Composite functional bacterial agent for in-situ remediation of contaminated soil by taking pseudomonas stutzeri as carrier and application of composite functional bacterial agent

Abstract

The invention discloses a composite functional bacterial agent for in-situ remediation of contaminated soil by using pseudomonas stutzeri as a carrier and application of the composite functional bacterial agent, the composite functional bacterial agent comprises pseudomonas stutzeri VTCQ-1 and pseudomonas aeruginosa VTS-1, the preservation number of the pseudomonas stutzeri VTCQ-1 is CGMCC24469, the preservation number of the pseudomonas aeruginosa VTS-1 is CGMCC2200, the preservation number of the pseudomonas stutzeri VTCQ-1 is CGMCC24469, and the preservation number of the pseudomonas aeruginosa VTS-1 is CGMCC2200. Wherein the pseudomonas stutzeri VTCQ-1 is used as a carrier of the pseudomonas aeruginosa VTS-1. According to the characteristics that the specific surface area of a bacterial colony of the pseudomonas stutzeri VTCQ-1 is large and a large and stable attachment place can be provided for other bacteria, the pseudomonas stutzeri VTCQ-1 and the pseudomonas aeruginosa VTS-1 are subjected to mixed culture, the survival rate of the pseudomonas aeruginosa VTS-1 can be increased, the prepared composite functional bacterial agent can improve the degradation efficiency of hydrocarbons in soil, and the degradation efficiency of the hydrocarbons in the soil is improved. The hydrocarbon polluted soil treatment period is greatly shortened, and the diversity and durability of soil microorganisms are maintained.

Description

technical field

[0001] The present invention relates to the field of bioremediation of petroleum hydrocarbon-contaminated soil, in particular to a composite functional bacterial agent for in-situ remediation of contaminated soil using Pseudomonas stutzeri as a carrier and its application. Background technique

[0002] At present, there are many methods in the field of in-situ remediation of hydrocarbon-contaminated soil, such as physical, chemical, and microbial methods. Among them, microbial in-situ remediation of hydrocarbon-contaminated soil is the most environmentally friendly, economical, free of secondary pollution, and more reliable in terms of sustainability and environmental friendliness. This method has attracted extensive attention and in-depth research in various environmental protection fields around the world.

[0003] The key to microbial remediation methods is the selection of efficient hydrocarbon-degrading bacteria and the maintenance of bacterial flora in ...

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