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Application of Actinomucor elegans in use for preparing ceramide

A technology of Yazhi mucormyces radiata and ceramide, applied in the biological field, can solve problems such as threats to the health and life safety of the people, impacts on feed and breeding products export earnings, and excessive residues of prohibited drugs, etc., to reduce feeding costs, low cost, The effect of improving the absorption and utilization rate

Inactive Publication Date: 2005-12-28
SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the illegal use of prohibited drugs has seriously exceeded the standard of prohibited drug residues in aquaculture products. For example, the European Union, Eastern Europe, Japan, Hong Kong and other countries and regions have repeatedly returned the livestock products exported by my country. The European Union also stopped importing Chinese feed in August 1996 due to the excessive use of drugs in Chinese feed and excessive residues. Poultry meat and its related products have caused serious economic losses to the country and also damaged the country's image

Method used

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  • Application of Actinomucor elegans in use for preparing ceramide
  • Application of Actinomucor elegans in use for preparing ceramide
  • Application of Actinomucor elegans in use for preparing ceramide

Examples

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Effect test

Embodiment 1

[0041] The preparation of embodiment 1 ceramide

[0042] The invention provides a method for preparing ceramide, which is prepared by extracting rice bran as a raw material through bio-fermentation technology, and includes the following steps:

[0043] a. Select strains: select Actinomucor elegans from the General Microorganism Center of China Committee for Microbial Culture Collection: AS3.2778; the amount of strains is 4-12g;

[0044] b. Strain fermentation: carry out solid fermentation of selected strains, and the solid fermentation conditions are:

[0045] Solid medium: rice bran 98.43%, yeast extract 0.7%, KH 2 PO 4 0.1%, K 2 HPO 4 0.1%, MgSO 4 0.05%, CaCl 2 0.05%, ZnSO 4 0.02%, rapeseed oil 0.55%;

[0046] Cultivation temperature: 15℃~35℃;

[0047] Culture time: 24~84h;

[0048] Medium pH value: 7.0~8.0;

[0049] Medium water content: 40% to 70%;

[0050] Medium inoculum volume: 4% to 12%.

[0051] The culture medium adopts variable temperature culture,...

Embodiment 2

[0062] Qualitative and quantitative analysis of embodiment 2 product ceramide

[0063] 1. The ceramide prepared by the method of the present invention is detected by the Sichuan Provincial Center for Disease Control and Prevention, and the inspection basis is: GB / T5009.11-1996 and GB4789-94. The hygienic indicators are as follows:

[0064] Product heavy metal residue: see Table 1

[0065] Item Unit Measurement result

[0066] Product microbial indicators: see Table 2

[0067] Item Unit Measurement result

[0068] 2. The ceramide (ceramide) prepared by the method of the present invention is detected by the Waters liquid chromatograph at the Chengdu Branch Analysis and Testing Center of the Chinese Academy of Sciences, wherein the ceramide reference substance is a product of Sigma Company, and by comparing with the reference substance, the ceramide content is 101.8 %, purity and HPLC spectrum are basically consistent with the standard product of Sigma Compan...

Embodiment 3

[0078] Embodiment 3 Preparation of by-product enzyme-type feed additive of the method of the present invention

[0079] The remaining product 98.2~99.6% that embodiment 1 separates gained is added following stabilizer: calcium lactate, sorbitol make enzyme type feed additive stabilizer, the consumption scope of calcium lactate 0.2~0.8%, optimum consumption 0.5%; The dosage range is 0.2-1.0%, and the optimal dosage is 0.6%.

[0080] Preparation method: take 98.9 kg of solid fermentation raw materials before fermentation, calculate the respective addition amounts of calcium lactate and sorbitol, and add 10 times the amount of boiling water to dissolve it based on the total amount of 0.5 kg of calcium lactate and 0.6 kg of sorbitol Finally, it is sprayed on the residue after extracting ceramide, stirred evenly, air-dried at 30°C to 50°C, pulverized, packaged, and tested to become the finished product.

[0081] The beneficial effect of the by-product enzyme-type feed additive pre...

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Abstract

The invention provides a new application of Actinomucor elegans, in particular the application of Actinomucor elegans to produce ceramide. The invention also provides a preparation method of ceramide, which uses rice bran as raw material through fermentation , extracted, separated and purified. Another technical solution of the present invention is to provide a by-product of the ceramide preparation method—an enzyme-type feed additive. The raw material rice bran of the preparation method of the ceramide of the present invention is rich in sources, the ceramide is produced through biotechnology, the cost is low, the safety is good, the ceramide is high in purity, the pollution is small, and the waste residue is fully utilized. The enzyme-type feed prepared by the present invention The additive is used in a small amount, which can significantly improve the absorption and utilization rate of animal feed, reduce the feed-to-meat ratio, reduce feeding costs, and improve production efficiency.

Description

technical field [0001] The invention provides a new application of radiomucor elegans, in particular related to the application of producing ceramide. The invention also provides a preparation method of ceramide, which belongs to the field of biotechnology. Background technique [0002] The strain with the bacterial number AS3.2778 is a typical strain of Actinomucor elegans, which is preserved by the General Microorganism Center of China Committee for the Collection of Microbial Cultures. It is isolated from fermented fermented bean curd and is mainly used in the manufacture of fermented food, especially fermented bean curd. This strain is an excellent strain for brewing fermented bean curd, but other uses of this strain have not been reported yet. [0003] Ceramide can transmit nerve instructions, promote cell proliferation and differentiation, protect the body from external physical (such as ultraviolet rays), chemical (toxic gas), biological and other factors, prevent th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/02
Inventor 刘克武李佳刘鑫
Owner SICHUAN UNIV
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