Classical swine fever E2 protein gene, porcine pseudorabies virus gD protein gene and application thereof

Abstract

The invention relates to a swine fever E2 protein gene, a porcine pseudorabies virus gD protein gene and application thereof. The CHO engineering cell line for stably expressing the swine fever E2 protein and the pseudorabies gD protein is successfully constructed by carrying out gene recombination on the swine fever E2 protein and the pseudorabies gD protein without a transmembrane hydrophobic region and constructing the swine fever E2 protein and the pseudorabies gD protein to pCHO1.0 plasmids and carrying out liposome transfection and dual-pressurization screening on the recombinant plasmids. The hog cholera E2 protein and the pseudorabies gD protein expressed by the CHO engineering cell line constructed by the method are good in immunogenicity and high in expression yield, after immunization, clinical serum and diagnosis and purification of hog cholera and pseudorabies in a pig farm are facilitated, and dual prevention can be achieved through one injection. High economic benefits and market prospects are realized.

Description

Technical field [0001] The present invention is a genetic restructuring vaccine technology field, which involves the Swine fever E2 protein gene, pig pseudo -rabies virus GD protein gene and its application. Background technique [0002] Spine fever (CSF) is a highly contact infectious disease caused by the Classical Swine Fevervirus (CSFV). Its main characteristics are acute types of sewage changes, actual organs bleeding, necrosis and The infarction, chronic types of cellulose necrosis of enteritis. Swine fever is one of the main infectious diseases that endanger the pig farming. In the "National Medium- and Long -term Animal Epidemic Prevention Plan (2012-2020)", swine fever is also listed as one of the major animal epidemic diseases that are preferred and controlled. The CSFV genome leader is about 12.3KB, which contains only a large open reading box (ORF), which is translated into a polymer. By processing, it is processed as a structural protein and 3 cystated glycoprotein, ...

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Problems solved by technology

However, when the attenuated live vaccine is used in combination with E2, there are antigen compatibility problems, and there are still problems that wild strains cannot be distinguished, and the immune effect is easily interfered by maternal antibodies.

[0005] In order to effectively prevent the two diseases of classical swine fever and porcine pseudorabies, and at the same time distinguish the infection of wild strains, there have been many studies on related subunit vaccines at home and abroad, but all of them are single vaccines, and there are no reports on CSFV and PRV dual subunit vaccines.

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