Agrobacterium tumefaciens for promoting postharvest potato tuber callus

Abstract

The invention provides an Agrobacterium tumefaciens strain for promoting callus of harvested potato tubers, which is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Agrobacterium tumefaciens strain is CGMCC No: 24362. The Agrobacterium tumefaciens strain provided by the invention is separated from a self-callus potato tissue, and has the advantages that the propagation is fast, the strain is easy to collect, the post-harvest tuber is easy to treat, the generation speed of a tuber callus epidermis sealing layer (namely secondary peridermis which is mainly composed of a cell layer of lignin, polyphenol cork grease and polyester cork grease) is accelerated, and the survival rate of the tuber callus epidermis sealing layer is increased. Therefore, the weight loss speed of tubers and the expansion of fusarium infected disease spots are reduced.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, in particular to an Agrobacterium tumefaciens which promotes the callus of postharvest potato tubers. Background technique [0002] Potato (Solanum tuberosum L.) is an important food and vegetable crop. The tuber is rich in starch, vitamins and minerals, and has high nutritional value. It feeds about 1 billion people on the earth. Potatoes are cultivated in more than 150 countries and regions around the world. my country is a big potato producing country. With the continuous expansion of potato planting area, potato occupies an increasingly important position in my country's agricultural production. However, the epidermis of potato tubers is crisp and tender, and the harvesting and post-harvest storage and transportation processes are extremely susceptible to mechanical damage. These wounds make the tuber tissue easy to lose water, and become the main channel for pathogens to i...

AI Technical Summary

Problems solved by technology

However, the skin of potato tubers is crisp and tender, and they are extremely vulnerable to mechanical damage during harvesting and post-harvest storage. These wounds make the tuber tissue easy to lose water, and become the main channel for pathogenic bacteria to invade the tubers, resulting in a decline in the quality of the tubers during storage and serious rot.

[0003] Although the potato tuber epidermis can heal naturally after mechanical damage, it takes a long time. During the self-healing process of the wound for a long time, the probability of pathogen infection of the tuber increases, which increases the postharvest rot rate.

At the same time, in commercial production, potatoes need to be stored in the warehouse as soon as possible after harvesting, so as to reduce the places required for turnover and the labor of handling, thus resulting in the lack of conditions for self-healing after harvesting, and poor healing effect, which increases the risk of rotten cellars during storage. risk

The defect of slow self-healing speed of tuber epidermis greatly affects the management of harvesting, postharvest storage and transportation of potato tubers

Although there have been some reports that the use of high temperature or chemical agents can promote the formation of secondary periderm in the wounded part of potato tubers, however, excessively high temperature will easily increase the physiological metabolism of postharvest tubers and accelerate the quality deterioration, chemical drug treatment will be accompanied by drug residue problem

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