The invention provides a display substrate, a preparation method thereof and a
display device. The display substrate comprises a first
electrode layer located on one side of a driving backboard, and the first
electrode layer comprising a plurality of first electrodes distributed in an array mode; an electric leakage
cut-off layer, located on the sides, deviating from the driving backboard, of thefirst
electrode layers, the electric leakage
cut-off layer being located between every two adjacent first electrodes, and
cut-off grooves and cut-off protrusions located on the two sides of the cut-off grooves being formed in the surfaces of the sides, deviating from the driving backboard, of the electric leakage cut-off
layers; a light-emitting function layer, located on the side, deviating fromthe driving backboard, of the electric leakage cut-off layer and the first electrode layer; and a second electrode layer positioned on one side, deviating from the driving back plate, of the light-emitting function layer. According to the display substrate, when one pixel emits light, current cannot be transmitted to other adjacent pixels through the
hole injection layer, the phenomena of transverse electric leakage and
crosstalk are avoided, and the quality and the
color purity of a display are improved.