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Antisebum and antioxidant composion

A technology of composition and cosmetic composition, applied in the direction of skin care preparations, cosmetics, plant raw materials, etc., can solve the problem of no disclosed anti-oxidant activity, no disclosed guggal anti-sebum activity, no disclosed alcohol fraction or low molecular weight fraction Methods of preparation, uses, and activities to reduce the appearance of wrinkles and aged skin, improve radiance, and improve oil control

Inactive Publication Date: 2004-06-09
UNILEVER NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above documents disclose neither the anti-sebum activity nor the antioxidant activity of guggal or guggal or its fractions
In addition, as far as the fraction of gugulipid is concerned, the above-mentioned documents only disclose the preparation method and use of the fraction rich in gugusterone (ethyl acetate), but do not disclose the preparation method of the alcohol fraction or the low molecular weight fraction , use and activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] This example illustrates the procedure for fractionating gugulipids into fractions of increasing polarity (procedure A) and the procedure for preparing low molecular weight fractions of gugulipids (procedure B).

[0070] Procedure A

[0071] raw material:

[0072] Guguzhi (batch number 42941, Lipo Chemicals Inc. Paterson NJ) medium pressure column (5cm inner diameter×62cm length)

[0073] Silica (gel, Merck), Aldrich, Cat no. 22, 719-6, Grade 9385, 230, 400, mesh 60A.

[0074] Thin layer chromatography plate, LHP-kk20×10cc batch number 004966, Cat#4805-711Whatman.

[0075] Hexane, HPLC grade (Fisher)

[0076] Ethyl alcohol, HPLC grade (Fisher)

[0077] Methanol, HPLC grade (Fisher)

[0078] Trichloromethane, HPLC grade (Fisher)

[0079] Phosphoric Acid (Fisher)

[0080] Copper sulfate CuSO 4 (Fisher)

[0081] Petroleum Ether (Fisher)

[0082] 24 beakers (400ml)

[0083] Scintillation tube

[0084] Capillary (5μl)

[0085] 2 measuring cylinders (2000ml)

[0086] 1 unfolding ...

Embodiment 2

[0154] This example reports an in vitro analysis of the sebum inhibitory effect of gugulipid and its various fractions.

[0155] In vitro test of fat formation by sebocytes

[0156] Human sebaceous glands were isolated from the nose of a male (60 years old) and cultured using immersion tissue culture technique (Bajor et al, J. Invest Dermatol .102: 1994, P.564). These sebocytes accumulate the characteristics of intracellular lipid droplets of mature human sebum.

[0157] The harvested and passaged sebocytes were added to each well of a 48-well tissue culture dish, and heated at 7.5% CO 2 Incubate at 37°C for 10 days in the presence of. On the day of the experiment, the growth medium was removed and the sebocytes were washed three times with phosphate buffered saline (PBS). 0.5 ml of fresh PBS was added to each well at various concentrations shown in Table 1, and 10 μl of the agent to be tested was added. Use the same sample for all three wells. The control sample consis...

Embodiment 4

[0176] This example reports the chemical test and in vitro analysis of the antioxidant activity of gugulipid and its various fractions.

[0177] Chemical test:

[0178] The chemical test measured the antioxidant activity of the various test compounds indicated in Table 3 (except for the low molecular weight fractions tested at 1.67% and 0.17% concentrations, all others were tested at 0.08% concentration). Solubilize 2.2'-azide-bis-(3-ethylbenzothiazoline sulfonate) (6.1μmol / l) and metmyoglobin in phosphate buffered saline (5mmol / l, pH 7.4) ) (610μmol / l). The test substance was then added and the absorbance at 734 nm was measured before and after the addition of the matrix, hydrogen peroxide (250 μmol / l). Subtract the initial absorbance from the absorbance containing the matrix. This prevents the difference in absorbance caused by the test compound itself. The absorbance changes over time, so the absorbance at multiple times was checked. The results are expressed in% of oxidation rel...

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PUM

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Abstract

Gugulipid (a lipophilic ethyl acetate extract from C. mukul or C. wightii) and an alcoholic fraction of gugulipid as antisebum and / or antioxidant active in cosmetic skin care compositions and methods is disclosed. Gugulipid from C. mukul and its alcoholic fraction are capable of delivering dual benefit to the skin: controlling or preventing sebum secretion (oily skin conditions) and protecting the skin from free radical damage.

Description

Invention field [0001] The present invention relates to methods and compositions for controlling or preventing sebum secretion by sebocytes, controlling or preventing oily skin conditions and protecting the skin from free radical activity. Background of the invention [0002] Sebum is skin oil produced by sebocytes (cells of the sebaceous glands in the skin) and then secreted onto the surface of the skin. Excessive sebum on the surface of the skin results in a state known as "oily skin". Oily skin has shiny, undesirable appearance and unpleasant touch. There are many existing methods and compositions that have tried to control excessive sebum secretion, but none of them can completely provide satisfactory results. [0003] The formation of free radicals in the skin does not seem to be related to sebum secretion. Low levels of free radicals are formed in the skin as part of the natural metabolic pathway. The increase in the level of free radicals is affected by ultraviolet radiati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/00A61K8/92A61K8/96A61K8/97A61K36/328A61Q5/00A61Q19/00
CPCA61K2800/522A61Q19/00A61K8/922A61Q19/008
Inventor J·P·麦库克J·M·科雷伊P·L·多罗吉J·S·巴约尔H·E·克纳格斯B·A·朗格E·夏佩M·塔尔曼
Owner UNILEVER NV
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