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Aggregation-induced emission microsphere based on N-hydroxyethyl-1, 8-naphthalimide tetraphenyl ethylene derivative and application of aggregation-induced emission microsphere

A technology of naphthalimide tetraphenylethylene and aggregation-induced luminescence, which is applied in the field of medical analysis and detection, can solve problems such as low sensitivity, weak probe signal strength, and cannot meet the needs of high-sensitivity quantitative detection, and achieve high sensitivity High, easy-to-operate effect

Pending Publication Date: 2022-07-22
NANCHANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional immunochromatographic method has low sensitivity due to weak probe signal strength, and cannot meet the needs of some highly sensitive quantitative detection

Method used

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  • Aggregation-induced emission microsphere based on N-hydroxyethyl-1, 8-naphthalimide tetraphenyl ethylene derivative and application of aggregation-induced emission microsphere
  • Aggregation-induced emission microsphere based on N-hydroxyethyl-1, 8-naphthalimide tetraphenyl ethylene derivative and application of aggregation-induced emission microsphere
  • Aggregation-induced emission microsphere based on N-hydroxyethyl-1, 8-naphthalimide tetraphenyl ethylene derivative and application of aggregation-induced emission microsphere

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of aggregation-induced luminescent microspheres

[0040]N-2-(4-(Tristyryl-)-phenoxy-)-ethyl)-4-(4-(tristyryl)-phenyl)-1,8-naphthalene dicarboyl The imine is dissolved in tetrahydrofuran solution as AIE molecule, which is liquid A. The polystyrene microspheres on the surface of carboxyl groups are dispersed in the aqueous solution of sodium dodecyl sulfonate, which is liquid B. First, liquid A is added to acetone and mixed well. Add liquid B while stirring again, at room temperature magnetic stirring for 10-20h to evaporate acetone to a minimum amount, centrifuging to obtain the product and washing with ultrapure water, the obtained fluorescent polystyrene nano-microspheres (i.e. based on N-2-(4-(tri) Aggregation-induced luminescent microspheres) suspension of styryl-)-phenoxy-)-ethyl)-4-(4-(tristyryl)-phenyl)-1,8-naphthalimide Disperse in ultrapure water for later use, the SEM of the microspheres is as follows image 3 shown.

Embodiment 2

[0041] Example 2 Preparation and application of immunochromatographic test strips

[0042] 1. Preparation of Nitrocellulose Membrane

[0043] The NT-proBNP-coated antibody was coated on a nitrocellulose membrane: the concentration of the NT-proBNP-coated antibody was diluted with PB 6.5 to 1.8 mg / mL, and the resulting solution was sprayed on the membrane as a detection line; the concentration of the diluted secondary antibody was 1 mg / mL, the obtained solution was sprayed on the film as a control line, the spray volume of both lines was 0.74 μL / cm, the distance between the detection line and the top edge of the film was 10 mm, the interval between the two lines was 5 mm, dried at 37 °C for 12 hours, and placed Store in a dry cabinet for later use.

[0044] 2. Preparation of Binding Pads

[0045] Add 10 μg AIE fluorescent microspheres to 500 μL PB 6.5 buffer, then add 2 μg labeled antibody, add 4 μg EDC to activate the carboxyl groups on the surface of the microspheres after ...

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Abstract

The invention discloses an aggregation-induced emission microsphere based on an N-hydroxyethyl-1, 8-naphthalimide tetraphenyl ethylene derivative and application of the aggregation-induced emission microsphere, and belongs to the field of analysis and detection. The aggregation-induced emission microspheres are prepared by taking N-hydroxyethyl-1, 8-naphthalimide tetraphenylethylene derivatives as AIE molecules through a swelling method, then the aggregation-induced emission microspheres are used for preparing the immunochromatography test strip for quantitatively detecting amino-terminal B-brain natriuretic peptide, and the test strip structurally comprises a PVC (polyvinyl chloride) bottom plate, a nitrocellulose membrane, a binding pad, a sample pad and a water absorption pad. An aggregation-induced emission microsphere-labeled anti-amino-terminal B brain natriuretic peptide labeled antibody is sprayed on a combination pad of the immunochromatography test strip, the nitrocellulose membrane is provided with a detection area (T line) and a control area (C line), and a specific labeled antibody (coated antibody) and an anti-immune globulin G antibody (secondary antibody) are respectively sprayed on the detection area (T line) and the control area (C line). Compared with a traditional FITC fluorescent test strip, the sensitivity is improved by 14 times, and the fluorescent test strip has the advantages of being easy and convenient to operate, rapid in quantification, convenient to carry, small in sample dosage and the like.

Description

technical field [0001] The invention belongs to the technical field of medical analysis and detection, and in particular relates to aggregation-induced luminescent microspheres based on N-hydroxyethyl-1,8-naphthalimide tetrastyrene derivatives and a preparation method and application thereof. Background technique [0002] Brain natriuretic peptide (BNP) is a hormone distributed in the heart, which mainly plays a role in myocardial tissue. In addition, measurements of BNP and Nterminal pro-brainnatriuretic peptide (NT-proBNP) can also serve as diagnostic biomarkers for acute and chronic heart failure. Since NT-proBNP is not easily degraded and has a long half-life, it can be used as an ideal predictor. Therefore, the development of a fast, simple, and inexpensive rapid detection method to quantitatively detect NT-proBNP in real time is of great significance for clinical diagnosis and later treatment determination. [0003] Immunochromatography is a detection method based on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06B01J13/02G01N21/64G01N33/548G01N33/558G01N33/58G01N33/74
CPCC09K11/06C09K11/025B01J13/02G01N21/6428G01N33/74G01N33/558G01N33/548G01N33/582G01N33/587G01N2021/6439C09K2211/1029C09K2211/1007G01N2333/58
Inventor 李响敏程泉凯仲海澄黄小林熊勇华郭亮孔蕴源刘洋范潇璟
Owner NANCHANG UNIV
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