Unlock instant, AI-driven research and patent intelligence for your innovation.

SNP (Single Nucleotide Polymorphism) molecular marker related detection primer or probe for major QTL (Quantitative Trait Loci) of oil content character of cabbage type rape seeds and application of SNP molecular marker related detection primer or probe

A Brassica napus and molecular marker technology, which is applied to the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., to achieve the effects of ingenious design, suitable for large-scale popularization and application, and low cost

Pending Publication Date: 2022-07-12
贵州省油菜研究所
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It is difficult to make major breakthroughs by relying on traditional breeding techniques. Therefore, combining molecular marker technology and quantitative genetic methods provides new molecular markers for the genetic breeding of rapeseed oil content.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP (Single Nucleotide Polymorphism) molecular marker related detection primer or probe for major QTL (Quantitative Trait Loci) of oil content character of cabbage type rape seeds and application of SNP molecular marker related detection primer or probe
  • SNP (Single Nucleotide Polymorphism) molecular marker related detection primer or probe for major QTL (Quantitative Trait Loci) of oil content character of cabbage type rape seeds and application of SNP molecular marker related detection primer or probe
  • SNP (Single Nucleotide Polymorphism) molecular marker related detection primer or probe for major QTL (Quantitative Trait Loci) of oil content character of cabbage type rape seeds and application of SNP molecular marker related detection primer or probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1. Determination of the phenotype of the seed oil content trait of the Brassica napus parent and RIL population

[0048] (1) Using Brassica napus Darmor and white mustard to carry out distant hybridization, selecting the large-grain low-oil-content line GRG2462 in F2:3 as the female parent, and selecting the small-grain high-oil-content line GRD328 and GRG2462 for hybridization, The recombinant inbred lines composed of 158 lines obtained by selfing for 11 consecutive generations (Darmor of Brassica napus are from Wuhan Oil Crop Germplasm Resource Bank, and white mustard and small-grain lines GRD328 were purchased from Guizhou Hemufu Seeds Co., Ltd. Company), in Guizhou Province for many years (2012-2013 Guiyang, 2013-2014 Qinghe, 2014-2015 Changshun, 2015-2016 Guiyang, 2015-2016 Jinsha Changshun, 2016-2017 Changshun, 2017-2018 Tangtou, 2017-2018 Jinsha, 2018-2019 Changshun) seed oil content traits were investigated.

[0049] (2) Using live seedlings, row spacin...

Embodiment 2

[0052] Example 2, the acquisition of high-quality SNP data set of RIL population

[0053] The CTAB method was used to extract the total leaf DNA, and the total leaf DNA of each material of the RIL population was extracted. The specific method is as follows:

[0054] Rinse the young leaves in 10% ethanol; then cut 0.1-0.2 g of leaves into a grinding bowl, quickly grind with liquid nitrogen to powder, and put them into a 2 mL centrifuge tube; add 700 μL of preheated DNA extraction solution; After mixing, put it in a 65°C water bath for 1 hour, and mix once every 10-15 minutes; add 700 μL of mixed solution (phenol:chloroform:isoamyl alcohol=25:24:1), gently invert and mix for 10 minutes; at room temperature, Centrifuge at 10 000 × g for 15 min; pipette the supernatant into a new 2 mL centrifuge tube; add an equal volume of mixed solution (chloroform: isoamyl alcohol = 24: 1), invert and mix, let stand for 5 min, 10000 × g, centrifuge for 15 min , suck the supernatant into a new ...

Embodiment 3

[0056] Embodiment 3, the construction of high density genetic map

[0057] According to the above Example 2, a high-quality population SNP dataset was obtained and constructed using MadMapper (http: / / cgpdb.ucdavis.edu / XLinkage / MadMapper / ) and HighMap software (http: / / highmap.biomarker.com.cn / ). High-density SNP genetic linkage map of the RIL population. In order to improve the speed of operation, firstly, use the RECBIT program in MadMapper software to remove redundant SNPs (two or more SNPs show co-segregation in the population), data missing ratio ≥ 25% of SNPs in the 158 lines, and SNPs in the population. SNPs with an allelic segregation ratio figure 2 shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker of a major QTL (Quantitative Trait Loci) of an oil content character of a brassica napus seed, the SNP molecular marker is closely linked with the major QTL of the oil content character of the brassica napus seed, and the major QTL of the oil content character of the brassica napus seed is located between the 11378925 base and the 11559658 base of an A07 chromosome of the brassica napus. The invention further provides application of the SNP molecular marker, a primer or a probe for detecting the SNP molecular marker and application of the primer or the probe. The SNP molecular marker of the major QTL locus of the oil content character of the brassica napus seeds can detect the oil content of the brassica napus seeds, can predict the oil content of the brassica napus seeds, and can effectively select the oil content of the brassica napus seeds; the molecular marker can also be used for molecular marker-assisted breeding of brassica napus with high seed oil content, accelerates the process of seed oil content breeding of brassica napus, and is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical field of molecular biology and rape genetics and breeding, in particular to the technical field of oil content traits of Brassica napus seeds, in particular to a SNP molecular marker and related detection of the main QTL site of the oil content trait of Brassica napus seeds Primers or probes, and applications. Background technique [0002] Rapeseed is the largest oil crop in my country, providing 5.2 million tons of high-quality edible oil every year, accounting for more than 47% of domestic vegetable oil. In recent years, my country's foreign dependence on edible oil is as high as 65%, and the import of oil plants is greatly affected by the uncertainty of international trade. Oil content of rapeseed seeds is mainly controlled by maternal effect, embryonic gene effect, pollen instinct, cytoplasmic effect and corresponding gene-environment interaction effect, which is in line with additive-dominant-epistatic genet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 向阳赵传纪韩宏仕侯燕赵继献
Owner 贵州省油菜研究所