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Method for detecting western medicine components contained in Chinese patent medicine

A technology for western medicine ingredients and Chinese patent medicines, applied in the field of Chinese patent medicine detection, can solve the problems of expensive instruments, limited detection sensitivity, long detection time, etc., and achieve the effect of reducing detection costs

Pending Publication Date: 2022-06-07
鹤壁市中医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the conventional detection methods mainly include thin-layer chromatography (TLC), liquid chromatography-mass spectrometry (LC-MS), high-performance liquid chromatography (HPLC), high-performance capillary electrophoresis (HPCE) and near-infrared methods. The first four methods The operation process is relatively cumbersome, and the separation of western medicine components needs to be completed first, the detection time is long, and the equipment is relatively expensive
However, the near-infrared method needs to be realized through a series of algorithms (model building), the detection sensitivity is limited, and it is difficult to realize the simultaneous detection of two or more trace western medicine additives.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] S1, configure the aqueous solution of the Chinese patent medicine to be detected:

[0021] Take 0.6 g of Qingkailing granules added with 0.1% mass ratio of acetaminophen, add 50 mL of water for ultrasonic vibration to fully dissolve, and then add water to dilute to 100 mL to obtain an aqueous solution of the Chinese patent medicine to be detected;

[0022] S2. Take 0.015g of acetaminophen, 1.4g of methacrylic acid, 3.0g of acrylamide, 0.09g of N'N-methylenebisacrylamide, 0.09g of ammonium persulfate, and 4.0mL of deionized water and mix them well in a beaker. After deoxygenation with nitrogen, it was filled into a splint space formed by two glass slides, and then placed in an oven at 60°C for polymerization for 3 hours to obtain a film-like template molecularly imprinted hydrogel;

[0023] S3, extracting the template molecules in the obtained template molecularly imprinted hydrogel to generate an imprinted hydrogel with specific recognition of the western medicine molec...

Embodiment 2

[0029] S1, configure the aqueous solution of the Chinese patent medicine to be detected:

[0030] Take 0.5 g of Xiaoke Pills added with 0.05% glyburide by mass, add 50 mL of water for ultrasonic vibration to fully dissolve it, and then add water to dilute to 100 mL to obtain an aqueous solution of the Chinese patent medicine to be tested;

[0031] S2. Take 0.02 g of glyburide, 1.7 g of methacrylic acid, 4.0 g of acrylamide, 0.14 g of N'N-methylenebisacrylamide, 0.14 g of ammonium persulfate, and 6.0 mL of deionized water and mix them well in a beaker After deoxygenation by nitrogen, it was filled into the splint space formed by two glass slides, and then placed in an oven at 60 °C for polymerization for 3 h to obtain a film-like template molecularly imprinted hydrogel;

[0032] S3, extracting the template molecules in the obtained template molecularly imprinted hydrogel to generate an imprinted hydrogel with specific recognition of the western medicine molecules to be detected...

Embodiment 3

[0038] S1, configure the aqueous solution of the Chinese patent medicine to be detected:

[0039] Take 0.5 g of Gastrodia Uncaria granules with hydrochlorothiazide added at a mass ratio of 0.05%, add 50 mL of water to make it fully dissolved by ultrasonic vibration, and then add water to dilute to 100 mL to obtain an aqueous solution of the Chinese patent medicine to be detected;

[0040] S2. Take 0.03 g of hydrochlorothiazide, 2.0 g of methacrylic acid, 5.0 g of acrylamide, 0.2 g of N'N-methylenebisacrylamide, 0.2 g of ammonium persulfate, and 10.0 mL of deionized water in a beaker and mix them thoroughly. After deoxygenation, it was filled into the splint space formed by two glass slides, and then placed in an oven at 60 °C for polymerization for 3 hours to obtain a film-like template molecularly imprinted hydrogel;

[0041] S3, extracting the template molecules in the obtained template molecularly imprinted hydrogel to generate an imprinted hydrogel with specific recognitio...

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PUM

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Abstract

The invention discloses a method for detecting western medicine components contained in a Chinese patent medicine. The method comprises the following steps: preparing imprinting hydrogel with specific recognition on western medicine molecules to be detected by taking the western medicine components to be detected as template molecules, methacrylic acid and acrylamide as functional monomers, N 'N-methylene bisacrylamide as a cross-linking agent and ammonium persulfate as an initiator; then respectively soaking the imprinted hydrogel in a Chinese patent medicine aqueous solution to be detected and a Chinese patent medicine aqueous solution containing different concentrations of western medicine components to be detected for about 5 minutes, then taking out the imprinted hydrogel, and rinsing the redundant Chinese patent medicine solution with deionized water to obtain a surface enhanced Raman spectrum of the imprinted hydrogel; and comparing the obtained surface enhanced Raman spectrum of the to-be-detected Chinese patent medicine aqueous solution with the surface enhanced Raman spectrum of other samples so as to judge whether the to-be-detected Chinese patent medicine aqueous solution contains the to-be-detected western medicine components or not. Whether the Chinese patent medicine contains the target western medicine component or not can be rapidly detected at low cost, and the content range of the western medicine component is preliminarily judged.

Description

technical field [0001] The invention relates to a method for detecting a Chinese patent medicine, in particular to a method for detecting the western medicine component contained in the Chinese patent medicine. Background technique [0002] With the increasingly fierce competition in the pharmaceutical market, the phenomenon of illegally adding western medicine ingredients into Chinese patent medicines is increasing. At present, conventional detection methods mainly include thin layer chromatography (TLC), liquid chromatography-mass spectrometry (LC-MS), high performance liquid chromatography (HPLC), high performance capillary electrophoresis (HPCE) and near-infrared methods. The first four methods The operation process is cumbersome, and the separation of western medicine components needs to be completed first, the detection time is long, and the equipment is expensive. However, the near-infrared method needs to be implemented through a series of algorithms (building model...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/28G01N1/38
CPCG01N21/658G01N1/28G01N1/38
Inventor 魏新吉
Owner 鹤壁市中医院