Method for preventing plant indefinite bud and cultivating seedling in glass container

Abstract

Through improving traditional MS culture medium, adding single or mixed RE element compound, such as nitrate, chloride or oxide of La, Pr, Nd and Sm, and / or kelp sulfate polysaccharide; and reducing or elimianting NAA and 6-BA hormone, the plant organ is cultured in the said culture medium. The method of the present invention can reduce plant indefinite bud and germchit vitrification.

Description

field of invention [0001] The invention belongs to a method for plant cultivation in biochemical engineering technology, in particular to a method for preventing adventitious buds of plants [0002] And the method for seedling culture vitrification. Background technique [0003] In plant tissue culture, for example, vitrification has been found in dozens of plant cultures such as carnation, cauliflower and petunia. The characteristics of vitrification are: the culture is short and swollen, translucent glass-like; the leaves are light green or yellow-green, the leaves are thick and narrow, sometimes the base is wide, fragile and brittle, and the leaf surface lacks cuticle and waxy or waxy Incomplete development; the stem is short, thick and flat, and the internodes are very short; through dissection, it is further found that the vitrified culture has no functional stomata in its leaves, which leads to the dysfunction of the guard cells and cannot close the stomata. At the sa...

AI Technical Summary

Problems solved by technology

The study on the relationship between vitrified tissue and its culture environment showed that low temperature treatment and Cl - , NO 3 - , Ca 2+ Plasma has no effect on the vitrification of artichokes, and the formation of vitrification is related to the water, nutritional conditions, and hormone levels in the growth environment of plant tissues; once vitrification is formed, sometimes improving a certain condition may alleviate the degree of vitrification, but it is difficult to solve it completely

[0009] At present, methods such as reducing the hormone level of the medium to prevent abnormal dedifferentiation, reducing the water content in the medium, avoiding high temperature and purifying the culture are generally used to prevent vitrification, but these methods are often used in adventitious bud culture and seedling propagation. It is not suitable for reducing and preventing the occurrence of vitrification. For example, in the liquid suspension culture of A. annua clustered buds, the occurrence of culture vitrification is a common problem. The suitable temperature for the growth of A. annua buds is 25-28°C. The optimum temperature for the synthesis of artemisinin is around 30°C, and the hormones NAA and 6-BA are required for differentiation. Traditional methods such as lowering the culture temperature, canceling or reducing the amount of hormones cannot be used to reduce or prevent the occurrence of vitrification. Therefore, it is necessary to Explore a new method to prevent vitrification of plant adventitious buds and seedling culture

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