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Methyl eicosapentaenoic acid preparing and purifying method from pavlova viridis

A technology of methyl eicosapentaenoate and Pavlova green algae, applied in chemical instruments and methods, preparation of carboxylate, preparation of organic compounds, etc., can solve the problems of low EPA yield and purity, and high cost, Achieve the effect of low cost, low consumption and high purity

Inactive Publication Date: 2005-12-28
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield and purity of these methods or EPA are low, or the cost is too high

Method used

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  • Methyl eicosapentaenoic acid preparing and purifying method from pavlova viridis
  • Methyl eicosapentaenoic acid preparing and purifying method from pavlova viridis
  • Methyl eicosapentaenoic acid preparing and purifying method from pavlova viridis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Algae Cultivation and Harvesting

[0059] The unicellular marine microalga Pavlova viridis was purchased from the Institute of Oceanology, Chinese Academy of Sciences, Qingdao, rejuvenated and cultured in our laboratory, and stored at 21-27°C. The culture conditions are as follows: 6000-9000lux, 12:12L / D, aeration culture. Harvest at the end of exponential growth, centrifuge at 4000-8000g for 8-15 minutes to collect algae, wash with distilled water to remove salt and other impurities, freeze-dry, and store in low temperature freezer.

Embodiment 2

[0060] Embodiment 2. Preparation of silver-coated silica gel column:

[0061] Add a certain amount of silica gel for chromatography to twice the volume of absolute ethanol, stir and mix to form a suspension. Dissolve silver nitrate in an appropriate amount of 70% ethanol, add the above chromatographic silica gel and mix well. The weight ratio of silver nitrate to silica gel is 1:10. Then the ethanol was evaporated, and the obtained silver-coated silica gel was placed in an oven, activated at a high temperature of 120°C (more than 12 hours), cooled, and stored in a dark place.

Embodiment 3

[0062] Example 3. Preparation and purification of eicosapentaenoic acid methyl ester from Pavlova viridis

[0063] Preparation and purification of eicosapentaenoic acid methyl ester from Pavlova viridis, it comprises the following steps:

[0064] Step 1. Put 1.0 g of green Pavlova freeze-dried algae powder into a screw-top test tube, add 20 ml of 5% chloroacetyl-methanol (ie 1:20 v / v), fill it with nitrogen and seal it, and store it at 80°C Reacted for 2 hours, cooled to room temperature,

[0065] Step 2. Add equal volumes of double-distilled water and n-hexane to the reaction mixture obtained in step 1, shake and extract, absorb the upper liquid, then add an equal amount of n-hexane to repeat the extraction until the supernatant is no longer yellow,

[0066] Step 3. After combining the extracts, dry with a desiccant, filter the desiccant, and evaporate n-hexane under the protection of nitrogen to obtain 0.046 grams of fatty acid methyl esters,

[0067] Step 4. the fatty aci...

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Abstract

A method for preparing and purifying eicosapentaenoic acid methyl ester from Pavlova viridis, which is to add 5% chloroacetyl-methanol to the freeze-dried algae powder of Pavlova viridis, airtightly fill it with nitrogen, and store it at 80 React at -100°C for 0.5 to 2 hours, cool to room temperature, add the same volume of double distilled water and n-hexane respectively, shake and extract, extract several times, combine the extracts, evaporate n-hexane under nitrogen protection, and obtain fatty acid methyl ester , and then carry out chromatography with a silver-coated silica gel column, use acetone-n-hexane solution gradient elution, combine the eluent containing methyl eicosapentaenoic acid with a purity higher than 95%, and evaporate acetone and n-Hexane, that is, methyl eicosapentaenoate, the purity is higher than 95%. The method of the invention is simple, easy to implement, low in cost and high in purity of the eicosapentaenoic acid methyl ester.

Description

technical field [0001] The present invention relates to Pavlova and eicosapentaenoic acid, in particular to a method for preparing eicosapentaenoic acid methyl ester from Pavlova. Background technique [0002] Pavlova viridis is a yellow-green microscopic gold algae. The algae are oval or round, without cell walls. The cell diameter of the algae is about 4-6 μm, and it can move. It is a single-celled marine microalgae with a wide temperature range and low light requirements. It is often used as a good bait for shrimp larvae and economic shellfish. Studies in recent years have shown that Pavlova viridis is rich in polyunsaturated fatty acids such as Eicosapentaenoic acid (hereinafter referred to as EPA), and its EPA content accounts for 12-15% of the total lipid (varies according to different culture conditions) . [0003] Polyunsaturated fatty acids such as EPA exist in the glycolipids of algae cells. To obtain methyl eicosapentaenoate, it is generally necessary to extra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C67/08C07C67/48C07C67/58C07C69/587
Inventor 刘志礼李梅曹德华徐瑾薛仁皓
Owner NANJING UNIV