Transcription factor VcMYB108 related to plant drought tolerance as well as coding gene and application of transcription factor VcMYB108

A plant and coding technology, which is applied to the transcription factor VcMYB108 and its coding gene and application fields, can solve the problem that gene resources need to be tapped and the like

Pending Publication Date: 2022-05-31
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are currently a large number of relevant studies on plant drought tolerance, the genetic resources related to drought tolerance in blueberries still need to be tapped

Method used

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  • Transcription factor VcMYB108 related to plant drought tolerance as well as coding gene and application of transcription factor VcMYB108
  • Transcription factor VcMYB108 related to plant drought tolerance as well as coding gene and application of transcription factor VcMYB108
  • Transcription factor VcMYB108 related to plant drought tolerance as well as coding gene and application of transcription factor VcMYB108

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] The present embodiment carries out the acquisition of VcMYB108 and its encoding gene, including the following steps:

[0078] In this experiment, after screening the transcriptome data of blueberry response to drought, the coding region sequence of blueberry VcMYB108 gene was obtained. Using 5'-ATGGAAGTATATGAAAGCAGAGGTAA-3' and 5'-TTACATGCTGTTAAGCTGCTGCTGTA-3' as primers, the cDNA extracted from blueberry was used as primers. As a template, the open reading frame sequence of VcMYB108 was cloned, ligated into the pEASY-T1 vector and sequenced.

[0079] The sequencing results show that: the nucleotide sequence of the cDNA is SEQ ID NO. 1 in the sequence table, which consists of 948 nucleotides. The gene shown by it is named VcMYB108, the protein encoded by VcMYB108 is named VcMYB108, and its amino acid sequence is SEQ ID NO. 2 in the list consists of 315 amino acids.

Embodiment 2

[0081] The present embodiment analyzes the tissue of VcMYB108 gene and its expression under stress by fluorescence quantitative PCR, including the following steps:

[0082] RNA was extracted from the tissues of blueberry flowers, roots, stems, leaves, fruits and seeds, and cDNA was synthesized by reverse transcription using a reverse transcription kit; and 5'-CTGCTGTAAGAACCAAATGTCCTCA-3' as RT primers for PCR amplification to detect the expression of VcMYB108 gene in different tissues of blueberry. At the same time, using the blueberry VcUBC28 gene as the internal reference gene, the forward primer for amplifying the VcUBC28 gene is: 5'-CCATCCACTTCCCTCCAGATTATCCAT-3', and the reverse primer is: 5'-ACAGATTGAGAGCAGCACCTTGGA-3'.

[0083] The blueberry seedlings with relatively consistent growth were selected and transplanted into plastic pots, with 3 plants in each pot, for a total of 30 pots, and subjected to drought treatment. The relative water content was determined by weigh...

Embodiment 3

[0087] This example verifies the role of VcMYB108 gene in regulating plant drought tolerance.

[0088] In the present invention, the function of the VcMYB108 gene is analyzed and verified by using the model plant Arabidopsis thaliana as a material.

[0089] 1. Acquisition of VcMYB108 transgenic Arabidopsis

[0090] 1. Acquisition of VcMYB108 gene

[0091] In this experiment, 5'-ATGGAAGTATATGAAAGCAGAGGTAA-3' and 5'-TTACATGCTGTTAAGCTGCTGCTGTA-3' were used as primers, and the cDNA extracted from blueberry was used as a template to clone the open reading frame sequence of VcMYB108, which was connected to the pEASY-T1 vector and subjected to further analysis. Sequencing.

[0092] The sequencing results show that: the nucleotide sequence of the cDNA is SEQ ID NO. 1 in the sequence table, which consists of 948 nucleotides. The gene shown by it is named VcMYB108, the protein encoded by VcMYB108 is named VcMYB108, and its amino acid sequence is SEQ ID NO. 2 in the list consists of 3...

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Abstract

The invention discloses a plant drought tolerance related MYB transcription factor VcMYB108 as well as a coding gene and application thereof, and relates to the technical field of biology. The VcMYB108 protein provided by the invention is a protein as shown in SEQ ID NO. 2, and the coding gene of the VcMYB108 protein is a DNA (Deoxyribonucleic Acid) molecule as shown in SEQ ID NO. 1. The new gene VcMYB108 is introduced into arabidopsis thaliana to obtain a VcMYB108 transgenic arabidopsis thaliana plant, and experiments prove that the drought tolerance of the VcMYB108 transgenic arabidopsis thaliana plant in the germination period and the seedling period is obviously higher than that of a wild strain and an empty vector transgenic strain, and the VcMYB108 transgenic arabidopsis thaliana plant has no influence on the growth and development of the plant and the yield of fruit pods. The VcMYB108 is a positive regulation transcription factor for the plant to respond to adversity stress, and the drought tolerance of the plant can be improved under the condition that the flowering phase and the yield are not changed. It is suggested that a transgenic line generated after the VcMYB108 gene or the homologous gene thereof is expressed in a receptor plant can improve the drought tolerance of the plant under the condition that the plant growth amount is not sacrificed.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and specifically relates to a transcription factor VcMYB108 related to plant drought tolerance, its coding gene and its application. Background technique [0002] Blueberry (Vaccinium corymbosum L.) is an important perennial economic crop. Its fruit is rich in anthocyanins and has high economic and health value. Blueberries are cultivated in North America, South America, Europe, Asia and many other parts of the world. my country introduced them in the 1980s and realized industrial park production in 2001. Currently, they are in Shandong, Guizhou, Liaoning, Yunnan, Shaanxi and other provinces. It is cultivated in every city, and the market potential is huge. However, blueberry is a shallow-rooted plant with a slender and underdeveloped root system and no root hairs. It is vulnerable to drought stress, resulting in slower growth and lower yields. Screening blueberry's own drought-tolerant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N5/10A01H5/00A01H6/54A01H6/20A01H6/14
CPCC07K14/415C12N15/8218C12N15/8273
Inventor 张凌云王磊曹一博王爱斌
Owner BEIJING FORESTRY UNIVERSITY
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