Application of pomegranate rind tannin in preparation of medicine for treating enterotoxigenic escherichia coli intestinal diseases
A technology of pomegranate tannin and Escherichia coli, which is applied in the direction of antibacterial drugs, drug combinations, and resistance to vector-borne diseases, can solve the problems of unclear growth, obstruction, toxicity and side effects of pomegranate tannin, and achieve maintenance Effects of intestinal homeostasis, promotion of secretion, and increase of expression level
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Embodiment 1
[0041] Example 1: Extraction and enrichment of pomegranate peel tannins
[0042] The extraction and preparation of pomegranate peel tannins are completed by the following steps:
[0043] (1) Sample preparation:
[0044] Fully crush the pomegranate peel with a crusher;
[0045] (2) Extract:
[0046] Weigh the pomegranate peel powder obtained in step (1), add 60% ethanol solution by volume according to the material-to-liquid ratio of 1g: 25mL, soak for more than 2h, then place it in an ultrasonic instrument, ultrasonic-assisted extraction for 50min, and collect after suction filtration The filtrate was repeated 3 times; the filtrate was combined, concentrated under reduced pressure in a rotary evaporator, and then 3% gelatin solution by volume was added until no precipitate was produced, filtered, and the precipitate was collected;
[0047] (3) Enrichment:
[0048] Collect the precipitate obtained in step (2), add 60% acetone solution by volume percentage according to the ma...
Embodiment 2
[0049] Example 2: Application of pomegranate peel tannin in the treatment of intestinal damage in mice induced by enterotoxogenic Escherichia coli
[0050] We first established a mouse model of enterotoxigenic Escherichia coli (ETEC) infection. The experimental design was as follows figure 1 .
[0051] Pomegranate Peel Tannins (PPT) was used to treat enterotoxin-producing Escherichia coli-induced intestinal injury model in mice. The specific steps are as follows:
[0052] 1) Take 6-week-old C57BL / 6 mice and randomly divide them into 4 treatment groups. The treatment groups are the Ctr group, the PPT group, the ETEC group, and the ETEC+PPT group. The number of mice in each treatment group and the treatment methods are as follows:
[0053] Control group (n=6): gavage 100 μL PBS every day;
[0054] PPT group (n=6): 100 μL of pomegranate peel tannin obtained in Example 1 with a concentration of 200 mg / mL was administered orally every day, so that the final dose of pomegranate pe...
Embodiment 3
[0081] Example 3: Application of pomegranate peel tannin in the treatment of intestinal damage of piglets induced by enterotoxogenic Escherichia coli
[0082] We first established a model of piglet intestinal injury induced by enterotoxin-producing Escherichia coli by producing pomegranate peel tannins. The specific steps are as follows:
[0083] 1) Select healthy (21 ± 2) day-old "Du × Chang × Da" weaned piglets with an average body weight of (7.48 ± 0.37) kg, and randomly divide them into 4 treatment groups according to the principle of similar body weight. The treatment groups are the Ctr group and the PPT group. Group, ETEC group, ETEC+PPT group, the number of piglets in each treatment group and the treatment methods are as follows:
[0084] Ctr group (n=6): fed the basal diet, and the piglets were given the same dose of sterile culture medium;
[0085] PPT group (n=6): the experimental diet with 1000g / t pomegranate peel tannin obtained in Example 1 added to the basal die...
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