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< 131 > I-HSA-ICG nanoparticles as well as preparation method and application thereof

A technology of 131I-HSA-ICG and nanoparticles, applied in the field of 131I-HSA-ICG nanoparticles and its preparation, to achieve the effects of high water solubility, high fluorescence efficiency and stability, and high affinity

Active Publication Date: 2022-08-05
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previous studies have found that in the field of breast cancer, prostate cancer, colorectal cancer, liver cancer and other cancer treatment research, there are studies on the use of HSA-ICG nanoparticles combined with other chemotherapy drugs to treat tumors, but 131 The study of I-HSA-ICG nanoparticles for the integration of diagnosis and treatment of human undifferentiated thyroid cancer has not been reported

Method used

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  • &lt; 131 &gt; I-HSA-ICG nanoparticles as well as preparation method and application thereof
  • &lt; 131 &gt; I-HSA-ICG nanoparticles as well as preparation method and application thereof
  • &lt; 131 &gt; I-HSA-ICG nanoparticles as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example 1

[0040] Preparation of HSA-ICG Nanoparticles

[0041] According to the molar ratio of HSA:ICG=1:1, weigh 20 mg of HSA and dissolve it in 4 mL of H 2 O, corresponding to ICG 0.234mg dissolved in 1mL H 2 O, the reaction system is 5mL, 0.117mL of 2mg / mL ICG solution can be mixed with water to 1mL, stirred and reacted with 4mL HSA solution for 1h, ultrafiltration and centrifugation (molecular weight cut-off 30kDa, rotating speed 6000r / min, 10min, rinse 3 times), the ultrafiltration The solution was expanded to 5 mL, and placed in a petri dish for 30 min in a refrigerator at 4°C, 2 hours in a -20°C refrigerator, and overnight in a -80°C refrigerator. The purified HSA-ICG nanoparticles were freeze-dried by a freeze dryer, and stored in a refrigerator at 4°C away from light for subsequent experiments.

Embodiment 1

[0043] Preparation of I-HSA-ICG nanoparticles

[0044] (1) Prepare 0.01M PB buffer: weigh 7.16g Na 2 HPO 4 -12H 2 O and 3.12g NaH 2 PO 4 -2H 2 O was dissolved in 100ml aqueous solution, and 19mL 0.2mol / mL Na 2 HPO 4 -12H 2 O solution, 81 mL of 0.2 mol / mL Na 2 HPO 4 -12H 2 O solution, mix evenly (0.2M, pH=7.4), take 50mL, add water and dilute to 1000mL for later use.

[0045] (2) Weigh 20 mg of HSA, 5 mg of chloramine T, and 5 mg of sodium metabisulfite, respectively, and dissolve them in 1 mL of PB buffer, NaI 2H 2 O 930 mg dissolved in 1 mL of water. HSA, chloramine T, NaI 2H 2 The O solution was mixed, and after the reaction was shaken by a shaker for 1 min, sodium bisulfite solution (5 mg / mL, 1 mL) was added to terminate the reaction (reaction for 1 min). The solution after the above reaction was ultrafiltered (molecular weight cut-off 30kDa, rotational speed 6000r / min, 10min, rinsed 3 times), and then high-purity water was added to expand the volume to 4mL. ...

Embodiment 2

[0047] 131 Preparation of I-HSA-ICG nanoparticles

[0048] According to the needs of the experiment, put on a lead coat, and extract a certain unit count of radioactive Na in the fume hood 131 I solution (volume is less than 1mL, the count is greater than the target count by at least 1 unit), label HSA according to the chloramine T method and ultrafiltration centrifuge (molecular weight cut-off 30kDa, rotating speed 6000r / min, 10min, rinse 3 times), re-measure the filtrate radioactivity counts (labeling rate above 90%), and then the radioactivity 131 The I-HSA solution is reacted with the prepared ICG solution (the steps are the same as above), and the reacted solution is ultrafiltered and centrifuged to 250 μL for use.

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Abstract

The invention discloses a < 131 > I-HSA-ICG nano-particle as well as a preparation method and application thereof, and belongs to the technical field of nano-drug preparation. HSA is used as a carrier, radioactive < 131 > I is labeled in a neutral environment through a chloramine T method, and ICG is non-covalently bound to < 131 > I-HSA nanoparticles through a stirring reaction, so that the < 131 > I-HSA-ICG nanoparticles are prepared. The < 131 > I-HSA-ICG nanoparticles prepared by the invention not only can be used for local tumor bimodal imaging, but also can be used for simultaneously performing photothermal therapy and radionuclide therapy on human undifferentiated thyroid cancer. The synthesis method of the < 131 > I-HSA-ICG nano-particles is simple, easy to repeat, low in toxicity and effective and remarkable in treatment effect, and a new thought is provided for treatment research of the undifferentiated thyroid cancer.

Description

technical field [0001] The invention relates to the technical field of nanomedicine preparation, in particular to a 131 I-HSA-ICG nanoparticles and their preparation methods and applications. Background technique [0002] In recent years, the prevalence of thyroid cancer has been increasing year by year in the world, and it has become one of the major diseases affecting people's health. For differentiated thyroid cancer (DTC), surgery combined with radioactive iodine therapy followed by levothyroxine replacement therapy is the accepted procedure. Iodine is an important component of thyroid hormone synthesis by follicular cells in normal thyroid tissue. The sodium iodide transporter (NIS) in DTC is expressed on the cell basement membrane, providing a physiological basis for the active transport of iodide ions to thyroid follicular cells. DTC cells maintain similar functions to follicular cells, such as iodine uptake and iodination, while undifferentiated thyroid cancer cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/08A61K51/12A61K49/00A61K101/02
CPCA61K51/081A61K51/1244A61K49/0034A61K49/0056A61K49/0093Y02A50/30
Inventor 孟召伟张雪梅李宁谭建贾强申一鸣闫紫玉张春梅
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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