Method for identifying sparus latus microsatellite family and application

Abstract

The invention discloses a sparus latus microsatellite family identification method. The sparus latus microsatellite family identification method comprises the following steps: (1) sparus latus full-sib family establishment; (2) extracting genomic DNA (deoxyribonucleic acid) of parents and offspring of sparus latus; (3) screening polymorphic microsatellite primers; (4) developing and amplifying a fluorescence labeling 8-PCR system; (5) microsatellite site genotyping and family identification: (5.1) determining corresponding genotypes of parents and family individuals on each microsatellite site; and (5.2) performing family identification. According to the method, a paternity test platform is established on sparus latus by utilizing the fluorescence-modified polymorphic microsatellite marker for the first time, and the method is high in identification accuracy, rapid and low in cost. The invention also discloses application of the method in population genetics evaluation, genealogy authentication, paternity test, molecular marker-assisted family management and molecular marker-assisted parent selection of sparus latus.

Description

technical field [0001] The invention belongs to the technical field of fish genetics and breeding, in particular to a method and application of microsatellite family identification of yellowfin sea bream. Background technique [0002] Yellowfin seabream (Acanthopagrus latus), also known as yellow-legged, red-winged, shad, and yellow-finned snapper. It belongs to the genus Perchidae. The body is long and oval, flat and narrow, tall, with a pointed head and a bifurcated tail. The lower lobes of the dorsal, anal, anal, and caudal fins are yellow, and the dorsal fin has 11 spines. Yellowfin sea bream has strong adaptability and grows well in both seawater and brackish water, so it occupies an important position in the aquaculture industry. The distribution of yellowfin sea bream runs through the Indo-West Pacific, from the Persian Gulf along the Indian coast to the Philippines, from Australia to Japan, and my country is mainly distributed in coastal cities such as Guangdong, ...

AI Technical Summary

Problems solved by technology

In the traditional selective breeding of aquatic animals, the breeding unit needs to separate the different families to maintain the family information, which requires a large water body and is inconvenient to manage

In particular, it needs to be considered that there will be some differences in environmental factors between each separate culture pond, and different environmental factors will cause deviations in the estimation of genetic parameters related to breeding

At this time, different families can be mixed together, but it is very complicated to mark all the families

To maintain family information in mixed breeding groups, most animal husbandry research uses physical markers as a means, but physical markers have limitations in aquatic animals such as cumbersome operations, certain effects on growth, and greater damage to larvae

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