invention provides a liquid culture medium and a liquid culture spore production condition suitable for bacillus coagulans, and the prepared lactic acid-producing bacillus coagulans microbial preparation contains 83,000,000 to 1,000,000 viable bacteria per gram, the spore germination rate is not lower than 92 percent, the infectious microbe rate is less than 0.2 percent, and the L-lactic acid content is not lower than 36.5 g / L.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method of a lactic acid coagulating bacillus microbial preparation. Background technique [0002] Microbial preparations are used in animal husbandry because of their functions of regulating intestinal dysfunction, maintaining the balance of intestinal flora, improving the health level and production performance of the body, and avoiding drug resistance and double infection caused by taking antibiotics. Has been widely recognized by the industry. However, currently common domestic microbial preparations such as lactic acid bacteria and bacillus are often difficult to combine the dual advantages of excellent production performance and strong stress resistance, which limits the practical application of such additives. [0003] The advantage of Bacillus coagulans is that it can form spores with strong heat resistance and acid resistance. As an activ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 江永明吴峰
Owner 鑫九通科技(武汉)有限公司
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