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Magnetic compound microsphere of blot gel for biological macromolecular template and its reverse-phase suspension polymerization process

A technology of magnetic composite microspheres and template imprinting, which is applied in the fields of material science and engineering and biological separation engineering, can solve the problems of limited swelling of template imprinted gel composite microspheres, and achieves the effects of easy coating, rapid separation and convenient preparation.

Inactive Publication Date: 2004-11-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention needs to solve the limited swelling problem of the template imprinted gel composite microspheres to ensure that the shape and size of the imprinted holes are relatively stable during the imprinting and recognition process of biomacromolecules

Method used

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  • Magnetic compound microsphere of blot gel for biological macromolecular template and its reverse-phase suspension polymerization process
  • Magnetic compound microsphere of blot gel for biological macromolecular template and its reverse-phase suspension polymerization process
  • Magnetic compound microsphere of blot gel for biological macromolecular template and its reverse-phase suspension polymerization process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Preparation of bovine serum albumin template imprinted gel composite microspheres

[0041] Add 100ml of toluene to a 250ml three-neck flask equipped with a stirrer and vent tube, add 0.2g of ethyl cellulose, and stir to dissolve it. Add 9gAM, 1g Bis and 0.0330g bovine serum albumin (BSA) into 20ml water, dissolve and add 0.2g ground magnetic Fe 3 o 4 Powder, stir and disperse evenly, add to the three-necked flask, continue to stir; dissolve 0.1g of potassium persulfate and 0.05g of sodium bisulfite in 10ml of water, and drop into the three-necked flask. Nitrogen was passed through to remove oxygen, and the reaction was carried out at room temperature for 2 hours. After the reaction, the organic solvent part was filtered out with a 180-mesh screen to obtain BSA template imprinted gel composite microspheres.

[0042] 2. Elution of bovine serum albumin

[0043] After the prepared template-imprinted gel composite microspheres were washed with acetone, they were washe...

Embodiment 2

[0046] 1. Preparation of lysozyme template imprinted gel composite microspheres

[0047] Add 100ml of toluene to a 250ml three-neck flask equipped with a stirrer and vent tube, add 0.2g of ethyl cellulose, and stir to dissolve it. Add 9gAM, 1g Bis and 0.0144g lysozyme to 20ml water, dissolve and add 0.2g ground magnetic Fe 3 o 4 Powder, stir and disperse evenly, add to the three-necked flask, continue to stir; dissolve 0.1g of potassium persulfate and 0.05g of sodium bisulfite in 10ml of water, and drop into the three-necked flask. Nitrogen was passed through to remove oxygen, and the reaction was carried out at room temperature for 2 hours. After the reaction, the organic solvent part was filtered out with a 180-mesh sieve to obtain the lysozyme template-imprinted gel composite microspheres.

[0048] 2. Elution of lysozyme

[0049] After the prepared gel composite microspheres were washed with acetone, they were washed repeatedly with distilled water. Then soak it in an ...

Embodiment 3

[0052] 1. Preparation of Ovalbumin Template Imprinted Gel Composite Microspheres

[0053] Add 100ml of benzene into a 250ml three-neck flask equipped with a stirrer and vent tube, add 0.2g of methylcellulose, and stir to dissolve it. Add 9gAM, 1g Bis and 0.0215g ovalbumin to 20ml water, dissolve and add 0.2g ground magnetic Fe 2 o 3 Powder, stir and disperse evenly, add to the three-necked flask, continue to stir; dissolve 0.1g of potassium persulfate and 0.05g of sodium bisulfite in 10ml of water, and drop into the three-necked flask. Nitrogen was passed through to remove oxygen, and the reaction was carried out at room temperature for 2 hours. After the reaction, the organic solvent part was filtered out with a 180-mesh sieve to obtain the ovalbumin template-imprinted gel composite microspheres.

[0054] 2. Elution of ovalbumin

[0055] After the prepared gel composite microspheres were washed with acetone, they were washed repeatedly with distilled water. Then soak it ...

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Abstract

A composite magnetic gel microsphere for biological macromolecualr template blot, based on the template blot (molecular blot) technique, is prepared from different biological macromolaculate as template molecule and different metals (or their oxides) as magentic component through reversed-phase suspension polymerization. It can be used for separating biological macromoleculae with high precision and simple process.

Description

technical field [0001] The invention belongs to the fields of material science and engineering and biological separation engineering, and more precisely relates to a biomacromolecule template imprinted gel magnetic composite microsphere and a preparation method thereof by reversed-phase suspension polymerization. Background technique [0002] Molecular recognition and recognition specificity are ubiquitous and unique characteristics of the whole biology, and play a special and important role in biological evolution. Template imprinting is a technology based on biological bionics, using artificial methods to prepare polymers with recognition specificity for specific molecules (template molecules or imprinted molecules). Recently, molecularly imprinted polymers (MIPs) prepared by this technology have shown wide application prospects in separation and purification, immunoassay, simulated enzymes, and biosensors, and have become one of the most promisin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D71/00C08F2/12C08F2/44C08K3/22
Inventor 成国祥陆书来庞兴收张立广
Owner TIANJIN UNIV
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