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Double-layer artificial blood vessel and preparation method thereof

A kind of artificial blood vessel, double-layer technology, applied in the field of double-layer artificial blood vessel and its preparation, can solve the problems of blood vessel anti-compression ability and anti-exudation ability weakening, high permeability, etc., to achieve the formation of endothelial cell layer, hydrophobicity Prevention, high porosity effect

Pending Publication Date: 2022-08-05
ZHONGNAN HOSPITAL OF WUHAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, larger porosity is often accompanied by higher permeability, resulting in weakened anti-compression and anti-exudation capabilities of tissue engineering blood vessels.

Method used

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  • Double-layer artificial blood vessel and preparation method thereof
  • Double-layer artificial blood vessel and preparation method thereof
  • Double-layer artificial blood vessel and preparation method thereof

Examples

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preparation example Construction

[0039] According to a typical embodiment of the present invention, a method for preparing a double-layer artificial blood vessel is provided, such as figure 1 As shown, the method includes:

[0040] Step S1, dissolving polycarbonate polyurethane and silk fibroin together in HFIP (hexafluoroisopropanol) to obtain a mixed solution of polycarbonate polyurethane and silk fibroin;

[0041] In the mixed solution, the concentration of the polycarbonate polyurethane is 0.08~0.12g / ml (preferably 0.1g / ml), and the concentration of the silk fibroin is 0.04~0.06g / ml (preferably 0.05g / ml) ).

[0042] If the concentration of the polycarbonate polyurethane is too small, the voltage will need to be increased during spinning, which will cause the spinning on the receiving rod to drift non-randomly, resulting in a decrease in the porosity of the inner layer of blood vessels. If the concentration of silk fibroin is too small, the biocompatibility of the inner layer of blood vessels will be poo...

Embodiment 1

[0058] Embodiment 1, double-layer artificial blood vessel and preparation method thereof

[0059] 1. Dissolve 2 g of PCU (polycarbonate polyurethane) and 1 g of SF (silk fibroin) in 20 ml of DMF (HFIP (hexafluoroisopropanol)), and use a magnetic stirrer overnight to fully dissolve in the solvent to obtain polycarbonate polyurethane Mixed solution with silk fibroin; dissolve 0.2 g of Hep (sodium heparin) in formic acid solution to obtain sodium heparin solution, and mix the above two solutions to obtain electrospinning solution A.

[0060] 2. Dissolve 2 g of FPU (fluorinated polyurethane) in 20 ml of HFIP (hexafluoroisopropanol) (DMAc) to obtain a fluorinated polyurethane solution, take 4 g of CS and dissolve it in 20 ml of formic acid to obtain a CS solution, and combine the above two solutions Mix to obtain Electrospinning Solution B.

[0061] After completing the above solution configuration:

[0062] 3. Inhale the electrospinning solution A into a disposable syringe, driv...

Embodiment 2

[0065] Embodiment 2. Double-layer artificial blood vessel and preparation method thereof

[0066] In this example, the concentration of the polycarbonate polyurethane is 0.08 g / ml, and the concentration of the silk fibroin is 0.04 g / ml. The concentration of heparin sodium in the heparin sodium solution is 0.02 g / ml. The concentration of fluorinated polyurethane in the electrospinning solution B was 0.08 g / ml. The concentration of the chitosan solution was 0.02 g / ml. In the obtained blood vessel inner layer, the volume ratio of the mixed solution of the polycarbonate polyurethane and the silk fibroin to the heparin sodium solution is 5:1. The volume ratio of the fluorinated polyurethane solution and the chitosan solution is 5:1.

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Abstract

The method comprises the following steps: dissolving polycarbonate polyurethane and silk fibroin in HFIP (hexafluoroisopropanol) together, dissolving heparin sodium in a formic acid solution to obtain a heparin sodium solution, and mixing the two solutions to obtain an electrostatic spinning solution A; dissolving fluorinated polyurethane in HFIP (hexafluoroisopropanol), dissolving chitosan in formic acid to obtain a chitosan solution, and mixing the two solutions to obtain an electrostatic spinning solution B; the electrostatic spinning solution A is subjected to an electrostatic spinning method to obtain an electrostatic spinning membrane serving as a blood vessel inner layer; the electrostatic spinning solution B is electrospun to the outer surface of the inner layer of the blood vessel, and the double-layer artificial blood vessel is obtained. The inner layer of the blood vessel has good hydrophilicity and biocompatibility to promote formation of the endodermis, the outer layer of the blood vessel has super-strong hydrophobicity to prevent blood permeation, and in-vivo graft monitoring can be directly carried out through an MRI (Magnetic Resonance Imaging) technology.

Description

technical field [0001] The invention relates to the technical field of artificial blood vessels, in particular to a double-layer artificial blood vessel and a preparation method thereof. Background technique [0002] The annual incidence of macrovascular disease is about 5-10 / 100,000 population, and the incidence has increased significantly in recent years. Vascular grafts are the key to treating such diseases. The blood vessels available for clinical application are mainly tissue engineered blood vessels. According to the 2019 China Tissue Engineering Vascular Industry Research Report, the annual demand for tissue engineered blood vessels in China is about 400,000-600,000 pieces. Currently, imported brands such as Terumo, Marque, and GORE-TeX occupy nearly 90% of the domestic market share, and It has been in a state of short supply for a long time. The tissue-engineered blood vessels currently on the market are mainly composed of expanded polytetrafluoroethylene (ePTFE)....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/22A61L27/20A61L27/50D04H1/728
CPCA61L27/18A61L27/227A61L27/20A61L27/507D04H1/728A61L2430/22A61L2300/404A61L2300/42C08L75/04C08L89/00C08L5/10
Inventor 刘金平张力秦进发杨明远刘文豪崔金海代松
Owner ZHONGNAN HOSPITAL OF WUHAN UNIV
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