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Analysis method of beta-nicotinamide mononucleotide

An analytical method and single nucleotide technology, applied in the field of detection of beta-nicotinamide mononucleotide, can solve the problem of difficult to achieve effective separation of beta-nicotinamide mononucleotide and impurities, and achieve high sensitivity and exclusive Strong performance and clear peak shape

Pending Publication Date: 2022-05-13
BEIJING HONGHUI MEDITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The main purpose of the present invention is to provide an analytical method for β-nicotinamide mononucleotide, so as to solve the problem in the prior art that it is difficult to effectively separate β-nicotinamide mononucleotide and impurities by using HPLC analysis method

Method used

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  • Analysis method of beta-nicotinamide mononucleotide
  • Analysis method of beta-nicotinamide mononucleotide
  • Analysis method of beta-nicotinamide mononucleotide

Examples

Experimental program
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Embodiment 1

[0051] Take about 10mg of nicotinamide, weigh it accurately, put it in a 10mL volumetric flask, add 0.5mL water to dissolve it, then add acetonitrile-water (4:1 volume ratio) to dilute to the mark, shake well, and make each 1mL contains Niacinamide Stock solution of nicotinamide approximately 1 mg. Take about 10mg of nicotinamide ribose, weigh it accurately, put it in a 10mL volumetric flask, add 0.5mL water to dissolve it, then add acetonitrile-water (4:1 by volume) to dilute to the mark, shake well, and make 1mL Nicotinamide riboside stock solution containing approximately 1 mg of nicotinamide riboside. Precisely measure 5mL of nicotinamide stock solution and 1mL of nicotinamide ribose stock solution respectively, place them in a 100mL volumetric flask, add acetonitrile-water (volume ratio: 4:1) to dilute to the mark, shake well, and make each 1mL contains tobacco A mixed impurity stock solution of amide 50 μg and nicotinamide riboside 10 μg. Take about 10mg of β-nicotinam...

Embodiment 2

[0055] The difference between embodiment 2 and embodiment 1 is that

[0056] The detection wavelength of HPLC analysis is 254nm, and finally the peak shapes of β-nicotinamide mononucleotide, nicotinamide and nicotinamide riboside are clear, and they are well separated from each other.

Embodiment 3

[0058] The difference between embodiment 3 and embodiment 1 is that

[0059] The detection wavelength of HPLC analysis is 270nm, and finally the peak shapes of β-nicotinamide mononucleotide, nicotinamide and nicotinamide riboside are clear, and they are well separated from each other.

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Abstract

The invention provides an analysis method of beta-nicotinamide mononucleotide. The analysis method comprises the steps that HPLC analysis is conducted on a to-be-tested object containing beta-nicotinamide mononucleotide, a Waters XBridge BEH Amide chromatographic column serves as a chromatographic column for HPLC analysis, a mobile phase comprises acetonitrile and a buffer solution, and the pH value of the buffer solution ranges from 3.0 to 10.0. According to the present invention, the Waters XBridge BEH Amide chromatographic column is adopted for the first time to perform gradient elution analysis on the to-be-tested substance of the highly polar beta-nicotinamide mononucleotide, such that the peak appearance time of the beta-nicotinamide mononucleotide is delayed so as to effectively separate the beta-nicotinamide mononucleotide from various impurities. The peak shape of the beta-nicotinamide mononucleotide in the chromatogram obtained by the method is clear, the reproducibility is good, and the method has the characteristics of good separation degree, simplicity, rapidness, strong specificity, high sensitivity and the like.

Description

technical field [0001] The invention relates to the technical field of detection of β-nicotinamide mononucleotide, in particular to an analysis method of β-nicotinamide mononucleotide. Background technique [0002] The molecular formula of β-nicotinamide mononucleotide is C 11 h 15 N 2 o 8 P, the chemical structural formula is as follows: [0003] [0004] β-nicotinamide mononucleotide is the product of nicotinamide phosphoribosyltransferase reaction and also NAD + one of the key precursors. NAD + It is an electron transporter in all biological species, widely exists in various organisms, and plays an extremely important role in life activities. Nicotinamide phosphoribosyltransferase is involved in the intracellular adenylation of β-nicotinamide mononucleotide to synthesize NAD + , and then meet the requirements of NAD in the energy transfer, substance metabolism and signal transduction of living organisms + demand. β-nicotinamide mononucleotide can reduce the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 卢蕾李巧峰张国燕蒋露吕久安童卫民
Owner BEIJING HONGHUI MEDITECH CO LTD
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