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Sweet Arctic shrimp shell polypeptide molybdenum chelate and preparation method thereof

A technology of Arctic sweet shrimp and chelate, applied in the field of materials, can solve problems such as difficult control and harsh microbial fermentation conditions, and achieve the effects of improving production efficiency, reducing production costs, and efficient extraction

Pending Publication Date: 2022-07-01
燕京理工学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the highest recovery rate, but the microbial fermentation conditions are harsh and difficult to control

Method used

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  • Sweet Arctic shrimp shell polypeptide molybdenum chelate and preparation method thereof
  • Sweet Arctic shrimp shell polypeptide molybdenum chelate and preparation method thereof

Examples

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Embodiment 1

[0032] The embodiment of the present invention provides a preparation method of the polar sweet shrimp shrimp shell polypeptide molybdenum chelate.

[0033] The Arctic sweet shrimp and shrimp shells are the solid wastes of shrimp heads and shrimp shells produced by aquatic companies and restaurants.

[0034] The collected shrimp shell raw materials are frozen and stored for later use, dried in an oven at 60-80° C. when in use, and pulverized by a pulverizer after drying to obtain shrimp shell powder for later use.

[0035] The compound enzyme was obtained by compounding 800u / mg papain, 250u / mg trypsin and 50u / mg trypsin in a mass ratio of 71.2:22.4:10.5.

[0036] Weigh the shrimp shell powder and the composite enzyme in a mass ratio of 10000:1, add them into deionized water, and measure the deionized water according to 10 mL of deionized water per gram of shrimp shell powder, and further add 0.1 mol / g of the total mass of the composite enzyme to 0.1% L of sodium chloride as a...

Embodiment 2

[0042] The embodiment of the present invention provides a preparation method of the polar sweet shrimp shrimp shell polypeptide molybdenum chelate.

[0043] The process of this example is basically the same as that of Example 1, except that the addition amount of 0.1 mol / L sodium chloride solution is 0.2% of the total mass of the composite enzyme.

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Abstract

The invention relates to a preparation method of an Arctic sweet shrimp shell polypeptide molybdenum chelate, which sequentially comprises the following steps of: (1) drying and crushing shrimp shell raw materials to obtain shrimp shell powder; (2) preparing a compound enzyme solution; (3) mixing the compound enzyme liquid, shrimp shell powder and deionized water, and performing enzymolysis to obtain hydrolyzed polypeptide liquid; (4) dissolving molybdenum trioxide with a sodium hydroxide solution to prepare a sodium molybdate solution; and (5) mixing the sodium molybdate solution with the hydrolyzed polypeptide solution, carrying out chelation reaction, precipitating with ethanol after the chelation reaction is finished, filtering, washing and drying to obtain the product. The compound enzyme liquid comprises papain, trypsin, neutral protease and an enzyme activator. The invention further discloses the pandalus arctii shell polypeptide molybdenum chelate prepared by the method. By compounding three different enzymes and adding the enzyme activator, the extraction rate and the chelation rate of the polypeptide in the shrimp shell are remarkably improved, and efficient preparation of the Arctic sweet shrimp shell polypeptide molybdenum chelate is achieved.

Description

technical field [0001] The invention relates to the field of materials, in particular to an arctic sweet shrimp shell polypeptide molybdenum chelate compound and a preparation method thereof. Background technique: [0002] At present, the methods of extracting polypeptides from shrimp shells include lye extraction method, alkali solution acid precipitation extraction method, salt solution extraction method, ultrasonic-assisted enzyme extraction method, microbial fermentation method, etc. [0003] The lye extraction method uses sodium hydroxide solution for extraction, or is assisted by ultrasonic waves. The method is simple in operation and low in cost, but causes great environmental pollution, the degree of protein hydrolysis during extraction is difficult to control, and the extracted protein structure is also damaged to varying degrees. [0004] The alkali-soluble acid precipitation extraction method is to dissolve the protein in an alkaline solution, and then adjust the...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/02C07K1/107C07K1/14
CPCC12P21/06C07K1/02C07K1/1077C07K1/145
Inventor 刘雪凌陈旭华刘湘
Owner 燕京理工学院
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