Marker and application thereof in preparation of product for evaluating immune function of organism

Abstract

The invention relates to the technical field of biology, in particular to a marker and application thereof in preparation of a product for evaluating the immune function of an organism. The invention discloses a molecular mechanism that rapamycin mechanical target protein (mTOR) regulates and controls functions of different subtypes of CD3 + CD4 + T cells for the first time, and verifies that mTOR pathway key effector molecule phosphorylated ribosomal protein S6 combined with fork head box protein P3 (Forkhead box P3, FOXP3) is used as markers, and the molecular mechanism of the rapamycin mechanical target protein (mTOR) for regulating and controlling functions of different subtypes of CD3 + CD4 + T cells is established through a multi-dimensional analysis technology. The method can accurately judge the composition proportion and the biological function change condition of different subtype CD3 + CD4 + T cells on the periphery of a normal person and a patient, has the advantages of high sensitivity and high specificity, and can evaluate the influence of diseases and drugs on immune cells from the pathophysiology mechanism, so that the defects of the existing clinical examination technology at present are overcome, and the clinical examination efficiency is improved. The method has important technical innovation and clinical application value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a marker and its application in preparing a product for evaluating the immune function of the body. Background technique [0002] Infection has always been the most serious threat to human health. The immune function of the body is the most important line of defense against the invasion of various foreign pathogens. In the disease state, patients may be immunocompromised. At the same time, various immune intervention treatment programs significantly affect the body's immunity, resulting in a significantly higher risk of opportunistic pathogen infection. Timely and accurate assessment of a patient's immune level is crucial to guiding clinicians to formulate or adjust treatment plans, especially for patients who require long-term immunosuppressive treatment, which is a life-threatening issue. For a long time, clinicians are accustomed to use blood routine tests, immunoglobulin (IgG)...

AI Technical Summary

Problems solved by technology

[0004] Multiparameter flow cytometry is a new technology that has been continuously developed in the field of biomedical basic research and has been initially verified in clinical work. However, even for flow cytometry detection of CD3+CD4+T cell typing, which is the most widely used clinical There are still some deficiencies that need to be improved

First of all, the current CD3+CD4+T cell flow cytometry detection indicators commonly used in clinical practice are limited to cell surface markers (CD3, CD4, CD8, CD25, etc.), which can only reflect changes in the number or proportion of T cells in different subgroups , cannot directly reflect the changes in the biological functions of CD3+CD4+T cells, and cannot help clinicians make timely judgments on the immunity of patients; secondly, CD3+CD4+T cells express a wide variety of cytokines (interleukin family, interference family of genes, etc.), and the biological functions of a certain factor in different cells are overlapping and multidirectional, and it is often necessary to simultaneously measure the levels of multiple surface markers and cytokines of CD4+ T cells in clinical practice, and perform complex combination Reliable analysis results can only be obtained through reliable analysis, which will lead to the consumption of a large amount of antibody reagents and blood samples in the entire evaluation process, and the manpower and material resources required for the result analysis are high and time-consuming; again, using flow cytometry to detect CD4+ at the same time A variety of (>4) target proteins of T cells inevitably have fluorescence interference between different channels and the impact of non-specific binding between various antibodies. The more indicators tested, the sensitivity and specificity of flow cytometry will be interfered. The larger the size, the more limited its clinical promotion; finally, the current clinical flow cytometry detection methods are seriously behind the basic research field, and there is a lack of simple and reliable new biomarkers and biomarkers for specific evaluation of CD3+CD4+ T cell pathophysiological functions. Testing method

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