Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of silk fibroin repair patch and silk fibroin repair patch

A silk fibroin and patch technology, applied in the field of tissue repair materials, can solve problems such as the difficulty in making silk fibroin repair patches

Pending Publication Date: 2022-07-05
NAT INST OF ADVANCED MEDICAL DEVICES SHENZHEN
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the traditional preparation method of silk fibroin repair patch is difficult to prepare a silk fibroin repair patch with uniform fiber distribution.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of silk fibroin repair patch and silk fibroin repair patch
  • Preparation method of silk fibroin repair patch and silk fibroin repair patch
  • Preparation method of silk fibroin repair patch and silk fibroin repair patch

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] like figure 1 The preparation method of the silk fibroin repair patch of one embodiment shown, comprises the following steps:

[0037] S10, providing a formic acid solution of silk fibroin, and the mass concentration of the formic acid solution of silk fibroin is 6%-15%.

[0038] In this embodiment, formic acid is used as a solvent for silk fibroin, and formic acid is a volatile substance, so as to ensure that the finally prepared silk fibroin repair patch does not contain toxic substances.

[0039] In the present invention, the control of the mass concentration of the formic acid solution of silk fibroin is extremely important. If the concentration is too low, microspheres and beads are likely to appear, which will affect the performance of the silk fibroin repair patch. Electrospinning.

[0040] More preferably, the mass concentration of the formic acid solution of silk fibroin is 10%.

[0041] Preferably, in this embodiment, the formic acid solution of silk fibroi...

Embodiment 1

[0073] Weigh the silkworm cocoon (Sichuan Silkworm No. 26), cut it into pieces, wash it three times with ultrapure water to remove impurities, and prepare 0.02M Na 2 CO 3 solution and heat to boil, add silkworm cocoon fragments to the boiling Na 2 CO 3 solution, boil for 30min, repeat 3 times. Next, the silkworm cocoons were soaked in ultrapure water at 60°C, stirred and washed, washed for 30 minutes, and repeated 3 times. Finally, the silkworm cocoons were placed in an oven at 40°C overnight, and dried to obtain silk fibroin.

[0074] According to the solid-liquid ratio of 1:4, the silk fibroin was dissolved in a LiBr solution with a concentration of 9.3 mol / L to obtain a mixed solution. The mixed solution was transferred to a dialysis bag with a molecular weight cut-off of 8000, and then the dialysis bag was placed in Dialysis was performed in ultrapure water. After the dialysis was completed, the liquid in the dialysis bag was centrifuged, and the supernatant was retaine...

Embodiment 2

[0078] Weigh the silkworm cocoon (Sichuan Silkworm No. 26), cut it into pieces, wash it three times with ultrapure water to remove impurities, and prepare 0.02M Na 2 CO 3 solution and heat to boil, add silkworm cocoon fragments to the boiling Na 2 CO 3 solution, boil for 30min, repeat 3 times. Next, the silkworm cocoons were soaked in ultrapure water at 60°C, stirred and washed, washed for 30 minutes, and repeated 3 times. Finally, the silkworm cocoons were placed in an oven at 40°C overnight, and dried to obtain silk fibroin.

[0079] According to the solid-liquid ratio of 1:4, the silk fibroin was dissolved in a LiBr solution with a concentration of 9.3 mol / L to obtain a mixed solution. The mixed solution was transferred to a dialysis bag with a molecular weight cut-off of 8000, and then the dialysis bag was placed in Dialysis was performed in ultrapure water. After the dialysis was completed, the liquid in the dialysis bag was centrifuged, and the supernatant was retaine...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of a silk fibroin repair patch and the silk fibroin repair patch prepared by the preparation method. The preparation method of the silk fibroin repair patch comprises the following steps: providing a formic acid solution of silk fibroin; performing electrostatic spinning by taking the formic acid solution of the silk fibroin as a raw material to obtain a silk fibroin fiber membrane; and soaking the silk fibroin fiber membrane in a methanol solution, fully soaking until the silk fibroin fiber membrane is subjected to crosslinking denaturation, taking out and drying to obtain the silk fibroin repair patch. In combination with data of a test case part in the specification, fibers in the silk fibroin repair patch prepared by the preparation method of the silk fibroin repair patch are uniform in diameter and distribution, pores exist among the fibers, adhesion growth of cells can be met, and the cells can grow into the silk fibroin repair patch.

Description

technical field [0001] The invention relates to the technical field of tissue repair materials, in particular to a preparation method of a silk fibroin repair patch and a silk fibroin repair patch prepared by the preparation method of the silk fibroin repair patch. Background technique [0002] The regeneration and repair of human tissue damage has received more and more attention. With the advancement of medical technology and the needs of patients for a high-quality life, tissue repair patches are widely used in surgical operations as a new type of medical implant device. The tissue repair patch can not only play the role of tissue mechanical support, but also has good biocompatibility and pore structure, which can meet the adhesion, growth and proliferation of cells, repair defective tissue, and speed up postoperative recovery. The restorative clinical field is widely recognized and accepted. [0003] At present, the common tissue repair patches in clinic mainly include ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/50A61L27/58D04H1/728
CPCA61L27/227A61L27/50A61L27/58D04H1/728A61L2300/412C08L89/00
Inventor 宋晓杰王佳冕张红斌贾伟胡楠
Owner NAT INST OF ADVANCED MEDICAL DEVICES SHENZHEN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com