Breeding method of novel nuclear male sterile line of rice

Abstract

The invention relates to a breeding method of a novel nuclear male sterile line of rice. The sterility of the novel nuclear male sterile line of the rice is mainly controlled by a pair of alleles S + and S-on a 10th chromosome, and the novel nuclear male sterile line (S + S-, S + / -for short) has the characteristic of low selfing fruiting under low-temperature and short-day conditions, and selfing is performed until the genetic background is homozygous and stable. Sterile strains (S + / -) which do not meet requirements are eliminated through test cross, and only a small number of target single plants are subjected to near-isogenic line breeding work. S + S + and S-S-near-isogenic lines can be separated from the selfing offspring of the novel nuclear male sterility target single plant and can be directly used for seed propagation of the novel nuclear male sterility line, and the breeding efficiency is greatly improved. A conventional bridge parental method only can utilize stable materials without S + / -genes, the procedure is complex, and the workload is large. According to the method, almost all germplasm resources can be utilized according to breeding needs, operation is easy, the utilization range of the germplasm resources is expanded, and the breeding efficiency of near-isogenic lines is greatly improved.

Description

technical field [0001] The invention relates to the technical field of rice hybrid breeding, in particular to a breeding method for a new type of nuclear male sterile line of rice. Background technique [0002] As we all know, the utilization of heterosis is an effective way to increase crop yield. The three-line hybrid rice is limited by the relationship between restoration and protection, the combination is not free, and the seed production process is complicated, which restricts the further development of rice heterosis utilization. Shi Mingsong found male sterile plants in the japonica rice Nongken 58. Subsequent studies have shown that the found sterile plant is sterile in long days at high temperature and fertile at low temperature in short days (mainly temperature-sensitive, hereinafter referred to as high-temperature-sensitive male sterility), referred to as "Nongken 58S". Then Shi Mingsong put forward the idea of ​​"one line with two uses" for the utilization of ri...

AI Technical Summary

Problems solved by technology

The general fertile bridge parents are difficult to test the combining ability. Wait until the near-isogenic line is bred to obtain a new type of nuclear male sterile line before testing the combining ability. At least 90% of the lines need to be eliminated, which will waste a lot of manpower and material resources

Moreover, the workload of obtaining near-isogenic lines through bridge parents is twice the workload of backcrossing three lines into sterile lines from the maintainer line, which is extremely difficult.

Some inventions refer to the direct use of the existing three-line backbone maintainer line or two-line sterile line as the bridge parent. The number is extremely limited and it is difficult to improve. The significance is not too great

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