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Barrier-stem cell homing bionic micro-fluidic chip and application thereof

A microfluidic chip, stem cell technology, applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve the problem of ignoring the relationship between organs/tissue-organ barrier and so on

Pending Publication Date: 2022-08-05
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most organ-on-a-chip research is limited to in vitro modeling of individual organ functions (or functional interfaces), which ignores the interrelationships of organ / tissue-organ barriers, and in fact

Method used

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  • Barrier-stem cell homing bionic micro-fluidic chip and application thereof
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  • Barrier-stem cell homing bionic micro-fluidic chip and application thereof

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Embodiment 1

[0037] The invention provides a barrier-stem cell homing bionic microfluidic chip, comprising a barrier chip and a stem cell homing chip. The barrier chip is cultured in layers from the lower layer to the upper layer according to the cell composition sequence of the barrier structure of the target organ. The barrier chip is used to construct the key functional interface of the target organ and realize the complex organ barrier function between cell-cell / tissue / organ-microenvironment.

[0038] The upper chamber of the stem cell homing chip is used for stem cell culture, the middle chamber is embedded in a 3D "nest" formed by the mammalian acellular matrix corresponding to the target organ, and the lower chamber is a perfusion chamber, which is formed by the upper chamber of the barrier chip. The effluent cell culture medium was perfused. The stem cell homing chip is used to simulate the homing of stem cells. Under the action of various factors, the pluripotent stem cells in the...

Embodiment 2

[0044] like image 3 As shown, the barrier chip is separated from the upper chamber and the lower chamber by a porous membrane 2. In this embodiment, the upper chamber is arranged on the back of the interface layer 1, the interface is arranged on the top surface of the interface layer, and the lower chamber is arranged on the base Bottom layer 4 inside.

[0045] The barrier chip is cultured in layers from the lower layer to the upper layer according to the order of cell composition of the barrier structure of the target organ. For example, a blood-brain barrier chip is prepared, cerebral microvascular endothelial cells and cerebral perivascular cells are cultured in the lower chamber, and astrocytes are cultured in the upper chamber.

[0046] The interface layer is provided with a first inlet and a first outlet, which are communicated with the upper chamber, and a second inlet and a second outlet are arranged on the interface layer of the barrier chip, which are communicated ...

Embodiment 3

[0050] like Figure 4 , Figure 5 As shown, the stem cell homing chip is separated from the lower chamber and the middle chamber by a first porous membrane, and the middle chamber and the upper chamber are separated by a second porous membrane, and a third layer is provided on the interface layer of the stem cell homing chip. The inlet and the third outlet are communicated with the lower chamber, and the lower chamber can be perfused separately, and the perfusion fluid is the cell culture fluid or biochemical reagents flowing out from the upper chamber of the barrier chip. The interface layer of the stem cell homing chip is provided with a fourth inlet and a fourth outlet, which are communicated with the middle-layer chamber, and the middle-layer chamber can be perfused separately, and the perfusion liquid can be culture medium or other liquid biochemical reagents. A fifth inlet and a fifth outlet are provided on the interface layer of the stem cell homing chip, and the conne...

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Abstract

The invention provides a barrier-stem cell homing bionic micro-fluidic chip, which comprises a barrier chip and a stem cell homing chip, the barrier chip performs layered culture from the lower layer to the upper layer according to the cell composition sequence of the barrier structure of a target organ; the upper-layer chamber of the stem cell homing chip is used for culturing stem cells, the middle-layer chamber of the stem cell homing chip is used for embedding an acellular matrix of a mammal corresponding to a target organ to form a 3D'nest ', the lower-layer chamber is a perfusion chamber, and cell culture fluid flowing out of the upper-layer chamber of the barrier chip is used for perfusion. The micro-fluidic bionic chip can simulate a complex and dynamic microenvironment required by an organ, for example, fluid conveying, cell culture and detection units and the like can be designed, a continuous and controllable flowing environment and mutually communicated culture units can be realized, and the micro-fluidic bionic chip is used for highly restoring the physiological characteristics of the target organ. The barrier function of the target organ and the biological process of stem cell homing are simulated.

Description

technical field [0001] The invention belongs to the field of organ chips, and in particular relates to a barrier-stem cell homing bionic microfluidic chip and its application. Background technique [0002] Conventional life science research vehicles are basically based on animals and two-dimensional (2D) cells. Although animal models have achieved human understanding of rich physiology and diseases and developed new drugs, animal models, as a vehicle for human research, have many shortcomings, such as drug candidates may be due to lack of efficacy in animals, or in Discontinued due to the discovery of hazards or toxicity in animals that may not be relevant to humans. Despite significant advances in in vitro biology and toxicology over the past 20 years, more than 80% of investigational drugs currently fail in clinical trials, 60% due to lack of efficacy and another 30% due to toxicity. 2D cell culture has been used in life science research for over a century. However, thi...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/42
CPCC12M21/08C12M23/16C12M23/34C12M25/04C12M35/02
Inventor 邓玉林赵毅蒙吕雪飞李喆高戈陈钰周迪黎朝
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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