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Lactobacillus fixed cell in-situ separating-fermenting lactic-acid production process

A technology of immobilized cells and in-situ separation, applied in the field of lactic acid preparation, can solve the problems that fermentation broth cannot pass continuously and evenly, block the entrance of resin column, and reduce active cells, and achieve long service life, low consumption and fast proliferation. Effect

Inactive Publication Date: 2005-11-02
TIANJIN GUARD T & D
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods still have the following disadvantages: during the fermentation process, the active cells in the fermentation broth are reduced, the conversion rate is low, the loss of lactic acid is too large, the energy consumption is large, and the cost is high.
The disadvantage of the former method is that the anion exchange resin absorbs lactic acid from the fermentation broth flowing through, and at the same time, bacteria and nutrients also flow into the resin column and are absorbed or retained. It will block the inlet of the resin column, so that the fermentation broth cannot pass through continuously and evenly.
The disadvantage of the latter method is that using methanol as the eluent for lactic acid elution is neither economical nor environmentally friendly.

Method used

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  • Lactobacillus fixed cell in-situ separating-fermenting lactic-acid production process
  • Lactobacillus fixed cell in-situ separating-fermenting lactic-acid production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A. Cell immobilization

[0026] (a) Preparation of bacterial suspension: Cultivate lactic acid bacteria to the end of exponential growth, centrifuge for 10 minutes with a centrifugal force of 9000g, discard the supernatant, and disperse the bacteria suspension with an equal weight of sterilized normal saline. Take 22~38.7 grams of the bacterial suspension;

[0027] (b) Preparation of a mixture of embedding agent and bacterial suspension: Dissolve 8-12 grams of polyvinyl alcohol and 2-4 grams of sodium alginate in 100 milliliters of distilled water, heat in a water bath to dissolve them, and sterilize after mixing. After cooling to 40°C, add 22~38.7 grams of sterilized 2% carrageenan distilled water solution to the bacterial suspension of (a). The weight ratio is bacterial suspension: 2% carrageenan solution: 8-12 % Polyvinyl alcohol and 2~4% sodium alginate mixture = 1:1:3~5 mix well;

[0028] (c) Preparation of cross-linking agent: Weigh 2-8 grams of calcium chloride and 1...

Embodiment 2

[0039] Choose polyvinyl alcohol, sodium alginate, and carrageenan to formulate four embedding agents in different proportions according to the percentage concentration in Table 1, which are used to make bead-type immobilized cell granule beads. Use the following method to test the embedded bacteria Bead strength: Place embedded particles of equal size between two glass slides, put weights on the glass slides until the particles are crushed, and use the weight of the weights to characterize the strength of the particles and measure its various indicators. The results are shown in Table 1.

[0040] Preface

[0041] Experiments have proved that using a single calcium alginate or alginic acid as an embedding agent can hardly form beads. When polyvinyl alcohol or sodium alginate is used as an embedding agent to prepare beads, the embedding agent tends to stick together when cross-linked to form a large aggregate. When carrageenan and sodium alginate are used as embedding agen...

Embodiment 3

[0043] Lactic acid was produced according to the process of Example 1, and the immobilized lactic acid bacteria beads of the present invention were repeatedly used three times. Under exactly the same conditions, unimmobilized lactic acid bacteria beads are used for production. The product concentration results are shown in Table 2.

[0044] reaction system

[0045] It can be seen from the test results that, although the product concentration of the immobilized lactic acid bacteria beads of the present invention is slightly lower than that of the unimmobilized bacteria liquid when used for the first time, it can be reused and can maintain reactivity.

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Abstract

The invention refers to a process for using yeasting method to produce lactic acid. Takes out the mixed liquid of embedding agent and fungus suspending liquid to solidify the cells, the embedding agent is made up of fungus suspending liquid: 2% kala glue liquid: 8-12% polyvinyl alcohol and 2-4 sodium alginate with proportion 1:1:3-5, combines with using pH controller to control the acid rate pH value = 5.0-6.2 automatically, when the pH gauge displays the pH value is 5.0 in the yeasting liquid, the pH control system switches on the power automatically, the circular pump begin to work, when the pH value reaches 6.5, the relay cuts off the power, the circular pump stops, the yeasting is going on, it realizes in situ separation yeast lactic acid generating.

Description

Technical field [0001] The technical solution claimed by the present invention relates to a preparation method of lactic acid, specifically a process for producing lactic acid by a fermentation method. Background technique [0002] Many of the biological fermentation and biocatalysis are product inhibitory processes. If the products are not neutralized or separated in time, the fermentation reaction process will not continue. Lactic acid fermentation is a typical product inhibitory fermentation process. The suitable acidity for lactic acid fermentation is pH=5.0-6.2. When the pH value is less than 5, fermentation is inhibited and the lactic acid yield decreases. In the process of lactic acid fermentation, due to the accumulation of lactic acid, the pH value of the fermentation broth gradually decreases, which severely inhibits the growth of lactic acid bacteria and the synthesis of lactic acid. When traditional fermentation method is used to produce lactic acid, in order to elimi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/02C12P7/56
Inventor 马建芳李剑徐子钧王仁静孙雪莲李明智刘如林
Owner TIANJIN GUARD T & D
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