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Fermentation medium and fermentation method of Hainanmycin

A fermentation medium and technology of Hainanmycin, which is applied in the field of fermentation medium of Hainanmycin, can solve the problems of low quality of finished products, high cost of raw materials and high power consumption, so as to reduce production cost and power consumption, and reduce cost and power. Consumption, the effect of improving the quality of finished products

Pending Publication Date: 2022-08-05
山东胜利生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This application provides a fermentation medium and a fermentation method of Hainanmycin to solve the problem that the input of basic materials in the fermentation medium in the existing Hainanmycin fermentation process is relatively large, resulting in high raw material costs and low fermentation titer. Low, high power consumption and low quality of finished products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take the Streptomyces orientalis FCD21001-1 glycerol tube and inoculate it into the shake flask medium, the inoculum amount is 0.4%, and the shaking culture is 200 rpm, 5cm amplitude, 44-48h cycle, and the bacterial concentration reaches more than 30% at the end of the culture. The pH reached above 7.50, and the shake flask seed solution was obtained.

[0033] The medium ratios of the first and second seed pots are: corn starch 2.0%, soybean meal powder 2.5%, glucose 2.0%, calcium carbonate 0.8%, yeast powder 2.0%, magnesium sulfate 0.1%, and sodium chloride 0.5% %, amylase is 0.1% of corn starch, calcium carbonate is 0.8%, drinking water is prepared, and pH is 7.0-7.5. The volume of the primary tank is 0.4 tons, and the volume of the secondary tank is 4 tons.

[0034] The inoculation amount of the first-class seed tank is 0.4%. In the first-class seed tank, the first-class seed liquid is obtained by culturing at 28±1℃ and 1:1 gas ratio for 34-40 hours; above 7.50. T...

Embodiment 2

[0039] Take the Streptomyces orientalis FCD21001-1 glycerol tube and inoculate it into the shake flask medium, the inoculum amount is 0.4%, and the shaking culture is 200 rpm, 5cm amplitude, 44-48h cycle, and the bacterial concentration reaches more than 30% at the end of the culture. The pH reached above 7.50, and the shake flask seed solution was obtained.

[0040] The medium ratios of the first and second seed pots are: corn starch 2.0%, soybean meal powder 2.5%, glucose 2.0%, calcium carbonate 0.8%, yeast powder 2.0%, magnesium sulfate 0.1%, and sodium chloride 0.5% %, amylase is 0.1% of corn starch, calcium carbonate is 0.8%, drinking water is prepared, and pH is 7.0-7.5. The volume of the primary tank is 0.4 tons, and the volume of the secondary tank is 4 tons.

[0041] The inoculation amount of the first-class seed tank is 0.4%. In the first-class seed tank, the first-class seed liquid is obtained by culturing at 28±1℃ and 1:1 gas ratio for 34-40 hours; above 7.50. T...

Embodiment 3

[0046] Take the Streptomyces orientalis FCD21001-1 glycerol tube and inoculate it into the shake flask medium, the inoculum amount is 0.4%, and the shaking culture is 200 rpm, 5cm amplitude, 44-48h cycle, and the bacterial concentration reaches more than 30% at the end of the culture. The pH reached above 7.50, and the shake flask seed solution was obtained.

[0047] The medium ratios of the first and second seed pots are: corn starch 2.0%, soybean meal powder 2.5%, glucose 2.0%, calcium carbonate 0.8%, yeast powder 2.0%, magnesium sulfate 0.1%, and sodium chloride 0.5% %, amylase is 0.1% of corn starch, calcium carbonate is 0.8%, drinking water is prepared, and pH is 7.0-7.5. The volume of the primary tank is 0.4 tons, and the volume of the secondary tank is 4 tons.

[0048] The inoculation amount of the first-class seed tank is 0.4%. In the first-class seed tank, the first-class seed liquid is obtained by culturing at 28±1℃ and 1:1 gas ratio for 34-40 hours; above 7.50. T...

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Abstract

Compared with the original fermentation medium, the fermentation medium provided by the invention has the advantages that the use of carbon sources such as starch and glucose is reduced, but different varieties of nitrogen sources, namely high-quality and easy-to-use fish peptone and methyl donor methionine, are added, so that the fermentation efficiency is improved; and the biosynthesis is faster and earlier. Compared with an existing Hainanmycin fermentation process, when the fermentation medium is used for fermenting Hainanmycin, the concentration of thalli is reduced to 42-44% from original 50%, so that the raw material cost and the power consumption are reduced, meanwhile, the average fermentation liquor titer reaches 10413 u / ml or above, and compared with original fermentation liquor titer 9250 u / ml, the fermentation titer is increased by about 12%. Therefore, when the fermentation culture medium is used for fermenting Hainanmycin, less raw material investment is used, higher fermentation titer is obtained, the production cost and power consumption are reduced, and meanwhile, the quality of a finished product is also improved to a certain extent.

Description

technical field [0001] The application relates to the technical field of microbial fermentation, in particular to a fermentation medium and fermentation method of Hainanmycin. Background technique [0002] Hainanmycin sodium is a polyether antibiotic, which is extracted from the culture solution of a Streptomyces orientalis variant isolated from the soil of Hainan Island, my country. It has a strong insecticidal effect on livestock and poultry coccidia, and It has the effect of improving the utilization rate of cattle and sheep feed, and promoting animal growth (weight gain) as a "long meat". [0003] In the existing Hainanmycin fermentation process, the input amount of basic materials in the fermentation medium is relatively large, resulting in high power consumption in the fermentation process. In addition, when the basic material in the fermentation medium is sufficient or abundant, the fermentation titer still does not reach a high level. Therefore, at present, the inpu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12N1/20C12R1/465
CPCC12P17/181C12N1/20Y02P60/87
Inventor 张翠芬梁景乐何鲁波苏薇朱明贺刘正光高鹏
Owner 山东胜利生物工程有限公司
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