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Method for detecting nitrofuran metabolites in aquatic products and application

A nitrofuran and detection method technology, applied in the field of nitrofuran metabolite detection, can solve the problem of affecting the detection limit, increase the difficulty of nitrofuran metabolite extraction and qualitative and quantitative, and affect the organic liquid-liquid extraction effect. Phase recovery and other problems, to achieve the effect of wide application, good accuracy and high sensitivity

Active Publication Date: 2022-05-13
唐山市食品药品综合检验检测中心(唐山市农产品质量安全检验检测中心唐山市检验检测研究院)
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Problems solved by technology

[0005] However, due to the high-performance liquid chromatography tandem triple quadrupole mass spectrometry detection method, due to the fact that aquatic products contain more oil in the pretreatment process, the unique matrix of aquatic products will form gel-like substances with ethyl acetate, thereby affecting the liquid quality. - The liquid extraction effect and the recovery of the organic phase increase the difficulty in the extraction and qualitative quantification of nitrofuran metabolites in the sample pretreatment process, and affect their detection limit

Method used

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  • Method for detecting nitrofuran metabolites in aquatic products and application
  • Method for detecting nitrofuran metabolites in aquatic products and application
  • Method for detecting nitrofuran metabolites in aquatic products and application

Examples

Experimental program
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Effect test

preparation example 1

[0051] A preparation method for an aquatic product spiked sample, comprising the following steps:

[0052] Take 50 μL of 100ng / mL nitrofuran standard solution and add it to 5g of shrimp meat muscle tissue, and mix well to obtain a spiked sample; wherein, the nitrofuran standard solution is 3-amino-2-oxazolidinone (AOZ) solution.

preparation example 2

[0054] A preparation method for an aquatic product spiked sample, comprising the following steps:

[0055] Take 100 μL of nitrofuran standard solution of 100 ng / mL and add it to 5 g of shrimp muscle tissue, mix well to obtain the spiked sample;

[0056] Wherein, the nitrofuran standard solution is 3-amino-2-oxazolidinone (AOZ) solution.

preparation example 3

[0058] A preparation method for an aquatic product spiked sample, comprising the following steps:

[0059] Take 250 μL of nitrofuran standard solution of 100 ng / mL and add it to 5 g of shrimp muscle tissue, mix well to obtain the spiked sample;

[0060] Wherein, the nitrofuran standard solution is 3-amino-2-oxazolidinone (AOZ) solution.

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Abstract

The invention relates to the technical field of detection of nitrofuran metabolites, and particularly discloses a method for detecting nitrofuran metabolites in aquatic products. The detection method comprises the following steps: sample pretreatment: taking a water product, adding a standard sample, adding an internal standard working solution and hydrochloric acid, uniformly mixing, adding a derivatization reagent, carrying out a derivatization reaction, sequentially adding ethyl acetate and a neutral strong electrolyte after the reaction is completed, oscillating, and carrying out sample pretreatment; centrifuging, collecting an organic phase, blow-drying with inert gas, redissolving, and filtering to obtain on-machine to-be-detected liquid; the derivatization reagent is a benzaldehyde substance with carboxyl. The detection method of the nitrofuran metabolites can be used for detecting the nitrofuran metabolites in aquatic products, and has the advantages of high accuracy, good sensitivity and simple and convenient operation steps.

Description

technical field [0001] This application relates to the technical field of detection of nitrofuran metabolites, more specifically, it relates to a detection method and application of nitrofuran metabolites in aquatic products. Background technique [0002] Nitrofuran drugs are synthetic broad-spectrum antibacterial drugs, which are effective against most Gram-positive bacteria and Gram-negative bacteria, some fungi and protozoa, and are used to treat and prevent infections caused by bacteria or protozoa. Various gastrointestinal infections. [0003] Nitrofuran drugs may be illegally used in the breeding process of aquatic products. Common such drugs mainly include furazolidone and nitrofurantoin. After nitrofuran drugs are used, they will be rapidly metabolized in aquatic products, and their metabolites can be combined with tissue proteins to form "protein-bound metabolites", which always exist throughout the breeding process. Nitrofuran metabolites have been proven to have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/062G01N2030/067
Inventor 周鑫马瑞欣项爱丽张书宏郑百芹侯蔷段晓然张谊汤思凝齐彪郝立武苑中策李艺霍露曼王雪松庞学良阴明杰
Owner 唐山市食品药品综合检验检测中心(唐山市农产品质量安全检验检测中心唐山市检验检测研究院)
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