Microbial marker and application thereof in diagnosis of male urinary calculi

A marker and microorganism technology, applied in the field of microorganisms, can solve the problems of unpredictable diagnostic schemes for high-risk groups of urinary tract stones, and achieve the effects of simple diagnosis, high accuracy and good detection specificity

Pending Publication Date: 2022-05-17
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since imaging can only identify stones of a certain size, there is currently no clinical diagnosis plan for predicting high-risk groups of urinary tract stones, and early prevention cannot be carried out for high-risk groups, especially male groups

Method used

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  • Microbial marker and application thereof in diagnosis of male urinary calculi
  • Microbial marker and application thereof in diagnosis of male urinary calculi
  • Microbial marker and application thereof in diagnosis of male urinary calculi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, microecological analysis of urinary tract flora

[0028] (1) Urinary tract microbial DNA extraction

[0029] In the present invention, 20 cases of normal males and 20 cases of male urinary tract calculus patients are used as experimental objects, and the clean mid-morning urine samples are collected for three consecutive days, 50 mL each time. The obtained samples were immediately transported and stored in a -80°C low-temperature refrigerator, and then thawed before microbial DNA extraction.

[0030] The thawed urine sample was centrifuged at 16,000×g for 15 minutes at room temperature. After discarding the supernatant, the precipitate was resuspended and washed in 1 mL of normal saline, and centrifuged again at 16,000×g for 5 minutes at room temperature. After repeated washing with normal saline for 3 times, the resulting precipitate was extracted with the total DNA of urine microorganisms using QIAamp PowerFecal DNA Kit (Qiagen, Germany).

[0031] (2) ...

Embodiment 2

[0035] Example 2. Screening of urinary tract characteristic flora mining of male patients with urinary calculi

[0036] 1) Establishment of the study cohort

[0037]In this study, clean morning urine samples from 20 male patients with urinary stones were collected to analyze the urinary tract microecological characteristics of male patients with urinary stones. The inclusion criteria for patients with stones were: (1) age ≥ 18 years; (2) urinary tract ultrasonography, CT or MRI examination within 2 weeks before taking urine samples to confirm the existence of urinary tract stones; (3) Urine routine and urine culture examinations were performed one day before sample collection to confirm that there was no concurrent urinary tract infection; (4) no previous congenital urinary tract abnormalities; (5) to rule out the possibility of secondary urinary tract stones, such as parathyroid Kang et al.; (6) No malignant tumor; (6) No history of systemic or local antibiotic exposure with...

Embodiment 3

[0042] Example 3. Evaluation of diagnostic efficacy of urinary tract characteristic microbial markers in predicting male urinary tract stones

[0043] (1) Collection and detection of a validation cohort of male patients with urinary calculi

[0044] According to the scheme of embodiment 2, 52 male urinary tract microecological samples were collected, and all patients were judged to have calculi by urinary tract imaging examination (one or more examinations in B-ultrasound, CT or MRI), and 32 cases were examined by Imaging examination showed urinary tract calculi, and 20 cases had no urinary tract calculi by imaging examination.

[0045] (2) Evaluation of the diagnostic efficacy of urinary tract microbial markers in judging male urinary tract stones

[0046] Receiver operating characteristic curve (ROC) was used to detect the diagnostic performance of urinary tract microbial markers in judging male urinary calculi. The analysis found that the rising Bacteroidales, Campylobact...

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a microbial marker and application thereof in diagnosis of male urinary calculi. The invention relates to a microbial marker, which is characterized in that the microbial marker comprises bacillus, Bifidobacteria, Campylobacteria, Enterobacteria, Neisseria and Pasteurella, and the microbial marker comprises bacillus, Bifidobacteria, Campylobacteria, Enterobacteria, Neisseria and Pasteurella, and the microbial marker comprises bacillus, Bifidobacteria, Campylobacteria, Enterobacteria, Neisseria and Pasteurella. According to the invention, by detecting the relationship between the male urinary tract microbial phenotype and the urinary tract calculus attack and establishing and applying the urinary tract microecology to assist the diagnosis of the urinary tract calculus, high-risk groups suffering from the urinary tract calculus attack can be screened, and a new means is provided for accurate prevention and control of the urinary tract calculus.

Description

technical field [0001] The invention relates to the technical field of microorganisms and the technical field of medicine, in particular to a microbial marker and its application in diagnosing male urinary tract stones. Background technique [0002] Urolithiasis refers to the formation of particles or block-like aggregates in the urinary system due to water-insoluble precipitation in urine, including kidney stones, ureteral stones, bladder stones, and urethral stones. Urinary tract calculus is a common urinary tract disease. Severe urinary tract calculi can cause severe lower back pain or abdominal pain, hematuria, secondary infection, and impaired kidney function. At present, the cause of urinary stones is still unclear, and its incidence varies significantly in different regions. Studies have pointed out that there are obvious gender differences in the incidence of urinary tract stones, and the prevalence of urinary tract stones in men is significantly higher than that in...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12Q1/689C12N15/11A61K45/00A61P13/04A23L33/135G16B30/10G16B50/30G16B20/00G16B25/00G16B25/20G16H50/30
CPCC12Q1/06C12Q1/689A61K45/00A61P13/04A23L33/135G16B30/10G16B50/30G16B20/00G16B25/00G16B25/20G16H50/30G01N2800/345G01N2800/50A23V2002/00A23V2200/30
Inventor 莫承强谢云关键烽吴荣佩
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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